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Macherey & Nagel

Broad range of phases for small and large molecules

The product range comprises

  1. NUCLEOSIL
  2. NUCLEOSIL® Base Deactivated Phases
  3. NUCLEOSIL® C18 Nautilus
  4. NUCLEODUR® Bulk Material
  5. NUCLEODUR® Columns
  6. NUCLEODUR® Gravity
  7. NUCLEODUR® C18 ec and C8 e
  8. NUCLEODUR® C18 Isis
  9. NUCLEODUR® C18 Pyramid
  10. NUCLEODUR® Sphinx RP
  11. NUCLEODUR HILIC
  12. NUCLEODUR® 1.8μm Phases
  13. NUCLEOGEN® Columns for Bioanalysis
  14. NUCLEOGEL® Columns for Food Analysis
  15. NUCLEODEX® Chiral Phases
  16. RESOLVOSIL® Chiral Phases
  17. NUCLEOSIL® Chiral Phases
  18. NUCLEOCEL Chiral Phases

 

NUCLEOSIL

  • Spherical porous silica
  • Wide range of bonded phases
  • Excellent mechanical stability

NUCLEOSIL®, a classical silica manufactured by Macherey-Nagel GmbH & Co. KG is available in a wide range of pore sizes. NUCLEOSIL 100 and 120 have similar relative retention characteristics. NUCLEOSIL 100 has the greater capacity whilst the 120 material exhibits the lower column back pressure. The wide pore NUCLEOSIL 300 silica is used as the base material for the reversed-phase bioanalytical columns.

NUCLEOSIL 100-5C18AB was the first of the new generation NUCLEOSIL materials which include the HD high density phases NUCLEOSIL Protect 1 and the Nautilus, for use with up to 100% aqueous eluents

NUCLEOSIL Silicas

Material 

Pore Diameter ()

Surface Area (m2/g)

Particle Size (μm)

NUCLEOSIL 100

100

 350

3, 5, 7, 10

NUCLEOSIL 120

120

200

3, 5, 7, 10

NUCLEOSIL 300

300

100

5, 7, 10

NUCLEOSIL Phases

NUCLEOSIL Phase

Functional Group

Pore Size ()

Particle Size (μm)

Carbon Load (%)

Endcapped

C2 

Dimethyl

100

7

3.5

 No

C4   

Butyl

300

5

2

Yes

C8

Octyl

100
120
300

5, 7, 10
3, 5, 7, 10
5

8.5
6.5

No
No
No

C18

Octadecyl

100
120  
300

3, 5, 7, 10
3, 5, 7, 10
5

15
11
6.5

Yes
Yes
Yes

C18AB

Octadecyl
Polymeric
modification

 100

 5

24

 Yes

C6H5

Phenyl

100 
120 

5, 7
5, 7

8
6.5 

No
No

CN

Cyano

100
120

5,10
7

5

No

NH2

Amino

100
120

5
7

3

No

NO2

Nitro

100 

5

 -

No

OH  

Diol

100

7

 5

No

SA

Sulphonic Acid

100

5, 10

 6.5

No

SB

Quaternary
Ammonium

100

5, 10

10

No

POLYGOSIL, wider pore NUCLEOSIL (500, 1000 and 4000) and columns for special applications eg PAH can be supplied on request

NUCLEOSIL® 100 Hichrom Manufactured Columns

  • Microbore (1.0 - 2.1mm i.d.) Columns
  • Medium Bore (3.2mm i.d.) Columns
  • Analytical (4.0mm i.d.) Columns
  • Analytical (4.6mm i.d.) Columns
  • Semi-Preparative and Preparative (7.75 - 21.2mm i.d.) Columns

NUCLEOSIL® 120 - Hichrom Manufactured Columns

  • Microbore (1.0 - 2.1mm i.d.) Columns
  • Medium Bore (3.2mm i.d.) Columns
  • Analytical (4.0mm i.d.) Columns
  • Analytical (4.6mm i.d.) Columns
  • Semi-Preparative and Preparative (7.75 - 21.2mm i.d.) Columns

NUCLEOSIL® 300 - Hichrom Manufactured Columns

  • Microbore (1.0 - 2.1mm i.d.) Columns
  • Medium Bore (3.2mm i.d.) Columns
  • Analytical (4.0mm i.d.) Columns
  • Analytical (4.6mm i.d.) Columns
  • Semi-Preparative and Preparative (7.75 - 21 mm id) Columns

