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Partisil & Partisphere HPLC Columns

Detailed discription of Partisil and Partisphere HPLC Columns

Historical Evolution Partisil and Partisphere HPLC Columns

Hichrom Limited acquired the complete Partisil and Partisphere HPLC column consumable product lines from Whatman, a division of GE Healthcare, in 2012. Prior to the acquisition Hichrom worked closely with GE Healthcare in the manufacture of these products. Following the acquisition, Hichrom continues to manufacture the full range of genuine columns and media to the same exacting manufacturing protocols and specifications.

Although Partisil and Partisphere products are no longer available from GE Healthcare, the genuine products are still available from the manufacturer Hichrom Limited and Hichrom’s authorised global distributor network.

We are Hichrom Distribution Partner since 1995 and sold distributor for Switzerland

Genuine Partisphere products

Spherical 5um porous silica
Unique void-sealing cartridge hardware
Convenient and easy to use

Partisphere was one of the first commercially available spherical silicas and continues to provide reproducible, high efficiency separations. Partisphere columns are available in a wide range of surface chemistries, including two application specific phases, TAC-1 (Taxane Analysis Column) for the analysis of paclitaxel and other taxanes and WAX (MAX-1) for corn and soy protein analysis.

Partisphere phase specifications

Partisphere Silica
Particle Functional group:
Phase size (um): 5

Partisphere C8
Particle Functional group:Octyl
Phase size (um): 5

Partisphere C18
Particle Functional group:Octadecyl
Phase size (um): 5

Partisphere PAC
Particle Functional group:Amino-cyano
Phase size (um): 5

Partisphere SAX
Particle Functional group:Tetramethyl ammonium
Phase size (um): 5

Partisphere SCX
Particle Functional group:Sulphonic acid
Phase size (um): 5

Partisphere TAC-1 (PFP)
Particle Functional group:Pentafluorophenyl
Phase size (um):5

Partisphere WAX (MAX-
Particle Functional group:proprietary
Phase size (um): 5

Unique Void-sealing cartridge system:

Partisphere columns are available in a unique void sealing (WVS) hardware. If a void eventually forms at the top of the column bed, a simple hand tightening of the inlet fitting moves the frit assembly downwards and recompresses the packed bed, thus removing the void and restoring column efficiency. Large knurled end fittings allow ready hand tightening of the system. All WVS cartridge columns are shipped without end fittings and require end fitting kit, catalogue no. 4631-1001. This item can be interchanged with additional WVS columns.

Genuine Partisil HPLC columns from the manufacturer

Genuine Partisil products
Irregular porous silica
5um and 10um particle sizes
Wide range of column dimensions

Partisil was one of the first commercially available irregular silica’s with a large surface area giving it a high loading capacity. Partisil ion exchange materials are widely referenced and remain one of the most popular choices for analystst. Partisil 10 ODS3 is similar to Waters uBondapak.

Wide range of column dimensions

Partisil phase specifications

Partisil Silica
Phase size (um): 5,10
Particle Functional group: N/A
Description: for normal phase chromatography

Partisil C8
Phase size (um): 5,10
Particle Functional group:Octyl
Description:Partisil C8 is recommended for ion pair chromatography.

Partisil ODS
Phase size (um): 10
Particle Functional group: Octadecyl
Description:Partisil ODS is a low carbon load C18 phase, with intentionally unbonded surface silanols adding to the selectivity.

Partisil ODS2
Phase size (um):10
Particle Functional group:Octadecyl
Description:Partisil ODS2 is a high carbon load C18 bonded silica phase which makes it the most non-polar and, therefore, the most retentive of the Partisil reversed-phase columns. The high sample load capacity is suitable for preparative work.

Partisil ODS3
Phase size (um):5,10*
Particle Functional group:Octadecyl
Description:Partisil ODS3 is an intermediate carbon load C18 phase used for pharmaceutical, natural products, food, biological and environmental pollutants applications. *Partisil 10 ODS3 is similar to Waters uBondapak.

Partisil PAC
Phase size (um): 5.10
Particle Functional group:amino-cyano
Description:Partisil PAC is a Polar Amino Cyano bonded phase with secondary amine groups for good thermal and chemical stability. Fast equilibration enables multiple separation mechanisms, including adsorption, reversed-phase and weak anion-exchange, to be used. The phase is particularly suitable for carbohydrate separations.

Partisil SAX
Phase size (um): 5,10
Particle Functional group:Tetramethyl ammonium
Description:Partisil SAX is a strong anion-exchange phase based on quaternary ammonium groups. It is stable over the pH range 1.5 to 7.5. At intermediate pH, use of a Solvecon eluent conditioning column (see part number 4250-001) is recommended to further enhance column lifetime. Typical applications include nucleic acids, organic acids and inorganic anions.

Partisil SCX
Phase size (um): 5, 10
Particle Functional group:Sulphonic acid
Description:Partisil SCX is a strong cation-exchange phase based on benzenesulphonic acid groups and is stable over the pH range 1.5 to 7.0. At intermediate pH, use of a Solvecon eluent conditioning column (see part number 4250-001) is recommended to further enhance column lifetime. Suitable applications include nucleosides, amino acids, polyamines, drugs and other cationic species.

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