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Kromasil from Akzo Nobel

 Kromasil manufactures silica particles since many years and has pioneered many new products. The brand name is well establish in analytical and process applications

Kromasil is a highly recommended, robust, pure silica manufactured by EKA Chemicals AB of Sweden a subsidiary of EKA Noble. Kromasil is designed for analytical and preparative HPLC applications and is available in bility, pore structure and reproducible surface.

Development and production of Kromasil packaging are ISO 9001 Certified

LCC is sole distributor of Kromasil for Switzerland since many years.

Kromasil product range comprises of

New

 

Kromasil 60, 100 and 300 A

  • Spherical porous  silica
  • Analytical to preparative particle sizes
  • Robust and reproducible
  • Large surface area
  • Competitively priced
  • Excellent Batch to batch reproducibility
  • Excellent Chemical Stability
  • Analytical to prep scale applications

 

Kromasil Phase

Functional Group

Endcapped

Particle size um

Pore size A

Surface Area m2/g

Carbon Load %

Silica

 

 

5,7,10,13, 16

60

540

 

Silica

 

 

3.5,5,7,10,13, 16

100

320

 

C1

Methyl

Yes

5

100

320

4.7

C4

Butyl

Yes

1.8, 2.5, 3.5,5,7,10,13, 16

100

320

8.0

C8

Octyl

Yes

1.8, 2.5, 3.5,5,7,10,13, 16

100

320

12

C18

Octadecyl

Yes

1.8, 2.5, 3.5,5,7,10,13, 16

100

320

20

NH2

Amino

no

3.5,5,7,10,13, 16

100

320

1.7

CN

Cyano

No

5,10,16

60

540

12

Diol

Dihydroxy

No

5,10,16

60

540

10

Phenyl

Phenyl

Yes

5,10,16

100

320

14

Silica

 

 

5,10,16

300

110

 

C4

Butyl

Yes

5,10,16

300

110

2.9

C8

Octyl

Yes

5,10,16

300

110

4.7

C18

Octadecyl

Yes

5,10,16

300

110

8.7

NEW

Kromasil SFC - Designed for green technology

Designed for fast separations and based on 2.5 Ám particle size technology, the new Kromasil SFC columns are  tailor-made for research, discovery and routine analysis. The new  columns are delivered in cyano , diol, silica, and 2-ethylpyridine  chemistries for the laboratory scientist to separate and analyse a wide  range of substances, from non-polar to strongly polar compounds.

kromasil_sfc

Kromasil Eternity XT

Desigend for Extended  Life

kromasil_eternityxt_columns

kromasil_eternityx

Kromasil HILIC

Optimal selectivity of polar compounds with an MS compatible phase

kromasil_hilic

 

Kromasil 2.5 UHPLC/HPLC Columns

The new Kromasil 2.5 um based on 100 A product line and available with C4, C8 and C18 derivatisation. The particles are packed in UHPLC column of the following dimensions 2.1 x 50 mm, 2.1 x 100 mm, 4.6 x 50 mm and 4.6 x 100 mm 

Brochure: Kromasil_2 5_Ám_ 28022013

Batch to Batch Reproducibility

Kromasil material is manufactured using the well-known sol-gel technique which gives the resultant silica optimized
chemical and mechanical stability, pore structure and reproducible surface area. Development and production of the Kromasil packings are ISO 9001:2000 certified. Extensive control and testing is performed throughout the whole production process, ensuring the highest possible reproducibility. Proof of this can be seen in Figures 1 and 2, showing the reproducibility of batches of Kromasil. Kromasil packings for process scale HPLC are manufactured
in batches of over 100kg, within a modern plant whose capacity is several tonnes per year.

Kromasil Fig 1 and 2

Chemical Stability

At low pH the major mechanism of column degradation is acid hydrolysis of the bonded phase. The nature of the surface silanols and the coating density of the silane are key parameters. In Figure 3 the advantageous chemical stability of Kromasil® C8 is compared with other silicas.

Kromasil Fig 3

At pH 7 or higher, the major mechanism of degradation is dissolution of silica in aqueous mobile phases. The mode of manufacture of the silica, its surface treatment and the coating density of the silane, all affect higher pH stability. Kromasil’s stability in alkaline conditions was tested at ambient temperature by eluting a Kromasil 10-C8 column (200 x 4.6 mm) with CH3OH-NH4Ac/NH3, pH 9.25 (70:30) at a flow rate of 4.0 ml/min. for a prolonged period. The total number of column volumes was 10,000. Minimal change in column performance is seen after the test period.

Scalability

The following example (Figure 4) demonstrates the reproducible chromatography obtained when scaling up from a 4.6mm i.d. column to a 50.8mm (2") i.d. column.

Kromasil Fig 4
Kromasil 2-5 scale up

 

Kromasil 1.8 UHPLC Columns

Kromasil 1.8 μm is based on the 100 A  product line, and is available with C4, C8 and C18 derivatizations, in 2.1 mm I.D. UHPLC columns.

