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QuEChERS  kits

QuEChERS – a Simplified very successful, popular Method of Sample Preparation for Pesticide Analysis

Product Catalogue

History

In 2003, M Anastassisdes developed a simple way to prepare food samples for pesticide analysis. It’s called QuEChERS (pronounced as “Catchers”), an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe. With QuEChERS, you could prepare your samples in multi-class, multi-residue pesticide analysis while saving time and money. Welch Materials provides high quality, competitive priced Welchrom® QuEChERS for your sample preparation.

Why Welchrom® QuEChERS?

  1. Saving time and money for your sample preparation
  2. High quality, best reproducibility
  3. Lowest contamination
  4. Lower cost as compared to other brands

How QuEChERS works?

QuEChERS extraction method is designed for multi-residue pesticide analysis of fruits and vegetables with high water content (80%-95%). Some compounds will interfere the detection of target pesticide, such as chlorophyll, fatty acid, pigment, which could be removed by QuEChERS. QuEChERS can be performed by three different methods, The AOAC 2007.01 Method, the EN 15662 Method or the Original Non-Buffered Method.

Wellchrom Evolution of Quetchers

 

Welchrom® QuEChERS Workflow

Step 1: Extraction

Transfer homogenized sample (10g or 15g) to 50ml tube, then add extraction solvent and salt, shake for one minute, centrifuge > 1500 rcf for 1 minute.

  • Welchrom® QuEChERS Extraction Kits: 50ml tubes Pre-packaged MgSO4, NaCl or other salts

Welchrom® QuEChERS Extraction Bags Ordering Information (without 50ml tubes)

 

Part Numbers

Pack Size

 MgSO4

Na Acetate

Na Citrate

Na Citrate Sesquihtdrate

NaCl

AOAC QuEChERS Extraction Bags for 15 g samples

00528-00000

100

6 g

 1.5 g

 

 

 

EN QuEChERS Extraction Bags for 10 g Samples

 00529-00000

 100

4 g

 

1 g

0.5 g

1 g

Original QuEChERS Extraction Bags 10 g Samples

 00530-00000

 100

 4 g

 

 

 

1 g

Step 2: Clean up

Choose right clean up tube, transfer the extracted sample in Step 1 to clean up tube, shake for 30 seconds, then centrifuge > 1500 rcf for 1 minute.

Welchrom® QuEChERS Clean-up Tubes Ordering Information

 

Part Numbers

Tubes

Pack Size

 MgSO4

PSA

C18E

GCB

AOAC Method fruits and vegetables

00531-20020

2 mL

100

150 mg

50 mg

 

 

00531-20021

 15 mL

50

1200 mg

400 mg

 

 

EN Method fruits and vegetables

00532-20020

2 mL

 100

150 mg

 25 mg

 

 

00532-20021

15 mL

  50

900 mg

150 mg

 

 

AOAC Method, Waxy or fatty fruits and vegetables

00533-20020

2 mL

100

150 mg

50 mg

50 mg

 

00533-20021

15 mL

50

1200 mg

400 mg

 400 mg

 

 EN Method Waxy or fatty fruits and vegetables

00534-20020

 2 mL

 100

 150 mg

25 mg

 25 mg

 

00534-20021

15 mL

50

 900 mg

150 mg

150 mg

 

AOAC Method Pigment containing fruits and vegetables

00535-20020

 2 mL

100

150 mg

 50 mg

 

50 mg

00535-20021

2 mL

  50

 1200 mg

 400 mg

 

400 mg

 EN Method Pigment content fruits and vegetables

00536-20020

 2 mL

100

150 mg

25 mg

 

2.5 mg

00536-20021

15 mL

50

900 mg

150 mg

 

15 mg

AOAC Method High pigment and fatty content fruits and vegetables

00537-20020

2 mL

100

150 mg

50 mg

50 mg

50 mg

00537-20021

15 mL

 50

1200 mg

 400 mg

400 mg

400 mg

EN Method High pigment content fruits and vegetables

00538-20020

2 mL

100

150 mg

25 mg

 

7.5 mg

00538-20021

15 mL

100

900 mg

150 mg

 

45 mg

 

What are these sorbents used for?

