SieLC was formed by two highly innovative chemists Vlad Orlovsky and  Yuri Zelechonok in Prospect Hights, Il, north of Chicago.

Their Product range is structured as follows:

• Primesep : Mixed mode range of column
• Primesep Specialties and Method Development Kits
• Obelisc : Mixed Modes Columns based on Living Separation Cell Technology
• Promix : A novel approach to separate proteins and peptide

Application List

Primesep Columns

Summary

• Novel silica based mixed mode phases
• Suitable for RP, NP, Ion-Exchange  and Ion-Exclusion Chromatography
• Unique adjustable selectivity
• Stable in 100% aqueous eluents
• LC-MS and preparative chromatography application

Introduction

Primesep mixed mode stationary phases have been developed by SIELC TECHNOLOGIES for separating a wide range of polar and non-polar compounds by different separation modes, based only on eluent selection. Ionizable compounds interact with the stationary phase by reverse phase, ion-exchange or ion exclusion mechanisms

Each Primesep column has dual chemistry stationary phase  containing a long hydrophobic alkyl chain and an ionizable cationic or anionic embedded group. Reverse phase and ion-exchange interaction can be controlled independently ; reverse phase by organic concentration and ion-exchange by eluent ionic strength and pH. All columns offer the same hydrophobic retention properties but differ in their ion-exchange properties.

Primesep columns are suitable for analytical and preparative scale separations in isocratic and gradient modes, and are compatible with common detection techniques.

These columns are resistant to dewetting in 100% aqueous eluent and are stabile in pure organic and highly acidic conditions  down to pH 1.0. They can efficiently separate organic and inorganic ions on the same columns at the same time. This enable a organic pharmaceutical to be quantified simultaneously with with its inorganic counter ions. Also, iorganic cations and anions can be run together without a specialised ion chromatography system.

The choice of buffer for use with Primesep columns depends on the detection technique. For UV detection, TFA, sulfuric acid, phosphoric acid and their salts are recommended. For LC-MS or ELSD detection, the best choice is TFA, ammonium formate, ammonium acetate, formic and acetic acid.

Retention of Polar Compounds

Primesep Literature for download

SIELC_Primesep_Product_Catalog

SIELC_Primesep_ An_alternative_to_IC

SIELC_Pharma_Problem_Solving

Generic screening method for Primesep 200 column - UV

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Embedded Acidic Primesep Phases for cation exchange

SIELC Technologies provides  several different columns with negatively charged functional groups due  to the embedded anionic ion-paring reagent.

Primesep A is the strongest acidic column, while Primesep C is the weakest acidic column. The Primesep 100 and Primesep 200 columns are in between. Difference in the functional group acidity allows selecting the most appropriate column for a particular set of basic compounds that differ in their pKa value.

The embedded acidic functional group can be in an ionized form, or in a non-ionized form, depending on the pH of the mobile phase. In order to get retention by ion-exchange mechanism on Primesep® columns, the pH of the mobile phase should be selected close to, or above, the transition value of embedded acidic groups. Below the transition pH value, the column behaves as a regular reverse-phase column with an embedded non-ionized polar group.

The column name corresponds to pH value of the mobile phase where acid residue on the column switches from ionize form to neutral one.

Primesep A is a reverse-phase analytical column with embedded strong acidic ion-pairing groups.

• Improves retention of weak basic compounds by cation-exchange mechanism.
• Separates acids by ion-exclusion mechanism.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep A columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep 100 is a reverse-phase analytical column with embedded acidic ion-pairing groups.

• This is the most versatile column of the entire Primesep family for separation of a broad range of compounds.
• Improves retention of basic compounds by cation-exchange mechanism.
• Separates acids by ion-exclusion mechanism.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 100 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep 200 is a reverse-phase analytical column with embedded weak acidic ion-pairing groups.

• Improves retention of strong basic compounds by cation-exchange mechanism.
• Retains neutral compounds by reverse-phase mechanism.
• Provides an additional interaction with polar molecules offering separation of isomers and structurally-related compounds.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 200 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Amino Acid Separation by BLIS Technology

Primesep 500 is a reverse-phase analytical column with embedded acidic ion-pairing groups.

• Primesep 500 has an embedded carboxilic acid with a pKa of about 5.
• Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
• Separates acids by ion-exclusion mechanism at a mobile phase pH > 5.
• Retains basic compounds, as any RP column, at pH < 5
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 500 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Versatility of Primesep Stationary Phases

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Primesep C a reverse-phase analytical column with embedded complex-forming groups.

