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Hichrom Columns

Hichrom formed 1978 has grown to become the largest HPLC column packer in the world.

Hichrom has developed in-house a range of specific HPLC columns and acquired a number of famous brands as follows:


Hichrom C8 and C18 Columns

Features:

  • Ultra pure base deactivated porous silica
  • Suitable for acidic, basic and neutral molecules
  • 3.5 and 5 um particle size with column efficiencies of > 150 000 plates / meter respectively 90 000 plates / meter
  • Excellent  batch to batch reproducibility
  • Extended column lifetime due to advanced bonding technology
  • High bonding stability
  • Professionally packed columns
  • All columns are supplies with a test Chromatogram and Batch Validation Certificate

 

Hichrom RPB

Features:

  • High purity base deactivated silica
  • 3.5 , 5 and 10 um particle sizes
  • Unique C8/C18 multi-alkyl phases with exhaustive endcapping
  • 3,5 um > 145 000 plates / meter
  • 5 um > 90 000 plates / meter
  • 10 um > 50 000 plates / meterSuitable for LC-MS and Prep applications
  • Competitively priced

Column

Functional Group

Endcapped

Particle size um

Pore Size A

Surface Area m2/g

pH Stability

Carbon Load %

Hichrom RPB

C8/C18 multi alkyl

yes

3,5 ,5,10

110

340

 

14

 

Hichrom PAH2

Features:

  • Optimised for EPA Method 610
  • Excellent separation
  • Polkynuclear aromatic hydrocarbon (PAHs) are important environmental pollutants due to their ubiquitous presence and carcinogenicity
  • These compound have to be analysed routinely
  • PAH2 is based on alkyl bonded silica with high carbon loading for separating 16 priority pollutants in just 30 minutes
  • PAH2  Columns are available in 2.1 mm, 3.2 mm and 4.6 mm id
  • The particle size is 5 um , Pore size is 150 A and the material is end-capped

 

Hichrom Chiral Column

Features:

  • Chiral Prikle type
  • Unique covalent bonding
  • Enantiomer order elution inversion
  • Two types of stationary phase. With Chira-Chrom 1 the OH group of silica has been modified by a nitrobenzoyl derivative of an optically active  amino acid. In Chira Chrom 2 a chiral dinitrophenyltartramide moiety is bonded to the silica surface through a propyl spacer group.
  • Hichrom high efficiency
  • By substituting L-phenylglycine for D-phenylglycine the order of elution of the chiral peaks can be reversed
  • Competitively priced

Specificatin

Hichrom

Chiral Type

Particle size um

Endcapped

D-Phenylglycine

Chira-chrom 1

5

no

L-Phenylglycine

Chira-chrom 1

5

no

DL Phenylglycine

Chira-chrom 1

5

no

L- Leucine

Chira-chrom 1

5

no

Dinitrophenyltartramide

Chira-chrom 2

5

no

 

Partisil

Partisil was one of the first commercial stationary phase available from Whatman and since March 2012 acquired by Hichrom. It is a irregular silica. A

  • Acquired by Hichrom from Whatman, a division of GE Healthcare in March 2012
  • Choice of spherical or irregular silica
  • large surface area gives it a high loading capacity.
  • Wide range of surface chemistries available
  • Manufacturing facilities accredited to both ISO9001 (Quality) and ISO14001 (Environmental) standards.

HICH partisil

Features

  • Partisil ion-exchange materials are still widely used today.
  • Partisil ODS3 shows similar HPLC performance to Waters ÁBondapak.
  • Partisil phases: Silica, C8, ODS, ODS2, ODS3, PAC, SAX, SCX
  • Particle sizes: 5 & 10Ám
  • Pore size: 85┼
  • Column i.d.: for 1 & 2.1 and 4mm i.d. please contact us for further details.  3.2 and 4.6mm i.d. area available
  • Column length: 50, 150, 250mm
  • Guard cartridges in various id. are available
  • All columns are available from stock for immediate delivery

Partisil Phase 

Functional Group

Particle Size (μm)

Silica

 -

5,10

C8

 Octyl

5,10

ODS 

Octadecyl

10

ODS2

Octadecyl

 10

ODS3 

Octadecyl

5,10

PAC

Amino-cyano

5,10

SAX 

Tetramethyl Ammonium

5,10

SCX 

Sulphonic Acid

5,10

 

