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Vydac HPLC Column

Grace Vydac HPLC Columns

Vydac 300 Å columns are a commonly employed range in bioseparations. Following the acquisition of Grace HPLC ranges by Hichrom, Vydac HPLC  columns continue to be available exclusively from us in capillary to microbore and analytical to preparative dimensions.

Separate biomolecules from small peptides to large intact proteins with the Vydac family of reversed-phase columns (including Vydac TP, MS, Denali, and Everest) and ion exchange (Vydac 302IC) columns. Reversed-phase columns for a polypeptide separation should be considered on the basis of the polypeptide’s hydrophobicity, with molecular weight as a secondary consideration.

Vydac TP

Vydac TP reversed-phase material consists of aliphatic groups bonded to the surface of 300 Å pore diameter silica. The large pores of the TP silica give polypeptide molecules complete access to the interior of the
silica pores. Vydac TP silica is the standard that has defined large pore HPLC for polypeptide separations for nearly two decades.

Key Features

Long column lifetime and negligible phase leaching
Reliable protein purifications, scalable from analytical to preparative scale
Referenced in a large number of patents and publications

Vydac TP Phase Specification

Product groupChemistry Particle Size umPropertiesApplicatonsUSP Code
218TPC183, 5, 10First generation polymeric C18 phase
with unique selectivit
Small polypeptides 4-5 kDa MW, enzymatic digest
fragments, natural and synthetic peptides, multi-
ring compounds
238TPC185First generation monomeric C18 phaseUse for same applications as 218TP, but offers
different C18 selectivity
201TPC185,10Non-endcapped C18 phaseDeveloped for separation of PAHsL1
202TPC185Higher carbon load than 201TPDeveloped for separation of PAHsL1
208TPC83, 5, 10Less hydrophobic than C18TP phasePolypeptides 10-20 kDa MWL7
214TPC45, 10First generation C4 phaseGlycoproteins, haemoglobin variants, histones,
insulin variants, membrane proteins
214ATPC45C4 phase with lower level of
Optimised for analysis of human growth hormoneL26
219TPDiphenyl5,10Lowest capacity first generation
diphenyl phase
hydrophobic proteins, membrane-spanning
peptides, lipid peptides, fusion proteins from inclusion bodies

Vydac MS

Vydac MS is a further development of the Vydac range for reversed-phase separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety
of reversed-phases makes this product line suitable for the analysis of small peptides to large intact proteins

Key Features

300 Å pore size spherical silica
Four reversed-phase chemistries
Excellent peak shape with little or no TFA
High protein recoveries make scale-up easy

Vydac MS Phase Specification

Phase Functional GroupParticle Size umPropertiesApplication USP Code
218MSC185, 10Polymeric bonding, highest hydrophobic interaction
and unique geometric selectivity
Use for simple enzymatic digests (<12 proteins) or
biomolecules 0–5 kDa MW
238MSC185Monomeric bonding offers increased peptide
interaction and generally yields higher peak counts
Use for same applications as 218MS, but offers
different C18 selectivity
208MSC85,10Lower hydrophobicity is better for larger biomolecules Ideal for biomolecules 5–10 kDa MWL7
214MSC45,10Lower capacity than C18 or C8, suitable for
hydrophobic proteins or when minimal organic
solvent is desired
Ideal for biomolecules >10 kDa MW, intact proteins,
antibodies, oligonucleotides, human growth hormone

Vydac Everest

Everest columns (238EV) have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Leading 300 Å C18−2 chemistries have had carbon coverage in the 2.8 to 3.6 μmol m range. Everest C18 coverage is in excess of 4 μmol m and approximates the theoretical limit based on surface area. The increased shielding of the base silica increases column lifetime and reduces the amount of TFA required to shield the silica.

Key Features

Unique selectivity for hydrophilic and hydrophobic peptides
300 Å pore size spherical silica
Excellent sensitivity with little or no TFA in mobile phase
Ideal for complex enzymatic digests (>12 proteins)

Vydac Denali

Vydac Denali (238DE) is a 120 Å C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes. It has applications for small molecule analyses of interest to pharmaceutical and environmental laboratories

Key Features

High retentiveness
LC/MS of small molecules
Fully scalable from capillary to process

Vydac 302IC

Vydac 302IC is a low capacity anion-exchange (quaternary amine) material based on a high purity 10 μm large pore size silica.

Key Features

  • Well referenced for non-suppressed ion chromatography applications
  • Used with standard HPLC systems for analysis of common ions
  • Widely used for environmental analysis


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