• Improves retention of positively charged compounds by forming a host-guest complex.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep C columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Complex Interaction with Primesep C:

SIELC Technologies has developed a new class of columns with combined reverse-phase, cation  exchange, and embedded complex-formation (carboxylic acid) properties in interaction with amines,  sulphonium, phosphonium and metal ions.

This column has a unique unparallel selectivity for complex mixtures separation.

The pH working range  for these columns is from 1 to 7, but their complex formation and  cation-exchange properties are substantially suppressed at the pH below  3. In order to facilitate the complex formation, the pH of the mobile  phase should remain in the range of 3-7. The degree of complex formation can be adjusted by selecting the pH of the mobile phase.

The unique complex properties of the  column can be observed in the separation of alkali metals ions. The ions elute on the Primesep C column in reverse order compared to  ion-exchange elution e.g. Li+ > Na+ > K+.. The unusual elution order is found in primary,  secondary, and tertiary amines. Secondary and tertiary amines have  higher pKa values than primary amines, which makes them retain longer by ion-exchange mechanism.

With the complex forming  properties of Primesep C columns, the primary amines are more retainable than secondary and tertiary amines. This extraordinary elution pattern  allows tailoring the separation of amines to provide the most  appropriate peak order for quantitation and preparative chromatography.

Primesep P a reverse-phase analytical column with embedded aromatic and acidic ion-pairing groups.

• Improves retention of basic compounds by cation-exchange mechanism.
• Separates aromatic compounds by difference in pi-pi interaction.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep P columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

HPLC separation of aromatic compounds on Primesep P columns.

SIELC Technologies has developed a new class of stationary phases, which provides three interactions with analytes:

    reverse-phase interaction
    pi-pi interaction
    strong cation exchange interaction
Depending on analyte properties, one, two, or all three interactions can be applied to the separation

This stationary phase facilitates the separation of structural isomers of aromatic compounds.

Additional pi-pi interaction creates a difference in the bind state for structural isomers and often resolves critical pairs of compounds. The degree of pi-pi interaction can be adjusted by varying the amount of acetonitrile in the mobile phase

When methanol is used as an organic modifier, the highest degree of aromatic interaction can be achieved.

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Embedded Basic Phases  for Anion Exchange operation

Primesep B Type of columns

SIELC Technologies provides 3 different B-type columns. Primesep B, B2 and SB phases are produced with embedded basic iron-pairing groups. In addition to improving  the retention of acidic compounds by anion-exchange, the phases separate  bases by an ion-exclusion mechanism.

Primesep SB is a strong basic column. The recommended pH range is from 1.5 to 4 created by the addition of trifluoroacetic, phosphoric or perchloric, or formic acids to the mobile phase.

Primesep B2 is a weak basic column. It also has carboxilic acidic functional groups. At pH of the mobile phase below 5 the acidic groups are not ionized and B2 column surface becomes positively charged. This dual chemistry offers extended pH range from 0.5 to 7, suitable for the ammonium acetate and ammonium formate buffered mobile phases.

Primesep D column originally developed for direct plasma analysis, became very useful for other anion-exchange / reverse phase applications. Primesep D comprises an anion-exchange group embedded  in a long alkyl chain. It is a stronger basic column than Primesep B2. Primesep D allow direct injection of plasma and other biofluids, enabling a broad range of small  molecules to be analysed by a single column. It has extended pH range from 1.5 to 7 and offers similar properties as Primesep B2 column but the column has no carboxilic groups and remains positively charged throughout the working pH range.

Embedded Acidic and Basis Phase

Primesep AB column is a zwitterionic, reversed phase column with embedded cation exchange and anion exchange functionalities, combining the properties of both in a single column.. This column is able to separate  complex mixtures of polar anionic and cationic compounds.

All B-type columns provide at least two main interactions with analytes: the reverse-phase interaction and the anion-exchange interaction. Neutral analytes retained by reverse-phase interaction. The presence of the charged group in the alkyl chain provides additional selectivity uncommon for typical reverse-phase columns. Acidic analytes can be retained by both anion-exchange and reverse-phase mechanisms. To control retention and selectivity, there is a broad selection of the mobile phases with concentration of organic modifier in the 0-100% range. The concentration and the type of an acid will also significantly affect the retention of anionic compounds. Basic compounds can be retained only by the reverse-phase mechanism, but the presence of positively charged groups on both analyte and stationary phases produces the unique selectivity due to the ion-exclusion phenomena.

Primesep B columns retain acid residue in the stationary phase in equimolar amount. When a column switches to the mobile phase with another type of acid, it should be sufficiently equilibrated to replace all counter-ions from the previous mobile phase with new counter-ion.

