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Inertsil GL Science

Inertsil wide range of speciality columns.

Product range comprises:

Inertsil® is a high purity silica manufactured by GL Sciences, Japan. Inertsil ODS-2 and ODS-3 have been developed for searching applications involving acidic and basic molecules

Inertsil ODS-2 and ODS-3 have been developed for searching applications involving acidic and basic molecules

  • Inertsil phases: C4, C8, ODS, ODS-2, ODS-3, Phenyl
  • Particle sizes: 3.5 and 5Ám
  • Pore sizes: 100 & 150┼
  • Column i.d.: 1, 2.1, 3.2, 4.0, 4.6mm
  • Column length: 50, 100, 150, 250mm
  • Guard cartridges in various i.d. are available
  • Please contact us for 7.75, 10 or 21.2mm i. d. columns

Inertsil Phases

Inertsil
Phase

Functional
Group

Endcapped

Particle Size
(μm)

Pore Size (┼)

Surface Area
(m2/g)

Carbon Load
(%)

C4

Butyl

 Yes

 5

150

 320

7.5

C8 

 Octyl

Yes

5

150

320

10.5

ODS

Octadecyl

Yes

5

100

350

14

ODS-2    

Octadecyl

Yes

5

150

320

18.5

ODS-3

Octadecyl

Yes

 3, 5

100

 450

15

Phenyl

Phenyl

 Yes

 5

150

 320

10

  • Microbore (2.1mm i.d.) Columns
  • Medium Bore (3.0mm i.d.) Columns
  • Analytical (4.0mm i.d.) Columns
  • Analytical (4.6mm i.d.) Columns
  • Semi-Preparative and Preparative (7.75 - 21.2mm i.d.) Columns

 

Inertsil® 3 Series

  • Spherical porous silica
  • 3 and 5μm particle sizes
  • High purity silica base (99.999%)
  • Large surface area
  • Validated ODS-3 column

The Inertsil® 3 series of HPLC columns from GL Sciences is designed for the more challenging separations. The material is based on high purity 100┼ pore size silica and provides new selectivities to create a wide range of separation capabilities.

 

Inertsil Phase  

Endcapped

Particle Size (μm)

Pore Size (┼)

Surface Area (m2/g)

Carbon Load (%)

SIL

 

3, 5

100

450

 

ODS-3   

Yes

2, 3, 4, 5

100

450

15

ODS-3V 

Yes

5

100

450

15

ODS-P   

No

5

100

450

29

ODS-EP  

No

5

100

 450

9

Peptide C18

Yes

5

100

450

Proprietary

ODS-Sprint 

Yes

3, 5

100

450

 8.5

Sulfa C18 

Yes

3, 5

100

450

15

C8-3  

Yes

2, 3, 5

100

450

 9

Ph-3

No

 2, 3, 5

100

450

9.5

CN-3

No

 5

100

450

 14

NH2

No

5

100

450

8

Diol  

No

5

100

450

22

HILIC  

No

5

100

450

20

AX 

No

5

100

450

17

CX   

No

5

100

450

14

 

Why Inertsil ODS-3 is Super Inert?

A Superior Base Silica is used

The base material for Inertsil ODS-3 is a 99.999% pure silica. The 29Si NMR  spectrum of the silica is shown (Figure1A). The silanol groups, especially the geminal silanols, exhibit a very strong activity towards basic compounds. The small geminal peak is indicative of a superior base silica.

INERTFig 1a

ODS Modification is Complete

After ODS bonding, the magnitude of the isolated silanols is greatly decreased. Residual silanols can, however, still adsorb strongly basic compounds (Figure 1B).

INERT Fig 1b

Isolated Silanols are Completely Endcapped. After endcapping, the isolated silanols have been completely eliminated, thereby assuring excellent inertness and optimum performance of ODS-3 (Figure 1C).

INERT Fig 1c

 

Inertsil ODS-3V

A Validated Inertsil Column Inertsil ODS-3V is a highly efficient, validated HPLC column, developed to provide optimum separation for a wide range of acidic and basic compounds. Each lot of packing undergoes a series of critical analytical tests to confirm:

    1) the absence of surface silanols
    2) minimal metallic residues
    3) inertness to basic compounds
    4) reproducible separations

 

INERT Fig 2

In Figure 2 the separation of a series of antidepressant drugs demonstrates the inert nature of ODS-3.

Inertsil® ODS-P

  • Polymerically bonded C18
  • Improved separation of planar compounds
  • Useful for carotenoid analysis
  • Separation of the 16 EPA PAHs

Inertsil® ODS-P is a polymerically bonded phase which is densely bonded with octadecylsilane groups. It shows unique separation capabilities and offers an alternative selectivity to Inertsil ODS-3. Inertsil ODS-P is particularly suited to the separation of polycyclic aromatic hydrocarbons, and shows additional retentivity for planar compounds.

INERT Fig3

Figure 3 shows the separation of components from toad venom.

