Knowledge & Education

Gate to  Workshops and Purification/Separation Knowledge

A. Seminars and Workshops offered click here

B. What purification or separation method should I use to solve my problem?

  • Reversed Phase Liquid Chromatography (RP-LC)
  • Advanced RP-like methods
  • Hydrophilic Interaction Liquid Chromatography (HILIC)
  • Aqueous Normal Phase Liquid Chromatography (ANP-LC)
  • Normal Phase Liquid Chromatography (NP-LC)
  • Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)
  • Ion Exchange Chromatography (IEX)
  • Ion Chromatography (IC)
  • Hydrophobic Interaction Chromatography (HIC)
  • Affinity Chromatograph (AC)
  • Chiral Recognition Chromatography (CRC)
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  • Multi Mode Liquid Chromatography (MMLC)
  • Metabolomics
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  • Preparative & Process Scale Chromatography PSLC
  • Multi Dimensional Liquid Chromatography (MD-LC)
  • Kamasutra Inspired Technology (KIT)
  • Capillary Electrophoresis

C. Tools for purification, separation and chromatography specialists

D. What column or phase to I use?

Column / Phases Selector

Molecular Weight of my sample = <5000

My sample is soluble in organic solvents

Hexane soluble

Normal Phase (unbonded)

Normal Phase (bonded)

Methanol or Methanol Water soluble

Reversed Phase bonded phase

THF soluble

Gel permeation chromatography (small molecules)

My sample is soluble in water


Reversed Phase bonded phase


Reversed Phase bonded phase



Ion Chromatography





Molecular Weight of my sample = >5000

A. Synthetic Polymers

B. BioPolymers
B1. Fatty Acid based
B2. Saccaride based
B3. AminoAcid based
B4. Nucleotide based


My sample is soluble in organic solvents


Gel Permeation Chromatography

My sample is soluble in water


Gel Filtration Chromatography

Ion-Exchange (wide pore)

HILIC (wide pore)

Reversed Phase (wide pore)

Hydrophobic Interaction Chromatography

Affinity Chromatography


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