 

NUCLEOSIL® Base Deactivated Phases

NUCLEOSIL® 100-5 C18 AB. NUCLEOSIL® 100-5 C8/C18 HD and NUCLEOSIL® 100-5 Protect 1 represent three successive generations of base deactivated reversedphase HPLC columns, manufactured from purer silica. NUCLEOSIL® 100-5 C18 HD features a high-density mono-layer coverage. Due to the shorter chain and an incorporated group, NUCLEOSIL®100-5 Protect 1 exhibits a distinctive polar selectivity. The HD material is also offered in an octyl-bonded chemistry. Figure 1 compares the selectivity differences of four NUCLEOSIL® base deactivated phases. For the Protect 1 phase, elution of naphthalene and ethylbenzene is reversed.

M&N Fig 1

 

NUCLEOSIL Phase

C8 HD

C18 HD

 Protect 1

Functional Group

Octyl

Octadecyl, high density
surface coverage

High polarity, base deactivated

Pore Size ()

100

100

100

Particle Size (μm)

3, 5

3, 5

3, 5

Carbon Load (%) 

13

20

11

Endcapped

Yes

Yes

 Yes

 

NUCLEOSIL® C18 Nautilus

NUCLEOSIL® C18 Nautilus shows outstanding stability in highly aqueous media (>95%) due to a covalently bonded C18 silane with an embedded polar functional group. As well as the hydrophobic interactions between analyte and stationary phase shown by standard C18 phases, NUCLEOSIL C18 Nautilus also shows polar interaction via hydrogen bonding and dipole-dipole forces. This can influence retention time and improve selectivity, particularly for the separation of polar compounds. Figure 2 shows the stability of retention times with 100% aqueous eluents compared with the phase collapse shown by conventional RP phases.

M&N Fig 2

NUCLEOSIL® C18 Nautilus Phase

Pore Size ()

 Particle Size (μm)

Carbon Load (%)

 Endcapped

100

3, 5

16

yes

 

NUCLEODUR® Bulk Material

  • Ultra low metal content
  • Range of particle sizes
  • High loadability

NUCLEODUR® is an ideal material for scaling-up methods due to the availability of a wide range of particle sizes.The material provides a high mechanical bed stability and the narrow particle size distributions provide lower pressure drop due to the absence of fines. The material is reusable even after several cycles of repeated packing.

Particle Type

Spherical

Particle Size (μm)

10, 12, 16, 20, 30, 50

Pore Diameter ()

 110

Endcapped

Yes

Chemistries Silica,

C18

pH Range

1 - 9

 

NUCLEODUR® Columns

  • Ultrapure silica
  • Excellent mechanical and chemical stability
  • Low level metal ions
  • Enhanced pH stability

NUCLEODUR® is Macherey-Nagel's newest range of phases, based on ultrapure spherical silica. The special surface bonding technology and the low concentration of metal ions lead to high efficiency symmetrical peaks. NUCLEODUR® Phases

NUCLEODUR
Phase

Functional Group

Endcapped

Particle Size
(μm)

 Pore Size ()

Surface Area
(m2/g)

Carbon Load

C18 Gravity

Octadecyl

Yes

1.8, 3, 5

110

340

 18

C18 ec  

Octadecyl

Yes

3, 5

110

340

17.5

C8 Gravity  

Octyl

Yes

1.8, 5

110

340

11

C8 ec   

Octyl

Yes

3, 5

110

340

10.5

C18 Pyramid   

Octadecyl

Yes

1.8, 3, 5

110

340

14

C18 Isis  

Octadecyl

Yes

1.8, 3, 5

110

340

20

Sphinx RP 

Octadecyl/phenyl

Yes

1.8, 3, 5

110

340

15

CN and CN-RP

Cyano

Yes

3, 5

110

340

 7

NH2 and NH2-RP  

Amino

No

5

110

340

2.5

HILIC Zwitterionic 

ammonium sulphonic acid

-

1.8, 3, 5

110

340

7

C18 PAH

Octadecyl

Yes

3

110

340

proprietary

 

NUCLEODUR® Gravity

NUCLEODUR® C18 Gravity and C8 Gravity are based on the ultrapure NUCLEODUR silica. The high density bonding of these phases leads to an extended operational pH range (1-11). This can be helpful for improving selectivity in method development and for more challenging applications.