INERT Fig 4

Figure 4 shows the separation of terphenyl isomers on three types of ODS phases. On the polymeric Inertsil ODS-P phase, the planar p-terphenyl is retained longer than the nonplanar o- and m- isomers.

 

Inertsil ODS-EP

  • C18 with polar embedded group
  • Compatible with 100% aqueous eluents
  • Alternative selectivity to C18
  • Ideal for LC-MS

Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C18 group. This creates a stable phase, which is resistant to phase collapse in 100% aqueous eluents. It provides highly reproducible separations for acids and bases in organic eluents as well as acidified eluents typically used in LC-MS.

INERT Fig 5

 Figure 5 shows a comparison of the selectivity of Inertsil ODS-EP with that of a C18 phase.

 

Inertsil® ODS-Sprint

  • Balanced retention of polar and non-polar compounds
  • Compatible with 100% aqueous eluents
  • New Sprint HG column hardware
  • Low operating back pressure

Inertsil® ODS-Sprint is super base deactivated and optimally bonded to retain polar compounds without excessive retention of non-polar compounds. This phase shows excellent peak shapes for a wide range of polar analytes, including both basic and acidic compounds.

INERT Fig 6

 In Figure 6 the hydrophobic compounds are eluted in approximately half the time of Inertsil ODS-3, whilst maintaining retention of polar compounds. Inertsil ODS-Sprint operates at low back pressures, even at high flow rates, and shows excellent robustness and reproducibility. To complement this new phase, a new zero dead volume column hardware format has been developed, to help maximise the efficiency and peak shape obtained with Inertsil ODS-Sprint. The 20 x 2.1mm Sprint HG column combines sharp peaks with fast elution time and is ideal for high throughput LC-MS analyses.

Inertsil® Sulfa C18

Inertsil Sulfa C18 (3 and 5μm) is designed for the analysis of sulfa drugs, as shown in Figure 7. In addition, it is effective in the screening of synthetic antibacterial agents and residues in products of animal origin, as directed by the food safety investigative guideline

INERT Fig 7

 

Inertsil Peptide C18

The Inertsil Peptide C18 column is specifically suited to the analysis of peptide digests.

INERT Fig 8

Figure 8 shows the application of this column to the analysis of cytochrome C tryptic digest. Please contact Hichrom for ordering information for Inertsil Peptide C18.

Inertsil HILIC

  • Unique selectivity for polar compounds
  • High sensitivity LC-MS analysis
  • Low operating back pressure
  • Applications for amino acids, water soluble vitamins and drug metabolites

Inertsil HILIC is a propyl alkohol bonded silica phase, suitable for the analysis of polar compounds in the hydrophilic  interaction (HILIC) mode. It has also shown promise in SFC applications. Unlike traditional reverse phase materials, HILIC column retain highly polar compounds  with only smal amounts of water in the eluent. The high organic solvent composition of the eluent will lead to high sensitivity LC-MS analysis, due to decrease in ion suppression. It also results in low operating back pressure and the ability to run at high flow rates.

INERT Fig 9

Figure 9 shows the separation of a mixture of oligosaccarides on Inertsil HILIC

Inertsil HILIC has been used in the development of a fast and sensitive LC-MS/MS method for the analysis of melamine and cyanuric acid contaminants in pet food samples (See Fig 10)

INERT Fig 10

 

Inertsil Diol

  • Unique selectivity in both reversephase and normal phase mode
  • Applications for amino acids, water soluble vitamins and sugars

The Inertsil Diol phase is a dihydroxy propylbonded silica which can be used in both reverse phase and normal phase modes. The   is higher than of silica in normal phase mode

INERT Fig 11

Figure 11 shows the analysis of free amino acids on an Inertsil Diol column

 

Inertsil Ion-Exchange Phases

  • Silica based ion-exchange columns
  • High reproducibility
  • High efficiency
  • Exceptional stability and long column lifetime

Inertsil AX and CX are silica based anion and cation-exchange phases respectively, based on high purity silica.They show good reproducibility, exceptional stability and long column lifetime.

INERT Fig 12

Figure 12 shows the separation of nucleotides on Inertsil AX and Figure 13 shows the analysis of nucleic acid bases by cation-exchange chromatography on Inertsil CX.

Inertsil Ion-exchange Phases

 

AX

CX

Bonded Phase

Diethylaminopropyl

Propylbenzenesulphonyl

Particle Size (μm)

5

5

Pore Size (┼)

100

100

Surface Area (m2/g)

450

 450

Carbon Load (%) 

17

14

Endcapped

No

No

pH Range

2-7.5

2-7.5

 

INERT Fig 12

INERT Fig 13

Figure 12 shows the separation of nucleotides on Inertsil AX and Figure 13 shows the analysis of nucleic acid bases by cation-exchange chromatography on Inertsil CX.

