Gift  for Enduser
A limited number of Leathermans will be offered for any order over CHF. 3000.-. from end-user. This offer is valid until 30^th April 2012.

Geschenk an Chromatographen

Wir offerieren eine beschrankte Anzahl von Leadermans für Bestellung von > CHF 3000.- bis zum 30. April  2012

Welch Material Inc. manufactures a competitive range of popular HPLC columns.

The company was founded 2003 in Maryland,USA and Shanghai, China by several Ph.D. scientists from top Universities such as MIT and University of California at Berkeley. They have extensive experience in particle surface modification and bonding chemistry.  Through optimal utilization across two countries, they have developed many innovative  LC and GC consumable products, in particular HPLC and SPE products. Welch Materials is a fast growing company with sales in China, USA, India, Brazil, Canada and Europe.

Attention We represent Welch Materials for Europe. For general enquiries please click here

• Asian Clients please contact www.welchmat.com  or e-mail: huiyang at welchmat.com
• American Clients  please contact www.welchmat.com/english

The product range is structured as follows:

• Ultisil XB Range made from spherical, totally porous, and ultra high purity (>99.999%) type B silica particles.  Analytical and prep column
• Ultisil XB Reverse Columns (C18, C8, C4, C1, Phenyl)
• Ultisil XB Polar bonded Columns (CN, Si2O, NH2, Diol)
• Ion Exchanger Columns (SAX, SCX)
• Chiral Columns (Chiral AD, AS,  OD and AJ)
• Polar Embedded HPLC Columns (Polar RP, Phenyl-Ether)
• Fluorinated Columns (PF-Phenyl, F-C8)
• Analyte Specific Columns (PAH, Amino Acid, C30)
• Unique Columns (LP-C18, AQ-C18)
• Ultisil UHPLC Range
  Available in C18, Phenyl, Polar RP, and HILIC
• Topsil Product Range (silica based analytical and prep column with the same surface chemistry as Ultisil XB but with significantly different selectivity.)
• Xtimate hybride and polymer Range of HPLC Columns
• Xtimate Hybride Reverse Columns:(C18, C8, C4)
• Xtimate Phenyl-Hexyl Columns
• Xtimate CN Columns
• Xtimate Polar RP
• Xtimate Saccharide-Columns :
• Welchrom SFE materials

Comnments from the Market

Large Multinational Agrochemical Enterprise My colleague is thrilled with the quality of Ultisil Column / Mein Kollege ist begeister von der Ultisil-Säule ... Research Department in Large University We compared Ultisil against a number of heavily promoted brands. Ultisil has the very best price/performance ratio.

Ultisil^® HPLC columns are made from spherical, totally porous, and ultra high purity (>99.999%) type B silica particles. Our proprietary surface modification treatment before bonding generates a very smooth and uniform surface with less acidic surface silanols.

Welch offers a full line of phases including XB-C18, AQ-C18, XB-C8, XB-Phenyl, and XB-CN to provide a wide range of selectivities. These phases were developed using state-of-the-art bonding chemistry technology to provide unique performance characteristics to enable the most demanding separations. All phases are fully end-capped by proprietary techniques to ensure excellent peak shapes and long lifetime. All bonding, end-capping and packing processes are optimized and well-controlled to achieve maximum efficiency, excellent batch-to-batch and column-to-column reproducibility, and longer lifetime. Ultisil^® HPLC columns provide the Ultisil performance in peak shape, efficiency and resolution. Through continuous innovation, Welch Materials offers customers the best tools and solutions for separation, purification, and analysis of organic and bioorganic compounds.

