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Zirchrom

Zirconia based materials for unique application

The product range comprises

 

General

  • Porous Zirconia based materials
  • High pH and temperature stability
  • High efficiency
  • Fast solvent equilibration
  • Long column lifetimes

Zirconia bonded phases, manufactured by ZirChrom Separations, Inc. offer unique selectivity relative to conventional silica based columns, combined with extreme chemical and thermal stability. Unlike polymeric phases, zirconia does not shrink or swell as a function of eluent organic content or ionic strength.

ZirChrom Phase

Mode

Particle Size
(μm)

Pore Size
()

Surface Area
(m2/g)

Carbon Load
(%)

pH Range

Temperature
Limit (C)

PBD  

RP

3, 5

300

 30

2.5

1 - 14

150

PS

 RP

3, 5

300

30

1.0

1 - 13

150

EZ   

RP

3, 5

300

30

2.7

1 - 10

50

MS 

RP

3, 5

300

30

3.1

 1 - 10

80

SELECT

RP

3, 5

300

30

 -

1 - 10

80

CARB  

RP

3, 5

300

30

-

1 - 14

150

DiamondBond-C18

RP

3, 5

300

 30

 3.5

 1 - 14

200

PHASE    

NP

3

300

30

 -

1 - 14

150

SAX  

Strong Anion-exchange

3

300

 30

1.2

1 - 12

  80

SHAX

Strong Anion-exchange

3

 300

30

0.9

 1 - 12

80

WAX

Weak Anion-exchange

3

 300

 30

1.2

3 - 9

50

WCX 

Weak Cation-exchange

 3

300

30

 - 

1 - 10

50

PEZ

Cation-exchange

3

300

 30

-

1 - 10

50

Sachtopore-RP13,

 RP

 5

 300

15

-

1 - 12

100

Sachtopore-NP1 

 NP

3, 5

300

 15

-

1 - 14

100

Reverse Phase Materials

ZirChrom-PBD

Is produced by coating ultra-stable zirconia particles with an extremely thin layer of crosslinked polybutadiene. The chemical selectivity and efficiency of ZirChrom-PBD is similar to that of traditional C8 or C18 silica based columns for non-ionic analytes. However ZirChrom-PBD has greater stability than silica phases and higher efficiencies than polymer phases. Selectivity can be modified by the addition of a strong Lewis base, such as fluoride, phosphate or hydroxide, to the eluent. Figure 1 shows the high pH separation of -blockers using a LC-MS friendly amine buffer.

ZIRfig1

 

ZirChrom-EZ

Is a highly efficient phase created by first coating zirconia particles with an extremely thin layer of crosslinked polybutadiene and then deactivating the Lewis acid sites by applying a strong metal chelator. This enables Lewis base analytes such as carboxylates, sulphates and phosphates to be analysed throughout the pH range of 1 - 10 using conventional LC-MS buffers such as acetate or formate.

ZirChrom-MS

Is designed specifically for LC-MS, particularly applications involving basic pharmaceuticals. It has the same deactivation chemistry as ZirChrom-EZ but with a covalent attachment for low bleed and is approximately 2.5 times more retentive for simple compounds. The phase exhibits mixed mode retention characteristics - reversed-phase and cation-exchange.

ZirChrom-PS

Consists of zirconia coated with an extremely thin layer of polystyrene. The phase offers an alternative selectivity and less retention than ZirChrom-PBD making it ideal for non-polar analytes, or where highly aqueous eluents are necessary.

ZirChrom-SELECT

Is a lewis-acid site 'deactivated' carbon-coated zirconia phase. This deactivation reduces solute interactions with the zirconia surface, leading to improved peak shape and wider pharmaceutical compound applicability.

ZirChrom-CARB

Is produced by coating zirconia particles with a thin layer of elemental carbon. It is an alternative to porous graphitic carbon (PGC) supports for the separation of geometrical isomers, diastereomers and other closely related molecules. It offers very different selectivity to ODS or polymeric materials, making it an excellent choice for orthogonal screening in drug discovery.

DiamondBond-C18

Uuses a proprietary covalent bonding technology to graft C18 groups to a carbon-clad zirconia, giving it outstanding stability for LC-MS applications. DiamondBond-C18 has selectivity that is intermediate between ODS silica and unbonded carbon supports for non-electrolytes.

 

Normal Phase Materials

Phase

 

Ion Exchange Materials

ZirChrom-SAX

Iis a highly efficient strong anion-exchanger, created by coating zirconia particles with a thin layer of crosslinked polyethyleneimine. It is useful for the separation of inorganic anions, organic anions and biomolecules such as nucleotides, nucleosides, amino acids and peptides.

ZirChrom-SHAX

Consists of quaternized polyethyleneiminecoated zirconia and is used for strong hydrophilic anion-exchange. It is more hydrophilic than ZirChrom-SAX, making it useful for the anion-exchange of proteins.

ZirChrom-WAX

Is a highly efficient weak anion-exchanger, produced by coating zirconia particles with a thin layer of crosslinked polyethyleneimine. In addition to separating inorganic anions, organic anions and biomolecules, it is an extremely stable amino phase for the separation of carbohydrates.

 

ZirChrom-WCX

Is a phosphate-coated zirconia phase for weak cation-exchange. It is useful for protein chromatography.
 

ZirChrom-PEZ

Is a EDTPA-coated zirconia phase for the cation-exchange chromatography of proteins and the separation of monoclonal antibodies.

 

Sachtopore Phases

  • Highly stable titania based particles
  • Reversed-phase and normal-phase
  • Ideal for preparative scale-up
  • Sachtopore phases from ZirChrom Separations are based on porous, spherical titania (TiO2) particles.

 

RP

NP

Bonded Phase

Polyethylene coated
TiO2

Unbonded
TiO2

Particle Size (μm)

3, 5

 3, 5

Pore Size ()

300

 300

pH Range

 1 - 12

1 - 14

Maximum Temperature

100C

100C

 

Sachtopore-NP (normal-phase)Is bare titania, whereas Sachtopore-RP (reversed-phase) is polyethylene coated titania. This phaseshows similar selectivity to ZirChrom-PBD. Both Sachtopore phases are very chemically and thermally stable and offer unique chromatographic selectivity. Figure 2 shows the temperature stability of Sachtopore-RP at 100C.

ZIRfig2

Whereas reversed-phase titania is useful for the separation of basic pharmaceuticals at high pH, Sachtopore-NP can enablethe separation of isomeric compounds (see Figure 3).

ZIRfig3

Sachtopore can also be used in the selective enrichment and purification of phosphorylated peptides from complex mixtures.

All Sachtopore materials are available in microbore, analytical, semi-preparative and preparative formats and a wide range of particle (3, 5, 10, 20, 40 and 80μm) and pore (60, 100, 300, 1000 and 2000) sizes.

ProTain™ In-Line Protein Removal

ProTainTM is a system for the effective in-line removal of matrix proteins from samples. It is based on a 3μm polymer-coated zirconia material which acts as a protein sponge, allowing small molecules of interest to pass through the analytical column. ProTain can be used in-line with any type of silica, polymer or zirconia-based analytical column

 

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Our Company Profile and Summary Product List 2015
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