Inertsil ODS-3 2μm

  • Ideal for high throughput analysis
  • Easy down-scaling from 5, 4 and 3μm phases
  • Use with conventional or high pressure HPLC instrumentation
  • Maximum pressure 50MPa (7200 psi)

Inertsil ODS-3 2μm is based on the same high purity silica as Inertsil ODS-3 3, 4 and 5μm phases. This means that high speed separations can be achieved without altering the analytical conditions or the selectivity. Advanced classification technologies for sizing silica gels has led to an ideal balance between theoretical plate number and column back pressure, enabling ultrafast analyses using existing HPLC systems. Fine particles are eliminated which results in less column frit clogging problems. A newly developed column joint is used to minimize the dead volume, resulting in sharp peaks for fast eluted samples.

INERT Fig 14

Figure 14 illustrates the stepwise down-sizing of a separation from a 5μm to a 2μm particle size column. In addition to reducing analysis time, the eluent consumption has also been reduced approximately six-fold on transferring from 5μm 150 x 4.6mm to 2μm 50 x 3.0mm column, reducing environmental impact and lowering solvent disposal costs.

 

Inertsil® 4 Series

A new series of high purity state-of-the-art Inertsil phases, showing similar retention and selectivity characteristics to Inertsil ODS-2, is now available. Inertsil ODS-4 and C8-4 columns are produced in 2, 3 and 5μm particle sizes and a wide range of column dimensions.

 

Inertsil Wide Pore Phases

  • High purity 300┼ silica phases
  • Excellent reproducibility
  • Good physical and chemical stability

The Inertsil WP300 series of columns has been developed for the analysis and purification of proteins and peptides. It currently consists of five phases, in column dimensions ranging from capillary to preparative.

 

INERT Fig 15

Figure 15 shows the separation of a polypeptide mixture on Inertsil WP300-C8.

 

Capillary and Nano Columns

  • Excellent efficiency and column-to-column reproducibility
  • Easy installation
  • Easy coupling with MS

GL Sciences produce a range of capillary and nano columns. The Capillary EX columns are available with internal diameters of 0.3, 0.5 and 0.7mm and the Capillary EX-Nano columns have internal diameters of 0.05, 0.075, 0.1 and 0.2mm. Most of the Inertsil 3 and Inertsil WP300 Series, 3 and 5μm phases, are available in capillary format.

INERT foto Cap

 

Bioptic AV-1 and AV-2

  • Glycoprotein bonded silica
  • Direct injection of plasma samples
  • Proteins elute in void volume
  • Large sample capacity
  • Chiral separations with Bioptic AV-1

Bioptic AV columns eliminate the need for lengthy deproteinization steps prior to sample injection on reversed phase columns. As a result of special bonded phase technology, the hydrophobic inner surface of the silica is shielded from proteins by a permanently bonded hydrophilic outer avidin layer. Plasma proteins are excluded and elute by size exclusion mechanisms in the void volume. Smaller drug analytes pass through the avidin layer and interact with the hydrophobic inner layer by a reversed-phase mechanism.

Bioptic AV-1 and AV-2 Phases

Particle Size

5μm

Particle Shape

Spherical

Recommended pH Range

 2 - 7.5

 

INERT Bioptiv

Bioptic AV-1 contains non-denatured avidin and a chiral centre, allowing separation of chiral drugs and metabolites.

Biotic AV-2 contains a denatured form of avidin and lacks chiral separating ability. Figure 16 shows the direct analysis of donepezil (E2020) enantiomers in plasma on Bioptic AV-1.

INERET Fig 16

 

Titansphere®

  • Titanium dioxide phase
  • Amphoteric ion-exchange properties
  • Stable at pH 2-12
  • Excellent for extraction of phosphopeptides

Available as capillary columns, cartridges, pipette tips and bulk material Selective Extraction of Phosphopeptides Titansphere has been found to be extremely effective in the selective isolation of phosphopeptides. Phosphopeptides, in particular those containing serine, threonine and tyrosine residues, are of interest in biochemistry and proteomics, but are frequently present in low abundance. Selective extraction of these phosphopeptides can be achieved under acidic conditions using Titansphere. They can then be desorbed in alkaline conditions, without dephosphorylation occurring. Titansphere is particularly useful in 2D chromatographic separations, where it is used as a precolumn or trapping column prior to on-line column switching to a reversed-phase or ion-exchange analytical column and UV or MS detection.

INERT Fig 17

Figure 17 shows the selective extraction of phosphopeptides from a tryptic digest of casein. Figure 17(a) represents the tryptic peptides analysed on Inertsil WP300-C8 alone, whereas Figure 17(b) represents the same sample with a Titansphere precolumn (hence trapping the phosphopeptides) and Inertsil WP300-C8. Figure 17(c) indicates the phosphopeptides extracted by Titansphere, reanalysed on Inertsil WP300-C8

Titansphere® consists of porous spherical particles of titanium dioxide, with a smooth and alkaline surface that displays amphoteric ion-exchange properties. Titansphere products are specifically designed for biological applications involving phosphopeptide extraction. Capillary and nano columns, extraction cartridges, bulk material and pipette tips can all be supplied.

Titansphere Phase

Nature of phase

Spherical TiO2

Particle Size (μm)

5, 10

Pore Size (┼)

100

Surface Area (m2/g)

100

pH Range

2 - 12

 

 

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