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SieLC Mixed Mode Products

SIELC mixed mode specialist


SIELC Technologies is a mixed mode specialist and pioneer in creating highly innovative HPLC materials and columns. Two highly creative chemists formed the company Vlad Orlowski and Uri Seletionec in Prospect Hights, Il, north of Chicago. In the meantime, Vlad moved on to form his own company Horizon

Nevertheless, SieLC grew further as highly innovative specialist. They expanded by addressing difficult separations with novel technologies and methods. 

We know them since they started.

Product Range

Click buttons below  to go to  individual products:

            Novel Hardwaew and Devices      

                FliPLC to Remove Impurities

  NewChrom novel Ion Exchange Column  

   Legacy Columns to Match USP Methods  

   Obelisc to improve Productivity in HPLC  

        Primesep Multi Mode HPLC Column      

       Promix Biomolecule HPLC Columns        

Shark Column Utilizes Hydrogen Bonding    



Why should you buy from SieLC.

In a world of progreess ther is competition. To escape competition  specialist develop permanently problem solutions. Furthermore, the problems and thus the solution become increasingly complex. Modern  Pharmaceutical molecules  cary in the same construct hydrophibic, hydrophilic, ionic and chiral domains, Similar with phytochemicals. Professional chromatographers avoid comommodity problem solution.  With better column section and multifunctional columns they make th company and their products more unique while at the same time they increase their job security.  Large column companies have changed their focus from novel product development to releasing the specialist, to moving towards using and procuring commodities and to grow by acquiring companies. 

Smaller life science companies grow by creating highly differentiated specialties using more complex production tools. SieLC is permanently pioneering new applications and thus helping professional chromatographers to improvr their job security.

Polyethyleneimine (PEI) by mixed mode HPLC analysis

polyethyleneimine (PEI) has multiple industrial, medical, biological and research applications. It is a difficult compound to analyse by HPLC. The problem has many degrees of difficulty.
PEI is not a monodisperse compound. It is a mixture of different molecular lengths and branching structures.
It has multiple charges in acidic and neutral pH, which is most common in HPLC
PEI molecules have no UV chromophores. In other words, UV-Vis detector cannot measure them. Incorporating a Cu* complex empowers you to use a UV-Vis Detector. For more about this method click here

NewChrom is a new type of mixed-mode HPLC columns

SieLC attached long alkyl chain to the silica gel surface.  The ion-exchange group is at the terminal end of this chain. Consequently, the ion-exchange functional groups have high mobility inside the pores of the solid particles.  This ends the chelating (trapping) of multi-charged analytes on a surface of traditional ion-exchangers. The embedded ion-exchange groups in Newcrom behave as ions in a solution. However, they are mobile, but they cannot go beyond the length of the ligand chain. Read more.

Frequently Asked Questions

What are Mixed or Multi mode materials?    

You find more theoretical and practical knowledge in here  

   MSD for Silicat

Frequently Asked Questions about Column/Material

  1. g Why can’t I use reversed-phase HPLC for proteins?

2) Do I need to degas mobile phases?

3) How should I store columns?

4) What’s the correct way to prepare a mostly organic mobile phase?

5) Why aren’t my peptides retained on the PolySULFOETHYL A™ column?

6) Can I use TFA or HFBA in the mobile phase using PolyLC for Bio HPLC?

7) My peaks are badly skewed or split in HILIC. Why?

Frequently asked Company Admin Questions


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