Application Data Index (Find your Application of interest and ask forappropriate  chromatograms)

Welch_Ultisil_Index_of_Comparison_and_Performance_Data

Ultisil XB Reverse Phase Columns

Ultisil XB - C18 :

• The most universal C18 column featuring high surface coverage and exhaustive double endcapping providing optimal separation for basic, neutral, polar and acidic molecules. 
• Product is sold in mid price range

Ultisil XB-C8 :

• Less retention than with C18 ligands
• Selectivity choices for method development optimization
• Good peakshape for basic, acidic and neutral compounds
• High performance over a wide pH range
• Particle size from 3 to 10 um
• Long life with extra dense bonding and double endcapping

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ XB-C8 Octyl double 3,5,10 120'200'300 320 1.5-10 12

Ultisil XB-Phenyl :

• Different selectivity than alkyl phases C18, C8 and CN
• Excellent for aromatic, polar compounds and difficult pharmaceuticals
• High surface coverage  and exhaustive double end capping
• Exceptional batch - to-batch and column to column reproducibility
• Available in dimensions from analytical to preparative for all sample sizes

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ XB-Phenyl Phenyl double 3,5,10 120'200'300 320 1.5-10 13

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Polar bonded Phase

Ultisil XB-CN :

• Low hydrophobicity, for rapid elution of hydrophobic molecules
• Suitable for use both in Reverse and Normal phase mode.
• Unique selectivity for polar compounds
• Retention and separation of strongly basic analytes, including quarternary ammonium salts with excellent peak shape
• Compatibility with highly aqueous mobile phases
• Exceptional batch to batch and column to column reproducibility
• Exceptional stability and column life-time, from pH 1.5 to 9.0
• Available in dimensions from analytical to preparative for all sample sizes

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ XB-CN Cyano double 3,5,10 120'200'300 320 1.5-9 7

Ultisil XB-SiO2 :

• A Normal phase material made from spherical, ultra high purity Type B silica
• Just like XB-CN can be used in in Normal and  Reverse phase modus
• Achieved goo results for analysis of polar compounds which always lead to peak tailing

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ XB-SiO2   no 3,5,10 120'200'300 320 2-8.0 0

Ultisil XB-NH2 :

• Column is used in Normal phase mostly
• Propyl-amino silane phase bonded to Ultisil Silica
• Used for normal phase and weak anion-exchange  and reverse phase HPLC of polar compounds
• For applications in aggressive normal phase mode with aqueous eluent
• Vitamin A and D are separated in the normal phase mode  and carbohydrates  and sugars are separated in reverse phase mode

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ XB-NH2 Amino no 3,5,10 120'200'300 320 2-8.0 6

Ultisil XB-Diol :

• May be used as polar sorbent in normal phase mode
• Like bare silica XB-Diol has the ability to form hydrogen  bonds and to separate structure isomers
• Since most of its surface is covered with organic functions Ultisil XB-Diol adsorbs less water, with less or more reproducibility
• More stable than the traditional normal phase  column, such as silica or amine
• Could be used in reverse phase
• Similar polarity to amine
• Good selectivity without excessive retention
• Improved peak shape vs. bare sillica

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ XB-Diol Diol no 3,5,10 120'200'300 320 2-8.0 8

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Ion Exchanger Columns

Ultisil SAX :

• Silica based polar linked strong anion exchanger
• High resolution because of silica structure and high efficiency
• Polar bonded phase consisting of ammonium-functionalised  silane
• Organic modifiers such as acetonitrile  and methanol may be used with SAX columns within organic/buffer solubility constraints
• Retention can be controlled by varying pH, ionic strength  and organic modifier content
• Stable from pH 2 to 7

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ SAX Ammonium fuctionalize d silane   3,5,10 120'200'300 320 2-7.0

Ultisil SCX :

• Strong cation exchanger with covalently bonded sulfonic acid moiety
• High resolution because of silica structure and high efficiency
• Polar bonded phase consisting of ammonium-functionalised  silane
• Organic modifiers such as acetonitrile  and methanol may be used with SAX columns within organic / buffer solubility constraints
• Retention can be controlled by varying pH, ionic strength  and organic modifier content
• Stable from pH 2 to 7

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ SCX aromatic sulphonic acid  moiety   3,5,10 120'200'300 320 2-6.5

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Chiral Phases

Ultisil Chiral  :

• There are four types of chiral phases
• 1. Chiral AD,
• 2. Chrial AS,
• 3. Chiral OD
• 4. Chiral AJ
• Based on polymer coated silica
• 80% of all racemate compounds can be separated by those four columns
• Chiral AD and Chiral OD is the most used chiral column
• Excellent resolution of racemats
• Fast, easy method  development
• Columns are durable and long lasting
• Smooth transition from lab to development, pilot and production scale.

