Vydac 300 Å columns are a commonly employed range in bioseparations. Following the acquisition of Grace HPLC ranges by Hichrom, Vydac HPLC  columns continue to be available exclusively from us in capillary to microbore and analytical to preparative dimensions.

Separate biomolecules from small peptides to large intact proteins with the Vydac family of reversed-phase columns (including Vydac TP, MS, Denali, and Everest) and ion exchange (Vydac 302IC) columns. Reversed-phase columns for a polypeptide separation should be considered on the basis of the polypeptide’s hydrophobicity, with molecular weight as a secondary consideration.

Vydac TP

Vydac TP reversed-phase material consists of aliphatic groups bonded to the surface of 300 Å pore diameter silica. The large pores of the TP silica give polypeptide molecules complete access to the interior of the
silica pores. Vydac TP silica is the standard that has defined large pore HPLC for polypeptide separations for nearly two decades.

Key Features

Long column lifetime and negligible phase leaching
Reliable protein purifications, scalable from analytical to preparative scale
Referenced in a large number of patents and publications

Vydac TP Phase Specification

Product group	Chemistry	Particle Size um	Properties	Applicatons	USP Code
218TP	C18	3, 5, 10	First generation polymeric C18 phase with unique selectivit	Small polypeptides 4-5 kDa MW, enzymatic digest fragments, natural and synthetic peptides, multi- ring compounds	L1
238TP	C18	5	First generation monomeric C18 phase	Use for same applications as 218TP, but offers different C18 selectivity	L1
201TP	C18	5,10	Non-endcapped C18 phase	Developed for separation of PAHs	L1
202TP	C18	5	Higher carbon load than 201TP	Developed for separation of PAHs	L1
208TP	C8	3, 5, 10	Less hydrophobic than C18TP phase	Polypeptides 10-20 kDa MW	L7
214TP	C4	5, 10	First generation C4 phase	Glycoproteins, haemoglobin variants, histones, insulin variants, membrane proteins	L26
214ATP	C4	5	C4 phase with lower level of endcapping	Optimised for analysis of human growth hormone	L26
219TP	Diphenyl	5,10	Lowest capacity first generation diphenyl phase	hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies	L11

Vydac MS

Vydac MS is a further development of the Vydac range for reversed-phase separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety
of reversed-phases makes this product line suitable for the analysis of small peptides to large intact proteins

Key Features

300 Å pore size spherical silica
Four reversed-phase chemistries
Excellent peak shape with little or no TFA
High protein recoveries make scale-up easy

 

Vydac MS Phase Specification

Phase	Functional Group	Particle Size um	Properties	Application	USP Code
218MS	C18	5, 10	Polymeric bonding, highest hydrophobic interaction and unique geometric selectivity	Use for simple enzymatic digests (<12 proteins) or biomolecules 0–5 kDa MW	L1
238MS	C18	5	Monomeric bonding offers increased peptide interaction and generally yields higher peak counts	Use for same applications as 218MS, but offers different C18 selectivity	L1
208MS	C8	5,10	Lower hydrophobicity is better for larger biomolecules	Ideal for biomolecules 5–10 kDa MW	L7
214MS	C4	5,10	Lower capacity than C18 or C8, suitable for hydrophobic proteins or when minimal organic solvent is desired	Ideal for biomolecules >10 kDa MW, intact proteins, antibodies, oligonucleotides, human growth hormone	L26

Vydac Everest

Everest columns (238EV) have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Leading 300 Å C18−2 chemistries have had carbon coverage in the 2.8 to 3.6 μmol m range. Everest C18 coverage is in excess of 4 μmol m and approximates the theoretical limit based on surface area. The increased shielding of the base silica increases column lifetime and reduces the amount of TFA required to shield the silica.

Key Features

Unique selectivity for hydrophilic and hydrophobic peptides
300 Å pore size spherical silica
Excellent sensitivity with little or no TFA in mobile phase
Ideal for complex enzymatic digests (>12 proteins)

Vydac Denali

Vydac Denali (238DE) is a 120 Å C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes. It has applications for small molecule analyses of interest to pharmaceutical and environmental laboratories

Key Features

High retentiveness
LC/MS of small molecules
Fully scalable from capillary to process

Vydac 302IC

Vydac 302IC is a low capacity anion-exchange (quaternary amine) material based on a high purity 10 μm large pore size silica.

Key Features

• Well referenced for non-suppressed ion chromatography applications
• Used with standard HPLC systems for analysis of common ions
• Widely used for environmental analysis

Select your preferred column from the Online Shop below or send us your specification through the Contact Form