Partisphere

  • Spherical 5μm porous silica
  • Unique void-sealing cartridge hardware
  • Convenient and easy to use - hand tightened fittings
  • Increased column lifetime

Partisphere was one of the first commercially available spherical silicas, and continues to provide reproducible, high efficiency separations. Partisphere columns are available in a wide range of surface chemistries, including a PFP  pentafluorophenyl (TAC-1) column

Partisphere Phase

Functional Group

Particle Size (μm)

Silica

-

 5

C8

 Octyl

5

C18 

Octadecyl

5

PAC

Amino-cyano

5

SAX

 Tetramethyl Ammonium

5

SCX 

Sulphonic Acid

5

TAC-1

Pentafluorophenyl (PFP)

 5

WAX (MAX-1) 

Proprietary

5

Unique Void-Sealing Cartridge System Partisphere columns are available in a unique void sealing (WVS) hardware. If a void eventually forms at the top of the column bed, simply hand tighten the inlet fitting to move the frit assembly downwards and recompress the packed bed, thus removing the void and restoring column efficiency. Large knurled end fittings allow ready hand tightening of the system. All WVS cartridge columns are shipped without end fittings and require end fitting kit p/n 4631-1001 – this item can be interchanged with additional WVS columns.

Partisphere Guard Cartridge System Additional installation of the unique WVS guard cartridge holder (p/n 4631-1003) allows the use of guard cartridges

HICHpartisphere

 

Ultrasphere

Ultrasphere was made some years ago by Beckman Coulter and taken over by Hichrom. It is a classical silica and boast a high performance reputation

Hichrom Beckmann

Features

  • Spherical porous silica
  • 3 and 5 um particle sizes
  • Specific ion-pair reverse-phase  column
  • widely referenced silica
  • Available in all standard sizes

Ultraspher

Particle Size (um)

Endcapped

Pore Size A

Silica

5

no

80

C8

3 & 5

Yes

80

ODS

3 & 5

Yes

80

CN

5

Yes

80

IP (C18 ion-pair column)

5

Yes

80

WHATMAN PREPSCALETM APM MEDIA

  • Irregular silica Advanced Performance Medium
  • Excellent chromatographic performance
  • Narrow particle size distribution
  • Large scale process applications
  • Customised media and chemistries

The Whatman PrepScale™ APM range of HPLC media has been developed specifically for large scale process applications for pharmaceutical, food, natural product and biotechnology industries. This Advanced Performance Medium is based on an improved base silica using improved bonding chemistries. However, it still retains the economic advantages of irregular silica compared to spherical.

Product

 Bonding Chemistry

Endcapped

Pore Size (┼)

Particle Size (μm)

PrepScale APM C8

Octyl

 Yes

60

10, 30

PrepScale APM C8

Octyl

Yes

150

10, 30

PrepScale APM C18

 Octadecyl

Yes

60

10, 30

PrepScale APM C18

Octadecyl

 Yes

150

10, 30

PrepScale APM Phenyl

Phenyl

Yes

60

 10, 30

PrepScale APM Phenyl

 Phenyl

Yes

 150

 10, 30

PrepScale APM Silica

 

 

60

  10, 30

PrepScale APM Silica

 

 

150

10, 30

Excellent Chromatographic Performance PrepScale APM media show excellent chromatographic performance compared to other spherical or irregular media. This is a result of narrower particle size distribution using state-of-the-art grinding and size classification technologies.

Particle Size Classification

The PrepScale APM 10 and 30μm media were developed to ensure that the size distribution of the silica particles is as narrow as possible (see Figure 1). This results in the production of columns with greater efficiencies and which show significantly lower back pressures, due to the absence of fines.

HICH whatparticle

Applications

Whatman APM media perform exceptionally well against competitor irregular silicas, but also compare favourably with the best spherical silicas. Figure 2 shows the separation of 7.5μg of each of five peptides on a 250 x 4.6mm i.d. column of Whatman PrepScale APM 10/150 C18.

HICH protein sep

Customised Media

For more stringent separations, Whatman are able to customize a matrix to fit specific requirements. A custom silica can be manufactured with the optimum particle size and distribution to maximize resolution and operating conditions. The chain length of the functional group can be varied to optimize selectivity for any separation. Attaching different functional groups allows for separations to be performed in the various modes of chromatographic operation (reversed phase, ion-exchange, normal-phase, affinity etc.).

 

 

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