MgSO4
Magnesioum Sulfate

Removes excess H2O in the matrix

PSA
Primary / Secondary Amines

Removes the carbohydrate, sugars, fatty acid, organic acid and anthocyanine pigment in the matrix

C18E
Endcapped C1 Sorbents

Removes fat, sterols and other non-polar compounds in the matrix

GCB
Graphitised Carbon Black

Remove pigment, sterol in the matrix. Not for use with planar pesticides

 

 

Sorbent Name 

Part Number

Unit

Packing Size

Wellchrom  powder

PSA

 00508-11018

g

100

NH2 

00509-11018

g

100

C18E  

00501-11018

g

100

GCB

00517-20018 

g

100

Welchrom Quet Pack

PSA

00508-11004

200 mg/3 mL

50

00508-11006 

500 mg/6 mL

30

NH2

00509-11004 

200 mg/3 mL

50

00509-11006 

500 mg/6 mL

30

C18E

00501-11004 

200 mg/3 mL

50

00501-11006 

500 mg/6 mL

30

GCB

00517-20004 

200 mg/3 mL

50

00517-20006 

500 mg/6 mL

30

Welchrom Syringe pack

Carb/NH2

00527-20010

250 mg/250 mg, 3 mL

50

00527-20011

 500 mg/500 mg, 6 mL

30

 

Clean-up result from Spinage using different content of Welchrom® GraphiCarb

Wellchrom clean up

 

Application: the analysis of multi-residue pesticide in apple using Welchrom QuEChERS Kit

Homogenizing Sample

Welchrom sample prep

Extraction

  1. Transfer homogenized sample (10g or 15g) to 50ml tube
  2. Add 15ml ACN with 1% HOAC
  3. Add internal standard
  4. Shake for 1 min
  5. Add 00529-00000 extraction salt into tube
  6. Shake for 1 min
  7. Centrifuge > 4000rpm for 5 min
  8. Get the above liquid

Clean up

  1. Add 1 ml above liquid sample into 00531-20020 clean up tube, shake for 1 min
  2. Add 8 ml above liquid sample into 00532-20021 clean up tube, shake for 1 min
  3. Centrifuge > 13000 rpm for 2 min
  4. Get the above liquid for injection

GC Method

  • Autosampler: Agilent 7683 AS
  • Column: Welchrom WM-5MS GC Column,30 m*0.25 mm, 0.25um
  • Gas: He
  • Temp: 70 C (2min) ,25 C/min to 150 C (0min), 3C/min to 200C (0min), 8C/min to 280C
  • Volume: 1.0ul

Mass Method

  • Mode: SIM
  • Source: Quadrupole
  • Interface Temp: 230C, 150C and 280C
Wellchrom MS

Results

 

10 ng/g 

50 ng/g

200 ng/g

Target

Recovery

RSD(n=6)

Recovery

RSD(n=6)

Recovery

RSD(n=6)

Dichlorvos 

86.8

7.0

83.9

11.6

81.5

5.5

2-Phenyl phenol

113.4

6.3

96.3

6.5

100.5

 3.6

Diazinon   

98.6

2.3

87.3

2.8

90.4

4.9

Chlorothalonil

86.1

10.0

84.4

2.9

93.2

 7.6

Carbaryl

96.1

9.0

93.8

   8.3

99.1

8.2

Dichlofluanid 

 90.0

7.0

84.6

2.9

94.6

5.0

Dichloroacetophenone  

97.8

7.5

95.5

6.2

104.5

4.5

N-(trichloromethylthio) phthalimide  

-

-

74.4

9.3

94.6

10.3

y-Chlordane

9.6

4.7

88.8

4.6

 95.3

 3.4

Endosulfan 

 69.8

9.2

91.3

 5.4

96.2

5.4

Dieldrin

90.5

10.3

86.6

3.4

92.8

4.8

DDE 

 84.0

4.8

89.4

3.8

95.4

4.5

Ethion  

 90.9

1.8

103.5

2.8

113.4

 6.2

Endosulfansulfate 

79.8

 1.9

80.4

4.6

86.9

 5.5

Endrin Ketone Standard   

85.2

10.4

80.7

4.3

92.7

4.5

Permethrin

87.9

2.7

93.8

2.0

 94.0

 4.6

Coumaphos  

87.8

5.1

 88.2

 3.3

 92.3

6.5

 

 

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