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MTAKT operates from two sites, - Imtakt Corporation co-founded by Itaru Yazawa is located in historic Kyoto, Japan and IMTAKT USA is located in Philadelphia, USA. Mr. Yazawa developed HPLC columns for YMC and instruments for Schimadzu for more than 20 years before founding Imtakt .
IMTAKT focusses on design, development and production of highly innovative and unique HPLC column for solving complex separation problems
They structure the Product range as follows:
Multi-Mode-Reverse Phase column to separate Anion, Cation, Neutral compounds
High resolution, non-porous 2um reverse phase columns for Peptides, Proteins, Bio- or Synthetic- Polymers
Wide-pore, low polarity reverse phase columns Protein
High resolution low pressure reverse phase columns with different selectivity
- Cadenza Range
- Cadenza CD-C18
- Cadenza 5CD-C18
- Cadenza CL-C18
- Cadenza 5Cl-C18
- Cadenza HS- C18
- Cadenza CW-C18
High resolution , traditional reverse phase columns
- Unison UK Range
- Unison UK - C18
- Unison UK - C8
- Unison UK - Pheny
- Unison UK - Silica
- Unison UK - Amino
Product Propertie
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Column Name
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Column Type
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USP Code
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Particle Size(µ)
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Pore Size (nm)
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Lengths (mm)
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Internal Diameters (mm)
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Scherzo SW-C18
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Reverse Phase - Normal Phase & Ion Exchange
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L1
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3
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13
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10 - 500
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0.075 - 28.0
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Scherzo SM-C18
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Reverse Phase - Normal Phase& Ion Exchange
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L1
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3
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13
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10 - 500
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0.075 - 28.0
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Scherzo SS-C18
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Reverse Phase - Normal Phase& Ion Exchange
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L1
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3
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13
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10 - 500
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0.075 - 28.0
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Presto FF-C18
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Reverse Phase
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L1
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2
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NA
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10 - 250
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0.075 - 4.6
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Intrada WP-RP
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Reverse Phase
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N/A
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3
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30
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10 -150
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075 - 10
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Cadenza CD-C18
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Reverse Phase
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L1
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3
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12
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10 - 500
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0.075 - 10.0
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Cadenza 5CD-C18
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Reverse Phase
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L1
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5
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12
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10 - 250
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0.075 - 28.0
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Cadenza CW-C18
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Reverse Phase
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L1
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3
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30
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10 - 500
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0.075 - 10.0
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Cadenza CL-C18
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Reverse Phase
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L1
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3
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12
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10 - 500
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0.075 - 10.0
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Cadenza HS-C18
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Reverse Phase
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L1
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3
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12
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10 - 500
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0.075 - 10.0
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Unison UK-C18
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Reverse Phase
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L1
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3
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13
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10 - 500
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0.075 - 10.0
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Unison UK-C8
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Reverse Phase
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L7
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3
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13
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10 - 500
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0.075 - 10.0
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Unison UK-Phenyl
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Reverse Phase
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L11
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3
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13
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10 - 500
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0.075 - 10.0
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Unison UK-Silica
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Normal Phase
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L3
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3
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13
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10 - 500
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0.075 - 10.0
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Unison UK-Amino
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Normal Phase & Ion Exchange
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L8
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3
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NA
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10 - 500
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0.075 - 10.0
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Unison US-C18
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Reverse Phase
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L1
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5
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13
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10 - 250
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0.075 - 28.0
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Scherzo Family
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Scherzo SW-C18
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Scherzo SM-C18
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Scherzo SS-C18
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Multi-Mode
Reversed Phase + Anion Exchange + Cation Exchange
Scherzo family columns can operate without adding an ion-pairing reagent which is required for conventional ODS columns. In addition, both anionic and cationic compounds are retained on this column. The hydrophobicity of the Scherzo family is similar to that of a conventional ODS column, so analysis of a compound in combination with a Unison UK-C18 column (conventional ODS phase) will be very effective for method development.
Applications
- Consists of a lower density of strong anionic and cationic ligands as well as C18 ligands.
- Simultaneous analysis of both cationic and anionic compounds in RP and Ion Exchange separation mode
- For polar compounds L
- C-MS compatible without using ion-pair reagents
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Large amount of strong ionic ligands loaded onto this ODS column. Effective for improved retention of zwitterions or weak ionic compounds
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Weak ionic ligands adequately loaded onto this ODS column. Designed for separation of basic / acidic compounds at neutral pH condition
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Low amount of strong ionic ligands loaded onto this ODS column. Effective for strong ionic compound elution or basic compounds with formic acid eluent
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Retention properties of multi-mode ODS, Scherzo family
You can find the best HPLC conditions by optimizing salt concentration or pH to improve retention / separation for ionic compounds, which is difficult on a conventional ODS column.
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For the Scherzo multi-mode ODS column, optimizing the salt concentration is as important as optimizing the organic solvent. In the left figure, retention of both anionic and cationic compounds are decreased when salt concentration is increased. At the same salt concentration, SSC18, which consists of a large amount of strong ionic ligands, retains strongly. SM-C18 , with weak ionic ligands has medium retention. And finally, SW-C18, which has a low amount of strong ionic ligands, shows the lowest retention.