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ Chiral   / 5,10 120 / 2.0-9.0 /

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Polar Embedded HPLC Columns

Ultisil Polar-Embedded RP :

• Polar embedded silica.
• Excellent selectivity for polar molecules
• Polar embeded phases have been developed for more than 10 years. Early developments where amide phases  with problem of phase collaps.
• Excellent peak shape for polar compounds

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ Polar-RP   double 3,5,10 120'200'300 320 1.5-10 18

Ultisil Phenyl-Ether :

• As with RP but with C 18 RP
• Polar embedded silica with phenyl ether groups

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ Phenyl-Ether   double 3,5,10 120'200'300 320 1.5-10 13

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Fluorinated Phases

Ultisil PF-Phenyl :

• Primarily used for separating fluor containing molecules
• Ideal for separating fluor containing aromatic molecules.
• May also be used for separating non fluorous compounds such as Taxol, and its derivatives because of its aromatic ring it has a higher selectivity compared to other alkyl-fluoronated phases
• Can separate nitro-benzene isomers (para vs. ortho) which can not be separated by traditional phenyl phase

Ultisil F-C8 :

• High  selectivity and retention towards halogenated aromatics and alkyl compounds
• Just different from octal alkyl phase

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Analyte Specific Columns

Ultisil PAH :

• Silica based column for separating Polycyclic Aromatic Hydrocarbon.
• Optimised for EPA Method 610 in less than 30 min. with high resolution
• Very good peak shape

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ PAH   no 3,5,10 120'200'300 320 1.5-10

Ultisil Amino Acid :

• Made from spherical totally porous and ultra high purity type B silica particles
• Proprietary bonding method applied to create smooth surface with less acidic silanol groups on the surface
• Provide the best performance in peak shape , efficiency  and resolution for the analysis of 18 Amino acids within 30 min.
• Most amino acids have no absorbance under UV detectior, the sample should be derived before HPLC  analysis
• The package includes:
• One Amino acid HPLC column
• Amino acid  bench mark (18 kinds of amino acids)
• Reagent for derivatisation
• Amino acid analysis user manual
• Download PTIC Method

Ultisil C30 :

• Compared to classical C18 stationary phases, C30 is much more hydrophobic and retainable. Even when pure organic eluents is applied, many sample solutes, for example  carotenoids are able to be retained.
• Ultisil C30 is designed for the separation of geometric isomer recognition, polar carotenes, polar and non polar xanthophylls, steroids, retinols and fat soluble Vitamins (A, D, K and E)
• Comprises polymeric C30 alkyl chains
• Very lipophylic
• Exceptionally selective pattern for geometric isomers

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ C30           2-6.0

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Unique Phases

Ultisil LP - C18 :

• The column for low pH application (pH 1)
• Comprises of two, very bulky hydrophobic protective groups to prevent siloxane bond from hydrolysis at low pH condition.
• For use with very low pH eluents (pH 1.0) at high temperature
• Is the most stable C18 low pH material in the market
• Because LP is not endcapped and has more surface silanols LP phase has more retention for some early eluted polar compounds and provides some different selectivity than traditional C18 phases.
• LP-C18 is the most polar C18 amongst all of the C18  phases
• Endures 100% water as the mobile phase
• Best peak fro polar compound analysis
• Exceptional lifetime at low pH (1.0 - 8.0)

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ LP-C18 Octadecyl no 3,5,10 120'200'300 320 1-8.0 10

Ultisil AQ-C18 :

