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The leading brand name for Agilent is Zorbax. A number of other brand names are included.
Some columns are packed by Agilent some by Hichrom
The Zorbax product range is structured as follows:
Reversed-Phase Columns
Special Sizes
Rapid Resolution
- Rapid Resolution HD 1.8 µm (1200 bar)
- Rapid Resolution 3.5 µm
- Rapid Resolution HT 1.8 µm (600 b
Special Application
Preparative
- Agilent Preparative
- Preparative Guard Cartridges
- ZORBAX PrepHT
- Dynamax
- Prep to Process: SepTech
- FlowTrap
- Load & Lock
- BioMolecule Purification
Normal-Phase Columns
- ZORBAX NH2
- ZORBAX Eclipse XDB-CN
- ZORBAX CN
- ZORBAX Rx-SIL
Gel Permeation/Size Exclusion
- Full GPC/SEC Portfolio
- Gel Filtration/Size Exclusion
- ZORBAX GF-250/450
- Agilent Bio SEC-5
- Agilent Bio SEC-3
- ProSEC 300S
Ion-Exchange
- Agilent Bio MAb
- Bio-Monolith
- Agilent Bio IEX
- Ion Exchange - SAX and SCX
- PL-SAX PL-SCX
- Hi-Plex for Carbohydrate Analysis
Reversed-Phase for Proteins & Peptides
- ZORBAX 300StableBond
- Poroshell 300
- Capillary/Nano
- ZORBAX 300Extend-C18
- ZORBAX RRHD 300Ĺ 1.8 μm
Zorbax Phases
Zorbax Traditional Columns packed by Hichrom
Features:
- Zorbax spherical porous silica are manufactured from small extremely uniform colloidal silica micro-beads which are agglutinated in a Du Pont patented organic polymerisations process. The organic polymer is then removed by a controlled sintering process to yield mechanically stable silica particles. Zorbax is manufactured by Agilent Technologies Inc in addition to the Hichrom manufactured Zorbax columns. Hichrom continues to offer the traditional range manufactured by Agilent Technologies
- Narrow particle and pore size distribution,
- Robust and reproducible.
- Zorbax columns packed by Hichrom are of top quality
- Column i.d.: Microbore 1 & 2.1, Mediumbore: 3.2, Analytical: 4.0 & 4.6, Semi Prep: 7.75 & 10 and Prep: 21.2mm & 30mm i.d. are available
- Column length: 50, 100, 150, 250mm
- Guard cartridges i various i.d. are available
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Zorbax Hichrom Phase
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Functional Group
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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Silica
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yes
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3,5,7
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70
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330
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TMS
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Methyl
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yes
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5
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70
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330
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4
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C8
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Octyl
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yes
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3,5,7,
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70
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330
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12
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ODS
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Octadecyl
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yes
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3,5,7
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70
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330
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20
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Phenyl
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Phenyl
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yes
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5,7
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70
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330
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12
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NH2
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Amino
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yes
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5,7
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70
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330
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4
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CN
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Cyano
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yes
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3,5,7
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70
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330
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7
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Zorbax Eclipse Plus
Features
- Recommended first choice for method development
- Improved silica and bonding technology
- Extra dense bonding and double capping
- Superior peak shape with basic compounds
- High resolution and efficiency with 1.8, 3.5 and 5 um columns
- Rigorous QA/QC testing
- Wide pH range 2- 9
- Microbore to analytical dimensions
Zorbax Eclipse plus are Agilent Technologies newest range based on improve silica manufacturing and novel bonding technology which Agilent controls from start to finish
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Zorbax Eclipse Plus
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pH rang
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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C8
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2 - 8
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double
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1.8, 3,5,, 5
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5
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160
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6
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C18
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2 - 9
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double
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1.8, 3.5, 5
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95
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160
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8
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PAH
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2 - 9
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double
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1.8, 3.5, 5
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95
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160
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14
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Zorbax Eclipse XBD
Features
- XDB = EXtra Dense Bonding
- Double endcapping through a proprietary process to minimise unwanted interaction between analytes and surface silanols
- Four bonded phases with different selectivity for method development
- Good peakshapes for acids, bases and neutrals
- Wide pH rang 2- 9
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Zorbax Eclips XDB
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pH Range
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Temperature Limit °C
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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C8
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max. 2-9, recom. 3-8
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60
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double
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1.8, 3,5, 5,
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80
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180
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7.6
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C18
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max. 2-9, recom. 3-8
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60
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double
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1.8, 3,5, 5,
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80
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180
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10
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Phenyl
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max. 2-9, recom. 3-8
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60
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double
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1.8, 3,5, 5,
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80
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180
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7.2
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CN
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max. 2-9, recom. 3-8
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60
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double
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1.8, 3,5, 5,
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80
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180
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4.3
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Zorbax Rx and SB (StabileBond)
Features:
- Exceptional stability at low pH
- Stable to pH 6 - best performance below pH 4
- Available as 80A for small molecules and 300A for large molecules
- Non-endcapped
- Nano to prep dimensions available
- Zorbax SB-Aq is an alkyl-bonded StableBond material designed to retain hydrophilic and other compounds when using highly aqueous eluents including 100% water
- Rx for general separations
- Analytical to preparative dimensions
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Zorbax Rx & SB
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Temp Range °C
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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Rx-C8
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80
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no
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3,5, 5
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80
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180
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5.6
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Rx-C18
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60
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no
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3,5, 5
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80
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180
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12
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SB-C3
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80
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no
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3,5, 5 ,
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80
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180
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4
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SB-C8
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80
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no
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3,5, 5
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80
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180
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5.5
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SB-C18
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90
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noi
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3,5, 5
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80