NUCLEODUR® C18 ec and C8 e

The NUCLEODUR C18 ec and C8 ec phases are recommended for routine analyses and for scaling up to preparative HPLC (see above for bulk materials).

NUCLEODUR® C18 Isis

NUCLEODUR C18 Isis is a C18 bonded phase with specially cross-linked surface modification. This polymeric bonding leads to high steric selectivity and is therefore suited for separation of positional and structural isomers and planar/non-planar molecules. The phase has an enhanced pH stability of 1 to 10. Figure 3 demonstrates the increased steric selectivity of NUCLEODUR C18 Isis for o-terphenyl (non-planar) and triphenylene (planar) compared with a monomerically bonded C18 phase.

M&NFig3

 

NUCLEODUR® C18 Pyramid

NUCLEODUR® C18 Pyramid is a C18 bonded phase with hydrophilic endcapping, designed especially for use with eluents containing up to 100% water. The phase also shows improved retention characteristics for very polar molecules, such as short chain organic acids (see Figure 4).

M&NFig4

 

NUCLEODUR® Sphinx RP

  • Bifunctional C18/Phenyl phase
  • High density bonding
  • Low LC-MS bleed

NUCLEODUR® Sphinx RP is a bifunctional phase consisting of a well-balanced ratio of octadecyl and phenyl groups covalently bonded to the NUCLEODUR silica. The combination of classical hydrophobic with π-π interactions expands the scope of selectivity in comparison with conventional reversed-phase materials. NUCLEODUR Sphinx RP is particularly suited for the separation of molecules containing aromatic and multiple bonds. It may also outperform standard C18 phases for the separation of polar compounds. Figure 5 shows a
comparison of the separation of flavonoids on three different NUCLEODUR phases.

M&NFig5

 

NUCLEODUR HILIC

NUCLEODUR HILIC is a zwitterionic modified stationary phase. The betaine character of the ammonium – charged, but highly polar surface. This column is ideal for analysing polar analytes such as acids and bases, natural compounds, nucleosides, amino acids, water soluble vitamins etc. Due to its high loadability, NUCLEODUR HILIC is suitable for preparative applications.

 

NUCLEODUR® 1.8μm Phases

  • Ultra fast HPLC
  • Shorter columns with high separation efficiency
  • Significant improvement in resolution
  • Increased detection sensitivity

Increase in separation efficiency All of the NUCLEODUR® premium phases are now available in 1.8μm particle sizes. Columns packed with these microspherical particles show improvements in terms of plate numbers, column efficiencies and resolution, compared with their 3μm counterparts

An increase in plate number of approximately 67% in transferring from 3μm to 1.8μm particles offers the possibility of using shorter columns with equal plate numbers, resulting in a decrease in analysis time. Typically, for a 50 x 4.6mm NUCLEODUR C18 Gravity column:

  • 3μm: N ≥ 100,000 plates/m
  • 1.8μm: N ≥ 166,667 plates/m

Improvement in resolution

The use of 1.8 μm instead of 3 μm particles can lead to an increase in resolution of approximately 30%. This is illustrated in Figure 6 for the separation of naphthalene and ethylbenzene

M&NFig6

 

Column back pressure

The smaller particle size results in an increased back pressure. However, the high sphericity of the NUCLEODUR particles and their very narrow particle size distribution means that back pressure is kept at a moderate level. In addition, the particles are fractionated in order to limit the increase in back pressure. Nevertheless, the use of columns packed with sub 2μm particles generally makes special demands on the HPLC equipment. Pumps should be designed for pressures of 250 - 1000 bar and the entire system should feature the lowest possible dead volume, for optimum column performance.

Higher flow rates and shorter run times

The optimal flow rate for 1.8 μm particles is higher than for 3 and 5 μm particles. Figure 7 shows the considerable decrease in analysis time on converting from a 5 μm to a 1.8 μm column and increasing the flow rate.