Brochures:

kromasil_1.8_beyond_expectations

Kromasil_1 8_beyond_expectations_Summary

 

Kromasil 300 A Column for Biochromatography

  • 300 A spherical silica
  • For Biomolecules that are larger than 8 - 10 kDa
  • Protein Separation from analytical to process scale
  • Narrow pore size distribution  ensures good mass transfer for molecules in this range resulting narrow peaks and no size exclusion effects
  • High mechanical and chemical stability
  • Available in
  • Micro-bore (1.0 - 2.1 mm Column
  • Analytical
  • Semi-prep (7.75 - 10 mm i.d.) Column
  • Prep (21.2 to 30 mm id) Column

Kromasil® 300┼ phases are manufactured to exhibit a surface chemistry similar to the well established Kromasil 100┼ phases. They are designed for the analysis of proteins and biomolecules larger than 8 - 10 kD. A narrow pore size distribution ensures good mass transfer for molecules in this range, resulting in narrow peaks and no size exclusion effects. The high mechanical stability of the material is especially important for large diameter columns


Preparative and Process Scale Kromasil

  • Scalable, Manufactured in large batches prior fractionation into narrow particle sizes
  • Mechanical strength (Maybe packed in columns with large pressures)
  • Superior load-ability (high pore volumes and surface area resulting in high load-ability without weakening their mechanical strength
  • Technical service

Scalability

Kromasil phases are manufactured in large batches prior to fractionation into narrow particle size ranges with the same characteristics. Hence scale-up from analytical to process size operations is easily achieved.

Mechanical Strength

In large diameter dynamic axial compression HPLC systems, columns have to be packed at high piston pressures to achieve optimum performance. Often the column is repacked. Silica particle mechanical strength is crucial for optimum phase lifetime and for a problem free process. Kromasil particles retain a high mechanical strength. Kromasil® is pure, chemically stable and reproducible from one silica batch to another. Its mechanical strength, scalability and superior loadability also give it advantageous preparative properties. Kromasil is available in bulk or in large preparative columns.

Loadability

Kromasil silica particles are characterised by high pore volumes and surface areas resulting in high loadability without weakening their mechanical strength.

Technical Service

Using KromaGuide software Eka Chemicals offer a powerful  tool for optimising running conditions. From a few analytical experiments and cost factor information for solvents, equipment etc. they can predict optimal running conditions such as sample loading, column dimensions, flow rate and particle size


 

Kromasil Eternity Hybride materials

Benefits

  • Modified silica
  • Small hybride particles (2.5 um) for uHPLC or HPLC conditions
  • Long lasting columns
  • Operation condition pH 1 - 12
  • Two surface Chemistries C18 and Phenyl
  • Easier method development

 

Kromasil Eternity surface
Very pH stable surface chemistry

Change selectivity by changin pH

Kromasil Eternity pH variance

 

 

Kromasil_Eternity_Brochure

Kromasil_Eternity_Phenyl_Hexyl_Brochure

Kromasil_Eternity_PhenylHexyl_Brochure (Small)

Kromasil_eternity_poster_new_analytical_product_Pittcon_2009

Kromasil_Ethernity_hplc_2010_utility_of_a_chemically_stable_aromatic_stationary_phase

 

 

Kromasil AmyCoat & CelluCoat Chiral Phases

Benefits

  • A new material and new chiral chemistry
  • 3, 5 and 10μm particle sizes
  • Increased pressure limits
  • Negligible memory effects
  • Fully scaleable
  • Suitable for HPLC and SFC applications
  • Polysaccaride based chiral HPLC
  • Analytical and prep application
  • No pressure limits, Mechanically strong
  • High resolution
  • Unique coating method
  • Wide range of compatible mobile phases

 

Kromasil_Amycoat_Phase_Picture

Kromasil® AmyCoat™ and CelluCoat™ are new generation polysaccharide-based phases. High purity deactivated wide pore silica is coated with tris(3,5- dimethylphenyl)carbamoyl functionalised amylose or cellulose selector (see below). This is performed  using a unique deposition procedure.

 

Kromasil_Chemical_Structure_Amycoat
Kromasil_Chemical_Structure_Cellucoat

 

Features

Excellent resolution and selectivity

Both Kromasil AmyCoat and Kromasil CelluCoat show excellent enantioselectivity for many different racemates. The availability of 3μm particles enables higher efficiency and resolution for analytical separations. This improved resolution allows a higher flow rate for faster analyses. The example below shows baseline separation of the enantiomers of Troger's base in less than 1 minute.

Kromasil fast analysis
Fast analytical chromatography on Kromasil AmyCoat

 

Fully back-integrated

Eka Chemicals manufacture the super wide pore silica for Kromasil AmyCoat and Kromasil CelluCoat and perform all subsequent steps leading to the final product. This means that the products have full traceability and Eka can control the complete process.