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pH is also an important factor for the elution of ionic compounds. Retention will be dramatically changed between neutral pH (ammonium acetate or formate) and low pH (formic acid) conditions. SS-C18 and SW-C18 consist of strong ionic ligands and retention will be increased under low pH conditions. On the other hand, SM-C18 consists of weak ion ligands which cannot ionize at a low pH -therefore, retention of basic compounds will be decreased due to a decrease in ionization capacity at low pH.
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Presto FF-C18
Features
- World's first non-porous high resolution ODS column.
- Non-Porous 2 µm ODS-Silica
- Reversed-phase mode for polymers up to 30 MDa
- Separates almost any polymer including bio-polymers and synthetic polymers
- Amazing number of peaks for peptides and proteins
- Different selectivity from porous ODS columns
- 250mm length high-resolution column with 2um particle
- High-efficiency with low flow rate compatible with conventional HPLC systems
Applications
- polyethylene glycol (ca.20 kDa)
- DNA, (ca. 30 MDa)
- Monoclonal IgG (mAb, ca.150 kDa)
- Hyaluronic acid (ca. 1 MDa)
- Polyvinylpyrrolidone (ca.50 kDa)
- Very useful for protein analysis especcially peptide mapping or IgG (mAb) as follows:
- Recommended condition are ....
1) Lower flow rate than porous columns
2) Shallow gradient like several %B in 120min
3) Do not use single pump (low pressure) gradient, but binary pump with T-union (no mixer) gradient is recommended
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Examples
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Amazing separations for many molecular weights from peptides to proteins. Presto FF-C18, a non-porous ODS HPLC column, performs exceptionally well with peptide separations. HPLC and LC-MS analysis of a small amount of peptides is important for proteomics. Presto FF-C18 can achieve twice as many peaks as conventional porous ODS columns. Non-porous ODS Presto FF-C18 may change the proteomics field dramatically.
A: water /TFA = 100 /0.1, B: acetonitrile /TFA = 100 /0.1, 50 deg.C, 220nm , Tryptic digest of alpha-casein, 5uL, Presto FF-C18 (2um), 250 x 4.6 mm, 1-35%B (0-150min), 0.4mL/min (25MPa), Porous ODS (3um), 250 x 4.6 mm, 5-45% B (0-150min), 1mL/min (14MPa),
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Immunoglobulin G (IgG), a large protein (MW ca. 150kDa), can exhibit poor peak shape when injected onto porous RP columns, due to high mass transfer resistance. Non-porous ODS Presto FF-C18 can provide improved peak shape and recognition between mAb (same amino acid sequence) and polyclonal antibody (different amino acid sequence). Presto FF-C18 may change the antibody separation field, which has had difficulty using traditional porous columns.
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Tech Brochure Presto FF C18
Cadenza CD C18 vs Presto FF C18
Intrada WP-RP
Features:
- Reversed-Phase Column for Polymer Separation
- Reversed-phase tailored with a 30nm pore size
- Optimal for the separation of proteins and other large molecules
- Superior Resolution Column with 3um Particles
- High resolution 3um Silica is used
- Radically improved column efficiency compared with conventional 5um columns
- Optimal Structure Polarity for Faster Polymer Elution
- Uses a newly developed reversed-phase ligand
- Highly hydrophobic polymer elution made possible by optimal surface polarity
- Key specifications: 3um particle size, 30nm pore size, special ligand for reversed-phase, polymeric end- capping
Brochure Intrada
High Resolution Separation of 111 Proteins ( 9-225 kDa )
New proteomic analytical method called the fluorescent labeled protein method using Intrada WP-RP (Courtesy of Professor Emeritus Imai, University of Tokyo, Japan). The 3um particular, 500mm column provides the ability to separate large numbers of proteins.
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Cadenza Family
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Cadenza CD-C18
Molecular recognition
Multicomponent separation
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Applications
- For superior high throgh put analysis
- High resolution,
- Low pressure,
- Best with hydrophobic compounds,
- Molecular recognition, Multicomponant separation
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Cadenza 5CD-C18
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Applications
- High resolution,
- pH stable,
- Best with hydrophobic compounds,
- Molecular recognition, Multicompenant separation
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Cadenza CL-C18
Cadenza 5CL-C18
Contrast CD-C18
Silanols regulation
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Applications
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Cadenza HS-C18
Serum/plasma direct injection
Different selectivity
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Applications
- Serum/plasma direct injection,
- Different selectivity
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Cadenza CW-C18
High-throughput
Basic compounds
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Applications
Excellent peak shape for basic drug compounds and antibiotics, High- through put, Low pressure
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High Through Put Analysis
High Through Put High Pressure Brochure Cadenza CD-C18
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Unison UK - C18
Features
Applications
- Best with polar compounds,
- Separation balance,
- No phase collapse with 100% aqueous eluent
Unison US - C18
Features
Applications
- Best with polar compounds,
- Separation balance,
- No phase collapse with 100% aqueous eluent
Unison UK - C8
Features
Applications
- Best with polar compounds and basic compounds. ,
- No phase collapse with 100% aqueous eluent
Unison UK - Phenyl
Features
Applications
- High resolution,
- Low pressure,
- Best with hydrophobic compounds,
- Molecular recognition, Multicompenant separation
Unison UK - Silica
Features
Applications
- Aqueous to non-aqueous Normal phase
Unison UK - Amino
Features
Applications
- Revolutionary aqueous durability for aminopropyl phase,
- Aqueous to non-aqueous Normal Phase Separation
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