M&NFig7

 

Bioanalytical and Food Analysis Phases

Macherey-Nagel manufactures a range of ion-exchange (NUCLEOGEN®), reversed-phase and gel filtration (NUCLEOGEL® GFC) columns for biochemical analysis, and ligand exchange (NUCLEOGEL® Sugar) columns for food analysis.

 

NUCLEOGEN® Columns for Bioanalysis

  • Silica based DEAE anion exchangers
  • Available with pore sizes 60, 500 and 4000
  • Suitable for analysis of high MW bioanalytical molecules eg. deoxyoligonucleotides, high MW RNAs and plasmid DNAs

NUCLEOGEN
Phase

Pore Size ()

Particle Size
(μm)

Column Dimensions (mm)

Catalogue No.

60-7 DEAE

 60

 7

125 x 4.0

736596.40

60-7 DEAE

 60

7

125 x 10.0

736597.100

500-7 DEAE 

500

7

125 x 6.0

736598

500-7 DEAE  

500

7

125 x 10.0

736599.100

4000-7 DEAE

4000

7

 125 x 6.0

736601

4000-7-DEAE  

4000

7

125 x 10.0

736602.100

Guard column(2/pk)

 -

 -

30 x 4.0

736400.40

1 Suitable for all NUCLEOGEN columns, Particle size 25-40μm., Use with holder 721823

 

NUCLEOGEL® Columns for Food Analysis

  • Sulphonated polystyrene-divinylbenzene resins
  • Long column lifetimes
  • Excellent pH stability
  • Use with up to 30% CH3CN

The NUCLEOGEL Sugar 810 series is based on spherical sulphonated polystyrene-divinylbenzene resin in different ionic forms. Separations of carbohydrates, organic acids and other polar small molecules are achieved by a combination of ion exclusion, ion exchange, size exclusion, ligand exchange and partition mechanisms.

NUCLEOGEL Phase

Ionic Forms

 Recommended Applications

Sugar 810H

SO3H

Sugars, sugar alcohols, organic acids

Sugar 810Ca

SO3Ca

 Mono-, di- and oligosaccharides

 

M&NFig8

 

MACHEREY-NAGEL CHIRAL

  • Cyclodextrin cavity
  • BSA protein-bound
  • Ligand exchange
  • Polysaccharide

Macherey-Nagel manufactures a range of chiral columns. These include NUCLEODEX (cyclodextrin cavity), RESOLVOSIL (protein-bound), Chiral-1 (ligand exchange) and the new NUCLEOCEL ALPHA and DELTA

 

NUCLEODEX® Chiral Phases

NUCLEODEX® phases are manufactured by chemically bonding one of three cyclodextrins to NUCLEOSIL 100 silica. Cyclodextrins are cyclic oligosaccharides. The size of the chiral ring and interaction of racemic mixtures with the chiral sugar units control the enantiomeric separation process. In the permethylated cyclodextrins all hydroxy groups are replaced by methoxy groups. Alternative selectivities are obtained. NUCLEODEX® screening kits for rapid and economical method development are available. Each kit contains a 30 x 4mm cartridge of each of the four NUCLEODEX materials, -OH, -PM, -PM and -PM, together with a holder.

 

RESOLVOSIL® Chiral Phases

RESOLVOSIL® BSA-7 was one of the first commercially available protein-bound columns to demonstrate optical resolution.

 

NUCLEOSIL® Chiral Phases

NUCLEOSIL® chiral-1 is a unique material offering separation of racemic molecules by ligand exchange HPLC. An L-hydroxyproline Cu2+ complex is chemically bonded to NUCLEOSIL 120 silica. Molecules containing two correctly spaced polar functional groups, such as -amino acids can be optically resolved by this column through the formation of diastereomeric complexes of differing stability.

 

NUCLEOCEL Chiral Phases

  • Polysaccharide-coated phases
  • Separate wide range of chiral compounds
  • Columns for NP and RP applications
  • High loadability for up-scaling
  • Pressure stable to 2000psi

Macherey-Nagel have introduced the NUCLEOCEL series of polysaccharide chiral stationary phases. The chiral selector of NUCLEOCEL DELTA is cellulose-tris-3,5-(dimethylphenyl) carbamate and together with the supramolecular helical polysaccharide surface structure is mainly responsible for the chiral recognition mechanism. For NUCLEOCEL ALPHA the amylose counterpart of tris-3,5-(dimethylphenyl) carbamate performs this task. The NUCLEOCEL product line includes columns for normal-phase and reversed-phase applications. The NUCLEOCEL DELTA and ALPHA phases are intended for analyses using normal-phase eluents, typically heptane - IPA. The NUCLEOCEL DELTA-RP and ALPHA-RP phases are designed for use under reversed-phase conditions either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate. Figures 9 and 10 show examples of separations on these columns.

