Logochromatographyshopneu
Merck Lichro

Merck based Silica Hichrom packed columns

The product range comprises

 

Lichrosorb

  • Irregular porous silica
  • 5 and 10μm particle sizes
  • Hichrom high efficiency
  • Diol phase

LiChrosorb® is a traditional silica manufactured by Merck KGaA, and available as 60 and 100 pore size particles.LiChrosorb RP-select B was one of the first base deactivated silicas to be developed. All Hichrom columns are compatible with the Merck Hibar® RT range of columns, but use industry  standard compression fittings.

LiChrosorb
Phase

Functional Groups

Endcapped

Particle Size
(μm)

Pore Size ()

Surface Area
(m2/g)

Carbon Load
(%)

Silica

 

 

5, 10

60, 100

490, 300

 

RP-8

Octyl

 No

5, 10

100

300

 10

RP-18

 Octadecyl

No

5, 10

100

300

 17

CN

Cyano

No

5, 10

100

 300

7

NH2

Amino

 No

5, 10

100

300

4

Diol

Hydroxyl

No

5, 10

100

300

8

RP-select B

 Octyl

Yes

 5, 10

100

 300

 11

 

Lichrospher

  • Spherical porous silica
  • 5μm (Superspher® 4μm) particle size
  • Base deactivated LiChrospher RP-select B
  • Endcapping options for RP-8 and RP-18

Hichrom high efficiency  LiChrospher® is a traditional silica manufactured by Merck KGaA. The availability of both an endcapped and nonendcapped version significantly enhances the chromatographer’s selectivity options. LiChrospher RP-select B was Merck’s first generation base deactivated material. A range of 4μm materials have been marketed under the Superspher®1 tradename in Europe.

Lichrosphere phases

LiChrospher
Phase

Functional group

Endcapped

Particle Size2
(μm)

Pore Size ()

Surface Area
(m2/g)

Carbon Load
(%)

Si-60

 

 

5

  60

700

 

Si 100

 

 

5

100

400

 

100RP-8  

Octyl

No

5

100

350

12.5

100RP-8e 

Octyl

Yes

5

100

350

13.0

100RP-18  

Octadecyl

No

5

100

350

21.0

100RP-18e   

Octadecyl

Yes

5

100

350

21.6

100NH2   

Amino

No

5

100

350

4.6

100CN

Cyano

 No

5

100

350

 6.6

100Diol    

Hydroxyl

No

5

100

350

 8.0

60RP-select B 

Octyl

Yes

5

60

360

11.5

1 Superspher columns available on request. 2 LiChrospher 10μm particle size materials available

 

Column Sizes available

  1. Microbore (1.0mm i.d.) Columns
  2. Microbore (2.1mm i.d) Columns
  3. Medium Bore (3.2mm i.d. Columns)
  4. Analytical (4.0mm i.d.) Columns
  5. Analytical (4.6mm i.d.) Columns
  6. Semi-Preparative and Preparative (7.75 - 21.2mm i.d.) Columns

 

Merck Cartridge System

LiChrosorb®, LiChrospher® and Superspher® are available in Merck's LiChroCART® cartridge system used with manu-CART® endfittings. These columns can be used with or without an integral guard cartridge. The integral guard can be incorporated on reversing the split collets within the endfittings (Figure 1). Merck Hibar RT columns are also available on request.

Merck fig 1

 

Lichroprep

  • Irregular preparative silica
  • Four particle size ranges
  • Guaranteed reproducibility from analytical (LiChrospher) to preparative phases
  • Competitive price

Merck offer four different particle size ranges of LiChroprep®  silica, available as a base silica or C18 bonded phase. The two smaller particle size ranges, 5-20μm and 15-25μm, are the most suitable for HPLC. LiChroprep RP-select-B, amino-, cyano- and diol-bonded phases are available in larger particle size materials.

Selectivity and Reproducibility

Selectivity is one of the most important factors in chromatography. It determines both the quality and reproducibility of a chromatographic separation and, on a preparative scale, provides a decisive influence on the yield per unit time. Figure 1 shows the guaranteed selectivity parameters.

 

Lichroprep RP-18 Phase

Particle  Size Range (μm)

5-20

15-25

25-40

40-63

Mean Particle Size (μm)

 12 

 22

32

54

Minimum Theoretical Plates (plates/m)1

15,000

10,000

 5,500

 1500

Typical Column Back Pressure (psi)1

 300

150

90

 75

Surface Area (m2/g)

300

Pore Size ()

100

Carbon Load (%)

17-19

 

Purospher RP-18 and RP-18e

Purospher® RP-18 is a base deactivated material based on high purity porous spherical silica. It is well suited to the analyses of neutral, basic and weakly acidic compounds. A hydrophobic endcapped version (Purospher RP-18e) has been designed for the elution of both basic and strongly acidic compounds.

Aluspher® RP-select B

Aluspher RP-select B is based on porous spherical alumina with surface modification by polymer coating with polybutadiene. It is a reversed-phase material, stable over the pH range 2 - 12. This gives Aluspher RP-select B the advantage over silica phases of the possibility of chromatography with basic eluents. In this way, the ionization of basic compounds is depressed and peak tailing is avoided.

Purospher STAR Phases

Purospher STAR phases are based on high purity silica and designed for the analysis of basic, neutral and metal chelating samples. Purospher STAR RP-18e has higher pH stability (1.5 to 10.5) than Purospher RP-18 and RP-18e.

ChiraDex® Chiral Phases

In the ChiraDex range of phases, a β-cyclodextrin is bonded to a porous spherical silica. Within the cavities of this cyclic oligosaccharide, enantiomeric solutes can be made to form 'diastereomeric complexes'. The extent of this complexation can be used to separate enantiomeric molecules. ChiraDex shows broad enantioselectivity.