Increased pressure limits

Kromasil AmyCoat and CelluCoat phases can withstand flow rates equivalent to pressures up to 400 bar - the approximate upper limit for most HPLC systems. This enables faster analyses to be performed. Stable performance Switching between compatible normal-phase and polar organic eluents will not lead to any degradation in performance. This means that columns do not need to be dedicated to a particular solvent

Stable performance

Switching between compatible normal-phase and polar organic eluents will not lead to any degradation in performance. This means that columns do not need to be dedicated to a particular solvent.

RP Phases

The newer Kromasil CelluCoat RP and AmyCoat RP phases are based on the same silica matrix and chiral selector as the corresponding Kromasil CelluCoat and AmyCoat phases. These 3μm particle size materials are designed for fast, efficient and high resolution analyses under reversed-phase conditions.

Short equilibration times

The ability to use higher flow rates leads to short  equilibration times. In general, long equilibration times are most pronounced when switching eluents containing basic additives to acidic additives and vice versa. The Figure below illustrates the short equilibration times required for Kromasil CelluCoat when switching additives.

Kromasil ami fast equilibration

 

Easy to scale up

With particle sizes from 3 to 10μm giving identical selectivity, Kromasil AmyCoat and Kromasil CelluCoat make it easy to scale up whilst retaining excellent performance. Method development can be performed using 3μm analytical scale columns and translating the generated data to a larger column packed with 10μm particles. However, if you plan to scale up the process, it is recommended to use a 10μm analytical column from the start.analysis of metoprolol on Kromasil CelluCoat. High yields and enantiomeric purity were achieved on a 250 x 4.6mm column. If this separation were to be scaled up to a 250 x 50.8mm i.d. column, the equivalent loading of metopropol would be 2.4g.

Kromasil celucoat scale up

 

Kromasil_AmyCoat Brochure

Kromasil_amycoat_cellucoat_application_guide

Kromasil_amycoat_poster_2007

Kromasil_CelluCoat_Brochure

 

 

Kromasil Chiral Phases

Benefits:

  • Kromasil based silica
  • High chemical stability
  • High load-ability
  • Complementary phase selectivity
  • Application booklet

Kromasil DMB and TBB

Eka Chemicals AB offer the chiral phases Kromasil® DMB and Kromasil TBB, in which acylated N,N'-diallyl-Ltartardiamide network polymers have been covalently bound to Kromasil. Two key steps are involved in their synthesis. The chiral monomers are reacted with a multifunctional hydrosilane yielding a network polymer incorporating a bifunctional C2-symmetric chiral selector.

Kromasil Chiral synthesis

The chiral polymer is covalently bonded to functionalised Kromasil silica as shown below.

Kromasil chiral selection

 

Chiral Monomer

0,0’-bis(3.5-dimethylbenzoyl)-N,N-diallyl-L-tartardiamide

0,0’-bis(4 tert-butylbenzoyl)-N,N-diallyl-L-tartardiamide

Particle siz (um)

5,10

5,10

Surface area (m2/g)

340

340

Pore size A

100

100

Carbon load %

14.5

15.0

Nitrogen load %

0.6

0.6

 

Mechanical and Chemical Stability

Kromasil silica is one of the most mechanically stable HPLC silicas. The high chemical stability is due to the nature of the network polymer covalently bonded to the silica. Kromasil chiral phases can be used with most solvents and buffers in the eluent without degradation.

Loadability

The high loading capacity of Kromasil chiral columns is due to the high surface area of the silica and the high chiral ligand density. An example of the loading capacity in preparative scale is shown for naproxen as per below.

Kromasil naprox selection

 

Selectivity

Kromasil DMB and TBB separate a broad range of racemates and have been developed to complement each other in selectivity. For the separation of acidic racemates, it is recommended to start with the TBB phase. The table below shows a series of acidic, basic and neutral racemates evaluated on both phases under identical eluent conditions

Compound

Separation Factor (α)

DMB

TBB

Oxazepam 

1.13

1.35

Lorazepam

1.49

1.50

Baclaphenlactam

 1.32

1.53

Binaphtol 

2.75

1.23

Paraflutizide 

1.34

1.18

Bendroflumetiazide

1.32

1.24

Proglumide

1.26

1.56

Naproxen 

1.11

1.65

Ibuprofen 

1.12

1.51

Bupivacaine 

1.34

1.92

Tocainide 

1.12

1.33

 

 

 

Applications of Kromasil TBB and DMB chiral columns respectively

 

Kromasil_Chiral_Application Guide

Kromasil_aspects_when_designing_chiral_preparative_separations

Kromasil_chiral_DMB_and_TBB_datasheet

Kromasil_chiral_technical_evidence

Kromasil_strategic_approach_to_solving_chiral_separation_issues_by_hplc

 


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