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Newcrom BH HPLC Column

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Description

Newcrom BH HPLC Column is a novel multimode Chromatographgy concept with many benefits.

Structure of Product Description

Column Description-Column ID x Column lengt(mm)-Particle Diameter um – Pore Diameter (A)/Article No.

Newcrom BH  HPLC Column are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm
Column Lengths: 250, 150, 100, 50, 25, 10 mm
Particles: 5 µm and 3 µm
Pores: 100 A

Newcrom BH Application Examples

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Product Design Philosophy

1. Surface Chemistry

Newchrom BH HPLC Column with multifunctional 25A Ligand and terminal functional group

The new surface chemistry is based on the idea of moving the charged embedded functional groups as far from silica gel surface as possible.

This was achieved by attaching a long alkyl chain to the silica gel surface and placing an ion-exchange group at the terminal end of this chain. As a result, the ion-exchange functional groups have high mobility inside the pores of the solid particles. This eliminates the chelating (trapping) effect observed with multi-charged analytes on a surface of traditional ion-exchangers. The embedded ion-exchange groups in Newcrom behave as ions in a solution not as ions on a surface. While highly mobile, they can not go beyond the length of the ligand chain.

Chromatography related to ion-exchange process thus still takes place. Ions of opposite charge can be retained and separated based on their charge, ion size, and polarization ability. Also, reversed-phase nature of the stationary phase allows ion separation based on their hydrophobic properties, charge position in the molecular skeleton and isomeric structure of none-charged part of the molecule.

Additional benefit of these stationary phases is low concentration of buffer in the mobile phase needed to generate efficient ion-exchange. Some amount of formic acid (HCOOH) in the mobile phase is all that’s required to generate efficient ion-exchange process. For low UV applications, small amount of phosphoric acid (H3PO4) works as an efficient mobile phase ionic modifier. Yet another benefit is very short equilibration time for switching from one buffer to another, while on older ion-exchange columns the equilibration usually takes many column volumes to get stable retention.

Methanol and acetonitrile as well as any of their combination can be used to change selectivity of the separation.

2. Column Design

Newchrom material is available in traditional HPLC column as well as in a new column with a new outer design.This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

A column with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

 

Additional information

Weight 500 g
Dimensions 35 × 7 × 5 cm
Product Description

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