• Reverse Phase material with high selectivity for polar and hydrophilicity, which are either not or poorly retained on other phase.
• A proprietary bonding chemistry avoids phase collaps, even if 100% water is being used.
• AQ-C18 is fully endcapped to ensure best peak shapes of polar and basic compounds and longer lifetime.
• Typical applications are separations of biomolecules, metabolites and pharmaceutical degradants such as organic acids , water soluble vitamins, oligosaccarides , amino acid,  small peptides and nucleotides

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Ultisil™ AQ-C18 Octadecyl double 3,5,10 120'200'300 320 1.5-10 12

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Ultisil UHPLC Columns

1,8 um Particle size

Ultisil UHPLC C18

Ultisil UHPLC Phenyl,

Ultisil UHPLC Polar RP,

Ultisil UHPLC  HILIC

please enquire

Xtimate Hybride Reverse Phases/Columns:

Xtimates are Welch premium HPLC column series. There are two types of Xtimate column, those based on based on Hybride Silica Polymer packing materials, and those based on pure polymer materials.

Xtimate C18 :  Silica coated with 2 nm silane polymer coating and C18 alkyl groups. High mechanical stability

Test condition:

• Column:            Xtimate C18, 5um,150 x 4.6 mm / Gemini, C18, 5 um, 150 x 4.6 mm
• Mobile Phase:  A: 10 mM Ammonium Bicarbonate pH 10.5
                             B: 90 :10 Acetonitrile / buffer
• Gradient:           0% to 100% B in 10 min.
                            100% B last 7 min.
                             0% B last 3 min.
• Flow:                  1.0 mL/min
• Temp:                50°C
• Detector:           UV @ 254 nm

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ C18 Octadecyl yes 3,5,10 120'200'300 320 1-12.5 14

Xtimate C8 :

• Silica hybride with C8 alkylgroups

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ C8 Octyl yes 3,5,10 120'200'300 320 1-12.5 10

Xtimate C4 :

• Silica hybride with C4 alkylgroups

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ C4   yes 3,5,10 120'200'300 320 1-12.5

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Xtimate Phenyl-Hexyl :

• Silica hybride with phenyl - hydride moieties

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ Phenyl-Hexyl Phenyl-Hexyl yes 3,5,10 120'200'300 320 1-12.5

Xtimate CN :

• Silica hybride with CN groups

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ CN Cyano yes 3,5,10 120'200'300 320 1-12.5

Xtimate Polar RP: 

• Silicahybride with embedded polar reverse phase groups.

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ Polar RP     3,5,10 120'200'300 320 1-12.5

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Xtimate Saccharide-H :

• Packed with spherical, low cross linked polystyrene/divinylbenzene PS/DVB  particles of  5 um and 8 um size. The surfaces are modified with sulfonic acid (-SO3H). The separating mechanism is based on ion-exchange mechanisms.
• Compatible with most aqueous mobile phases, including pure water as eluent. Wide operating temperature 20 - 90°C. pH range 1-3 . Analytical and preparative columns.
• Used for Ribavirin detection. Ribavirin is an anti-viral drug used by many hospitals  in the treatment of respiratory syncytial virus infection

Column Functional Group Endcapped Particle size um Pore Size A Surface Area m2/g pH Stability Carbon Load % Xtimate™ Saccharide-H     6,8 no pore     Crossing Link 10%

Xtimate Saccharide-Ca :

• Packed with spherical, low crosslinked polystyrene/divinylbenzene PS/DVB  particles of  5 um and 8 um size. The surfaces are modified with sulfonic acid (-SO3H ,followed by chelating of calcium ions (Ca2+). The separating mechanism is based on ionexchange mechanisms.
• Compatible with most aqueous mobile phases, including pure water as eluent. Wide operating temperature 20 - 90°C. pH range 1-3 . Analytical and preparative columns.
• Used for analysis of sugar products such as hydrolysis of beet, cane and starch in streams processing plants . Glucose, fructose, maltose and maltotriose can be separated from higher oligomers found in typical corn syrups.al virus infection

Welchrom see under Sample Preparation