Guidelines for PolyCAT A Bulkmaterials
Structure of Product Description:
Bulkmaterial Name – Particle diameter(um)- Pore Size( A)/Article No.
PolyCAT A Bulkmaterials for Cation-Exchange
Uniqueness of PolyCAT A
PolyCAT A™ is made by attaching covalently poly(aspartic acid) to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Moreover, recovery and binding capacity is high.
Overview of Applications of PolyCAT A column for Cation-Exchange
1) Protein variants involving deamidation, PEGylation, determining position of attachment, desialylation and many more. It is widely used by the biotechnology industry.
2) Hemoglobin variant analysis by clinical chemistry labs.
3) Specifically for Proteins with pI above 6.0 (5.0 in special cases).
4) Monoclonal antibodies.
Properties of PolyCAT A column for Cation-Exchange
PolyCAT A™ is a weak cation-exchange (WCX) material. It is used at pH values above 4. A gradient to unbuffered acetic acid will uncharged PolyCAT A™, permitting the elution of proteins in a volatile solvent. See our poster on this subject.
Peptides can be run on PolyCAT A™ if they contain at least two excess positive charges above pH 4. More weakly basic peptides, such as tryptic fragments, are not reliably retained. Instead use PolySULFOETHYL A™ at pH = 2.7 – 3.0
For proteins larger than 20 KDa, we recommend the use of pore diameters particles. Use at least 1000 Å Porediameter for optimal selectivity and efficiency. Our 3-µm material with 1000- or 1500-Å pores is the finest cation-exchanger available for protein separations.