Structure of Product Description: Column Name Particle diameter(um) Functional Group Column length(mm)x ID(mm)/Art No.
Vydac TP phase specifications
Phase: 218 TP Vydac TP Phase
Phase description :Vydac 218 TO (C18)
Particle Size (um): 5,
Properties: First generation polymeric C18 phase with unique selectivity
Application Benefits: Small polypeptides 4-5K MW, enzymatic digest fragments, natural and synthetic peptides, multi-ring compounds
USP Code: L1
Vydac 218TP and 238TP are used for small polypeptides of 4-5kDa, enzymatic digest fragments, natural and synthetic peptides and multi-ring compounds
Vydac MS phase specifications
Phase: Vydac 218 MS C18
Particle Size (um): 5,10
Properties: Monomeric bonding offers increased peptide interaction and generally yields higher peak counts
Application Benefits: Use for simple enzymatic digests (<12 proteins) or biomolecules 0 – 5k MW
USP Code: L1
Vydac 218MS and 238MS are used for simple enzymatic digests (< 12 proteins) or biomolecules (0-5kDa).
Difference between TP and MS
Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations. The large pores of the 300A TP silica give polypeptide molecules complete access to the interior of the silica pores.
The general features of Vydac TP include:
Long column lifetime and negligible phase leaching
Reliable protein purifications, scalable from analytical to preparative scale
Referenced in a large number of patents and publications
Vydac MS columns are a later development from Grace for the reversed-phase HPLC separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins.
The general features of Vydac MS include:
300A pore size spherical silica
Five reversed-phase chemistries
Excellent peak shape with little or no TFA
High protein recoveries make scale-up easy
The improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics.