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Xtimate Hybride HPLC & UHPLC

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Frequently Asked Questions about Company for Field/Application

            MSD for Silicat

Frequently Asked Questions about Column/Material

  1. g Why can’t I use reversed-phase HPLC for proteins?

2) Do I need to degas mobile phases?

3) How should I store columns?

4) What’s the correct way to prepare a mostly organic mobile phase?

5) Why aren’t my peptides retained on the PolySULFOETHYL A™ column?

6) Can I use TFA or HFBA in the mobile phase using PolyLC for Bio HPLC?

7) My peaks are badly skewed or split in HILIC. Why?

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         Instructions for: Capillary and Microbore Columns

Using & Handling – Brand  

            Initial Use:

Routine Use:

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Column maintenance:

Minimize Iron in the System:

Cation-Exchange of Peptides:

Specific Isolation of Crosslinked Peptides:

Tryptic digests:

Isolation of  from Tryptic Digest:.

Cation-Exchange of Proteins:

Column Leaching:

Literature Links to Knowledge

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