Phase Range

Phase

Description

Particle Size (μm)

 Pore Size ()

Surface Area (m2/g)

Purospher RP-18

C18 phase with polar (amino) endcapping

5

90

500

Purospher RP-18e

C18 phase with hydrophobic endcapping

5

120

350

Purospher STAR RP-18e 

C18 phase with hydrophobic endcapping

2, 3, 5

120

330

Purospher STAR RP-8e 

C8 phase with hydrophobic endcapping

 3, 5

120

330

Purospher STAR Si

Unbonded silica

 5

120

330

Purospher STAR NH2

Amino bonded silica

5

120

 330

Aluspher RP-select B

Polybutadiene coated spherical alumina

5

 100

170

ChiraDex

β-Cyclodextrin bonded porous spherical silica

5

100

350

 

Chromolith™

  • Monolithic silica column with bimodal pore structure
  • Reduced back pressure compared with particulate silica
  • Fast analysis, high throughput

Chromolith™ HPLC columns consist of monolithic rods made of highly porous metal-free silica. The production of this material is based on 'sol-gel' technology which results in rods of polymerized silica that are mechanically stable and chemically resistant. After derivatisation and endcapping, where appropriate, the silica rod is encased in a PEEK plastic cover. The Chromolith material consists of a bimodal structure. A dense network of macropores, each 2μm in diameter, serves as through pores, creating a high total porosity. Within the skeletal structure of the rod is a further network of mesopores, each 13nm in diameter, which creates the large internal surface area over which chemical adsorption can take place.

Merck Fig 2
Fig 2

Chromolith Phases

Particle Size

 Monolithic

Macropore Size

2μm

Mesopore Size

13nm (130)

Total Porosity

 >80%

Surface Area

300m2/g

Carbon Load

18% (RP-18e)
11% (RP-8e)

 

Features of Chromolith Columns

Selectivity

Chromolith columns show comparable selectivity to conventional reversed-phase columns, so existing methods can be easily transferred with only minimal method development. However, retention times are shorter on the Chromolith column.

High flow rates

Due to the very high total porosity of the Chromolith column, very high flow rates (up to 10ml/min) can be applied with very low back pressures. Since efficiency does not decrease with increased linear velocity as much as with traditional particulate columns, flow programming is feasible (see Figure 3).

Merck Fig 3

Added column performance

Several Chromolith columns can be linked in series producing a column with a theoretical plate count significantly higher than particulate columns

 

 

© by LCC 1995 -2016 All rights reserved
 Chromatographyshop is a division of LCC Engineering & Trading GmbH, CH-4622 Egerkingen, Switzerland.
Collaboration partners in various countries
Our Company Profile and Summary Product List 2015
Unser Firmenprofil und Produktzusammenfassung 2015
      Our Terms and Conditions

Sample Preparation Tools

[Sample  Preparation]
[MatrixEx]
[QuEChERS]
[SPE]
[Syringe Filters]
[Inert RSA  Glass Vials]
[Flash Chromatography]

General Issues

[Chromatogr. Shop Home]
[Newsletter Archive]
[Our Services & Company]
[Terms & Conditions]
[Mobile Phone]

 Bonuses for you

[Special Deals for you]

Seminars & Workshops

[Knowledge Education]
[Seminars & Workshops]

Analytical Scale Chromatography

[Analytical Columns]
[ACE & ACE  Excel Columns]
[ACE Ultra Core]
[Microsolv Cogent Column]
[Capcell Shiseido]
[Daicel / Chiral Technologies]
[GL Science  Inertsil]
[Hichrom Columns]
[Imtakt Column]
[Kromasil Akzo Nobel]
[Mitsubishi MCI-Gel]
[Merck Lichro...]
[Macherey &  Nagel]
[Princeton  Chromatography]
[PolyLC  Columns]
[SieLC Primesep]
[Capcell Shiseido]
[Ultron by Shinwa]
[Welch  Materials]
[Zirchrom]
[Agilent Zorbax]

Knowledge Tools for Chromatographers

[Knowledge Education]
[Definition of  Terms Used]
[Separation Mechanism]
[Solvent  Selection]
[Buffers for  LC.]
[Column &  Material Care]
[Prep & Process Columns]
[Analytical  Columns]
[HPLC- to-UHPLC]

Resin based Systems (Analytical to Process scale)

[Bioseparation Biopurification]

Special Tools to improve data quality, reproducibility and productivity

[Inert RSA  Glass Vials]

Method & Material  Choices  1

[Knowledge Education]
[Sample  Preparation]
[Reverse_Phase_LC]
[Adsorption-LC RPC & SPE]
[AQ Columns]
[Phenyl Bond Columns]
[FPF_Columns]
[HILIC & Aq. Norm. Phase]
[Electrostat. Repul. HILIC]
[Normal Phase Chromatography]

Process Scale   Chromatography Equipment

[Process  Chromatogr.]
[Process Chromat.Systems]
[Flash &  MPLC Pumps]
[HPLC  Pumps]
[Special  Pumps]
[Fraction Collectors]
[Stainless Steel Columns]
[Glass  Columns]
[Glass  Cartridges]
[Plastic Cartridges]
[Manual  Valves]
[Motor  Valves]
[Efficiency  Tools]

Method & Material Choices 

[Knowledge Education]
[Multi  Mode LC]
[Chiral  Chromatography]
[Ion Exchange  & Ion LC]
[Hydrophobic  Interaction LC.]
[Affinity  LC]
[Size Exclusion Chromatogr.]
[SuperCritical Fluid LC]
[Proteomics]
[Metabolomics]
[Process  Scale LC]
[Kamasutra Inspired Tech]