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180
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10
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SB-CN
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80
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no
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3,5, 5
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80
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180
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4
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SB-Phenyl
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80
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no
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3,5, 5
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80
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180
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5.5
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SB-Aq
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80
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no
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3,5, 5
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80
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180
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proprietary
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Zorbax Bonus-RP
Features
- Polar column with embeded amide group in long alkyl chain
- Can be used with 100% aqueous eluent
- Tripple endcapped for good peak shape
- Sterically protected bonding for use at low pH
- Provides different selectivity at low and mid pH
- Microbore to perparative
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Zorbax Bonus RP
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pH range
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Endcapped
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Temperatur limits °C
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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Polar embedded Alkyl
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2 - 9
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triple
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60
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3,5, 5
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80
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180
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9.5
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Zorbax Extend-C18 and 300 Extend C18
Features
- Build on Zorbax Rx-Sil particles
- Designed for high pH applications
- Incorporates a unique C18 - C18 bidenate structure
- Extend C18 for molecules MW< 4000
- 300 Extend for molecules < 40 kDa
- Double endcapping procedures
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Zorbax Extend C18
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pH range
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Endcapped
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Temperatur limits °C
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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Extend C18
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2 - 11.5
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double
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60 at pH < 80, 40°C at pH > 8
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3,5, 5
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80
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180
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12.5
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300 Extend C18
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2 - 11.5
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double
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60 at pH < 80, 40°C at pH > 8
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3,5, 5
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300
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45
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4
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Zorbax Wide Pore 300 SB
Features:
- Specially designed for acidic pH mobile phases used in difficult biological separations such as hydrophobic peptides and proteins
- Exceptional stability at low pH
- Stable to pH 6 - best performance below pH 4
- 300A material for large molecules
- Non-endcapped
- Narrow-bore to semi prep dimensions available
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Zorbax Wide Pore 300SB
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Temp Range °C
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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SB-C3
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80
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no
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3,5, 5 ,
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80
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180
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4
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SB-C8
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80
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no
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3,5, 5
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80
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180
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5.5
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SB-C18
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90
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no
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3,5, 5
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80
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180
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10
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SB-CN
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80
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no
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3,5, 5
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80
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180
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4
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Zorbax Carbohydrae Analysis Column
Features:
- Application specific aminopropyl phase based on Zorbax porous silicate microsphere technology
- Can be used for a wide range of food carbohydrates for regulatory and quality monitoring purposes
- Sugars and sugar alcohols in a variety of food and drink matrices are determined using RI detector
- NH2 Guard cartridges should be used
- Available as 250 mm or 150 mm x 4.6 mm column
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Zorbax Carbohydrate Analysis
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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150 x 4.6 mm
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no
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5
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70
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300
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3.5
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250 x 4.6 mm
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no
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5
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70
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300
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3.5
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Zorbax Poroshell
Features:
- 5 um superficially porous particles
- Fast high resolution separations in proteomics
- Ideal for LC-MS
- Low back pressure
- Manufactured from a thin layer of porous silica on a sold pelicular core of the same material
- The materials are bondet with StableBond 300SB-C18, 300SB-C8 and 300SB- C3 chemistries
- Can be operated a high flow rates to enable peptides and protein to be separated within seconds
- Excellent for LC-MS, column bleed is minimal even at high temperatures
- For analysis of sub-picomole quantities use micro-bore or capillary columns
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Zorbax Poroshell
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Temp Range °C
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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Poroshell 300SB-C3
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80
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no
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5 ,
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300
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Poroshell 300SB-C8
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80
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no
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5
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300
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Poroshell 300SB-C18
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90
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no
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5
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300
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Zorbax Eclipse Amino Acid Analysis (AAA) Column
Features:
- Provides high resolution and rapid separation of 24 amino acids
- Recommended protocol couples this resolving power with the sensitivity and reliability of two well known derivatisation chemistries (OPA and FMOC for primary and secondary amino acids respectively)
- The method can be completely automated for routine analysis and combined with UV or fluorescence detection
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Zorbax Eclipse Amino Acid Analysis Column
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Temp Range °C
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Endcapped
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Particle size um
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Pore Size A
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Surface Area m2/g
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Carbon Load %
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150 x 3.0 mm
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40
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3.5
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75 x 4.6 mm
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40
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3.5
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150 x 4.6
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40
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3.5
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150 x 4.6
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40
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5
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12.5 x 4.6 Guard column
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40
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5
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Zorbax Microbore, Capillary and Nano HPLC Columns
Features
- High resolution columns
- High sensitivity for small samples sizes
- Ideal for LC-MS and LC-MS-MS
- Capillary and nanocolumns for proteomics
- Ideal for low flow rates such as required in particle beam and electrospray LC-MS.
- These columns should be used with appropriate equipment
- Most C8 and C18 Zorbax phase are available in these column configuration
- 1 mm columns are made of Stainless Steel
- 0.3 and 0.5 mm Capillary column are glass lined SS columns
- Trap enrichment columns (5 x 0.3 mm) are made of PEEK
- Nano columns have been developed for Nanoflow LC
- Nano Columns are made of PEEK coated with fused silica of 100 or 75 um id with standard SS end fittings
- Flow rates of 100 nl/min to 1 ul/min are recommended
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