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FlipLC removes impurities

FlipLC removes impurities from natural sources

Samples from natural sources, such as fruits, vegetables, culinary products, water, soil, and bodily fluids, are complex mixtures consisting of multiple compounds. FlipLC method removes impurities from the complex samples, shortens chromatography run time, and increases the lifetime of the columns.

It requires minimum sample preparation. The isolation column flips direction and position in the flow path during this process, that is why we call it FlipLC.

FlipLC empowers measuring charged analytes in complex mixtures

Measuring components in complex samples by HPLC is complicated. Complex samples we find in natural products, body fluids and environmental probes. In such heterogeneous mixtures many components interfere with each other’s. Hence, different sample matrices require different sample cleaning procedures prior to separation. As a result, only one procedure is insufficient across a variety of products. The column’s operating life is usually short, even with sample cleaning. Irreversibly kept contaminants are the reason for that. Thus, long cleaning procedures and guard columns must be employed.  Furthermore, one has to us expensive, and complex compound specific detection techniques such as MS. One example of such problems is measuring of nitrates. All the existing methods are complicated and take considerable time to execute. Usually, the methods are specific only for the particular sample types.

FlipLC™ is an alternative method to avoid the interference of most contaminants.  Before the separation column an isolation column and a high-pressure switching valve must be installed. It is a method that allows sample cleaning and analyte separation in one automated process.  If you are interested in this method, please click here

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