M&NFig9

 

In addition to the 10μm phases (DELTA and DELTA-RP), higher resolution 5μm phases are available for these and for the ALPHA and ALPHA-RP phases.NUCLEOCEL chiral stationary phases offer high loading capacities and thus are suitable for scale-up methods. The stable silica support protects the coated polysaccharide and tolerates high flow rates with a maximum operating pressure up to 2000 psi.

M&NFig10

 

Application range:

  • pharmaceutical active compounds
  • chiral pollutants, eg. herbicides, PCBs
  • chiral compounds in foodstuffs, dyes, preservatives
  • chiral catalysts and bio-organic compounds

 

Material

Description

Particle Size (μm)

Pore Size ()

 Dimensions (mm)

Catalogue No.

NUCLEODEX beta-OH

Cyclodextrin cavity NUCLEODEX beta-OH cyclodextrin

5

100

200 x 4.0

720124.40

NUCLEODEX beta-OH guard cartridge1

Cyclodextrin cavity NUCLEODEX beta-OH cyclodextrin

5

100

8 x 4.0 

721460.40

NUCLEODEX alpha-PM cyclodextrin

Permethylated alpha -cyclodextrin cavity

5

100

200 x 4.0

720127.40

NUCLEODEX alpha-PM guard cartridge1

Permethylated alpha -cyclodextrin cavity

5

100

8 x 4.0

721464.40

NUCLEODEX beta -PM cyclodextrin

Permethylated beta-cyclodextrin cavity

5

100

200 x 4.0

720125.40

NUCLEODEX beta -PM guard cartridge1 

 Permethylated beta -cyclodextrin cavity

5

100

8 x 4.0

721462.40

NUCLEODEX -PM cyclodextrin

 Permethylated gamma -cyclodextrin cavity

5

100

200 x 4.0

720752.40

NUCLEODEX -PM guard cartridge1

 Permethylated gamma-cyclodextrin cavity

5

100

8 x 4.0

721466.40

NUCLEODEX screening kit (4 columns)

 

5

100

30 x 4.0

721920

RESOLVOSIL BSA-7 300 pore, protein-based

RESOLVOSIL BSA-7 Bovine serum albumin (BSA) protein

7

300

150 x 4.0

720046.40

RESOLVOSIL BSA guard cartridge1

RESOLVOSIL BSA-7 Bovine serum albumin (BSA) protein

7

300

8 x 4.0

721702.40

NUCLEOSIL Chiral-1 L-hydroxyprolin e-Cu2+ complex

NUCLEOSIL Chiral-1 L-Hydroxyproline-Cu2+ complex ligand exchange

 5

120

250 x 4.0

 720081.40

NUCLEOSIL Chiral guard cartridge1

 

5

120

8 x 4.0

721455.40

NUCLEOCEL ALPHA

 Amylose-tris-3,5-(dimethylphenyl)-carbamate

5

 

150 x 4.6
250 x 4.6
Guard Cart.1
(4mm i.d.)

720644.46
720645.46

721000.40

NUCLEOCEL ALPHA-RP

Amylose-tris-3,5-(dimethylphenyl)-carbamate

5

 

Please enquire

 

NUCLEOCEL DELTA S

Cellulose-tris-3,5-(dimethylphenyl)-carbamate

5

 

Please enquire

 

NUCLEOCEL DELTA

Cellulose-tris-3,5-(dimethylphenyl)-carbamate

10

 

Please enquire

 

NUCLEOCEL DELTA-RP S

Cellulose-tris-3,5-(dimethylphenyl)-carbamate

5

 

Please enquire

 

NUCLEOCEL
DELTA-RP

Cellulose-tris-3,5-(dimethylphenyl)-carbamate

10

 

Please enquire

 

Use with guard column adaptor EC 721359

 

 

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