Direct Plasma Analysis with Primesep D

Primesep SB is a reverse-phase analytical column with strong embedded basic ion-pairing groups.

• Improves retention of acidic compounds by anion-exchange mechanism.
• Separates bases by ion-exclusion mechanism.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5).

Primesep SB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep B2 is a reverse-phase analytical column with embedded basic ion-pairing groups.

• Improves retention of acidic compounds by anion-exchange mechanism.
• Separates bases by ion-exclusion mechanism.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 7).

Primesep B2 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep D is a  reverse-phase analytical column with embedded basic ion-pairing groups  specifically design for direct plasma analysis.

Does not retain high-molecular weight plasma components.

• Retains hydrophobic and acidic components.
• All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4).

Primesep D columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep AB is a reverse-phase column with embedded acidic and basic ion-pairing groups.

• Improves retention of strong acidic compounds and strong basic compounds by ion-exchange mechanism.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep AB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

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Primesep N is a normal-phase analytical column with embedded acidic groups.

• Primesep N has embedded acidic groups with a pKa of about 5.
• Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
• Separates polar compounds by HILIC mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 300 A, 100 A

Primesep S2 is a silica-based acidic-column with hydrophilic properties.

• Separates basic compounds by cation-exchange mechanism.
• Separates basic and neutral polar compounds by HILIC mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep S2 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores:100 A

Primesep B4 HPLC Columns for Separating inorganic anions

Primesep B4 is a reverse-phase (short carbon chain) analytical column with embedded basic ion-pairing groups.

• Improves retention of acidic compounds by anion-exchange mechanism.
• Retains and separates basic and acidic surfactants based on multi-mode mechanism.
• Separates hydrophobic bases by ion-exclusion mechanism.
• Retains hydrophobic neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 4.5).

Primesep B4 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep PB HPLC Columns

Primesep PB is a reverse-phase analytical column with embedded aromatic and basic ion-pairing groups.

• HILIC Retention of Polar Compounds
• Improves retention of acidic compounds by anion-exchange mechanism.
• Separates aromatic compounds by difference in pi-pi interaction.
• Retains neutral compounds by reverse-phase mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 5).

Primesep PB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep S HPLC Columns

Primesep S is a normal-phase analytical column with embedded acidic ion-pairing groups

• Most suitable for separation of common sugars and other neutral or very polar molecules.
• Retains and separates amino acids with different buffers at low concentration.
• Separates weak bases by ion-exchange mechanism.
• Separate acids by ion-exclusion mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 7.0).

Primesep S columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep AP HPLC Columns

Primesep AP is a silica-based amino-column with hydrophilic properties.

• Separates acidic compounds by anion-exchange mechanism.
• Separates basic and neutral polar compounds by HILIC mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep AP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep X HPLC Columns

Primesep X is a silica-based amino-column with weak hydrophobic properties.

• Separates acidic compounds by anion-exchange mechanism.
• Separates basic and neutral compounds by RP mechanism.
• All mobile phases are LC-MS and preparative chromatography compatible.

Primesep X columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths:250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles:10 um, 5 um
Pores: 100 A

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Mixed Mode Method Development Kits

As an aid to method development, kits containing 3,4 or 5  columns of the same dimensions can be supplied .  These phases can be customer selected to be most appropriate for the required application

• Most Popular Kit: Primesep 100, B2, and C
• Most diverse Kit: Primesep 100, B2, C and 200

Please contact us for information

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Obelisc Columns

Overview:

• Multi separation modes
• Simple eluent selection
• MS, ELSD and low UV (<220 nm) compatible
• Adjustable selectivity

With the increasing demand for the analysis of polar and ionisable  compounds, SIELC Technologies have developed Obelisc - a new  generation of mixed mode columns. Obelisc R and Obelisc N are complimentary phases for the separation  of polar and non-polar compounds using multiple separation  mechanisms (RP, ion-exchange, NP, ion exclusion). Both phases have  positive and negative charges separated by a long organic chain,  allowing both charges to simultaneously participate in electrostatic

SIELC_Obelisc_Intro_Brochure

Compound / Phase Interaction Mechanisms

Two complimentary columns, Obelisc R and Obelisc N, based on Liquid Separation Cell ( LiSC ) technology, offer a new approach to separate a variety of small molecules. Buffer concentration, buffer pH, and organic modifier concentration are three orthogonal parameters of the mobile phase that allow adjustment of column properties to separate complex mixtures.

Obelisc N is a column which has very polar characteristics and works well for polar and charged analytes. It has anions closed to the surface separated from the cationic  groups by a hydrophilic chain

• Obelisc N offers both positively and negatively charged groups to interact with positively or negatively charged analytes.
• Multiple separation modes (NP, HILIC, IE).
• In HILIC mode, Obelisc N offers different selectivity form other HILIC or bare silica columns.
• Mass spec, ELSD, preparative and low UV (<220 nm) compatible, low concentration buffers.

Obelisc R is a reverse-phase analytical column and can be used in traditional, reversed-phase type applications.

• Due to the presence of ionic groups and a long hydrophobic chain,  Obelisc R offers additional retention and tuning that is not available  with traditional reversed-phase columns.
• Multiple separation modes (RP, NP, IE).
• MS, ELSD, CAD, preparative and low UV (<220 nm) compatible, low concentration buffers.

Specially developed “AQ” type column are designed to work in low organic containing eluents, often 100% aqueous for the analysis of polar compounds by reverse-phase. However they can often offer insufficient improvement in retention of highly polar molecules. Obelisc R can be used in traditional RP type applications and it offers  and it offers significant improvement in the degree of retention of both acidic and bsic polar molecules, compared with “AQ” type phases

Obelisc R & N  HPLC Columns

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Promix  for biomolecule separation

Overview

• Peptide and Protein separation
• 2D HPLC with a single column
• Alternative selectivity  to reversed-phase  300 A columns
• Scalable from capillary to preparative

Promix is an alternative chromatography technology for efficient resolution of peptides and proteins. The technology is based on a combination of two interactions - hydrophobic and ionic. This approach is possible due to a new type of separation media: a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support. With this phase, unparalleled selectivity and peak capacity can be achieved. Independent adjustment of the amount of buffer and organic modifier creates an infinite number of separation conditions that are suitable for many types of biomolecules.

Proteins and Peptides on Promix Columns

There are four different Promix Columns to be selected as follows:

Use the following chart to select a column based on the analyte's properties

 Select the column length based on sample complexity

• 150-250 mm for proteomics, protein digests, and complex samples
• 50-150 mm for synthetic analysis and purification

Select the column i.d. based on sample loading and sensitivity needs

Mobile Phase should be pH 2-4 with:

• TFA (0.05-0.3%)
• Formic Acid (0.1-0.9%)
• Ammonium Acetate or Ammonium Formate
• Organic Modifier should be Acetonitrile
• Ionic strength, pH, and organic modifier gradients can be used to optimize the separation

Promix SP HPLC Columns

Promix SP is a stationary phase for small peptides under 1 kDa with PI value below 6.0.

• Reverse phase and ion-exchange mechanism of interaction.
• Use acidic mobile phase pH from 2 to 5.5.
• Use ion strength at least 20 mM.
• 100Ã… pore size is available.
• Promix SP is a column for very short and/or very hydrophilic peptides. Typically it is difficult to retain such peptides on RP column. The Promix SP column offers an improved retention profile and alternative selectivity if compared with RP separation.

Promix SP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A

Promix AP HPLC Columns

Promix AP is a stationary phase for small and medium size peptides under 3 kDa with PI value below 7.0.

• Reverse phase and ion-exchange mechanism of interaction.
• Use acidic mobile phase pH from 2 to 5.5.
• Use ion strength at least 20 mM.
• 100Ã… and 300Ã… pore sizes are available.
• Promix AP is a column designed for short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides on this column. Use a buffer with a neutral or slightly acidic pH (4.5 - 7) in the beginning of the gradient, and use a more acidic buffer (pH 2 - 3.5) in the end of the gradient elution.

Promix AP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 300 A, 100 A

Promix MP HPLC Columns

Promix MP is a stationary phase for medium size proteins and peptides from 1 to 10 kDa.

• Broad pI value.
• Reverse phase and ion-exclusion mechanism of interaction.
• Use acidic mobile phase pH from 2 to 5.5.
• Use ion strength at least 1 mM.
• 300Ã… and 800Ã… pore sizes are available.
• Promix MP is a column designed for medium size hydrophobic peptides. Typical applications include protein digest which increases peak capacity of the digest by 30 to 50%. This column offers alternative selectivity if compared with simple RP columns. Typically difficult to separate pairs of peptides can be resolved on this stationary phase.

Promix MP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 800 A, 300 A

Alternative Selectivity of Promix

Peptide Digest Separation with Promix MP

Peptides Analytic with Promix

Peptides Different Gradient Types with Promix MP

Separation of Insulin Analogues With Promix MP

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