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Silicahydride based HPLC Columns with very interesting performance properties
MicroSolv Technology Corporation located in Eatontown, NJ 07724, USA designs, produces and sells consumables and equipment for separation and purification sciences including::
- Unique Autosampler vials based on RSA Glass
- Unique HPLC Columns
We represent Microsolv Technology in the German speaking countries.
The HPLC Column product range is structured as follows
Cogent TYPE-C Silica Columns
Application selector
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| Independent scientific justification for C-Type Silica hydride stationary phases | | Prof. Pavel Jandera from University of Pardubice has long been considered one of the world leader in Chromatography. He has done a independent study of Cogent Type C HPLC Columns. Click to download | |
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Professor Jandera article on TYPE-C HPLC Columns 2012 Journal of Chrom A
Cogent Type B Silica Columns
Chiral Columns
High Performance Reverse phase columns based on type B Silica
Longer Chain, Hydrophobic Columns
Why should Chromatographer evaluate Type C-Silica based columns?
HPLC column technology based on ordinary silica has been extremely reliable since its development in the early 1970’s. Using organ-silanes and high purity silica particles to make columns, continual improvements to HPLC columns were made until the late 1990’s when the development slowed to almost a halt. Limitations due to the silica surface chemistry have become routinely accepted. Often under the new analytical demands “work-arounds” have been required when HPLC simply did not produce the desired results. To gain speed of analysis, sub 2 micron particles using standard HPLC column chemistry evolved into UHPLC. The run times have been incredibly reduced but these columns lack robustness, selectivity power and require time to equilibrate between runs. This results in faster run times but not robust methods with orthogonality and the cycle time from injection to injection is not rapid enough. This leaves the chromatography community still searching for fast technology that lowers the cost per analysis and method development.
Cogent Solution
Cogent TYPE-C Silica columns use a more modern approach to column chemistry than other HPLC columns. Using high purity silica particles with a surface chemistry that does not retain and hold water, these HPLC columns offer all the benefits of your favorite columns but have many additional features that help laboratories “do more with less”.
The surface of the TYPE-C™ columns are populated with silica hydride functional groups instead of silanols. This makes the particle slightly hydrophobic and will adsorb and desorb solvents much more easily than ordinary silica. Using proprietary bonding technology, the Type C™ silica-hydride columns have also been modified with C8, C18, Cholesterol, and Phenyl with a slightly modified hydride surface for additional phase selectivities.
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Benefits of Cogent Silica hydride Technology
Benefit 1 : Faster turn-around-time between runs. Most HPLC columns require 15-20 columns volumes to equilibrate. TYPE-C columns only require 1-2 column volumes to save you time, solvents and cost per analysis.
Benefit 2 : Easy method development. Retain both polar and non polar compounds on the same column and in less than 60 minutes identify the best column for your method optimization. Most methods developed on TYPE-C columns use the same mobile phase even when changing to different bonded phases.
Benefit 3 : Extended column lifetime and less lifetime failures and investigations. Direct silicon carbon bonds and the lack of a need for end capping makes these columns last 10-15 times longer than columns based on older silica technology that requires end capping.
Benefit 4 : Selectivity enhancements. All Type C column phases can be used in any of the (3) modes of chromatography. Reverse Phase, Normal Phase or Aqueous Normal phase can be used including switching between modes without hysteresis or damage to the column.
Benefit 5 : Easy to use. Simple singular mobile phases such as acetonitrile and water with acid or base can usually separate most compounds. No PIC reagents are needed. Use our fast “Quick Start” method to easily determine the best chromatographic mode to deploy for unknown polar or non polar compounds
Benefit 6 : Save time and money, do more with less. The initial cost of column acquisition is similar to other market leaders, but the reliability, precision, fast equilibration, savings in personnel and instrument time, solvents, extended column lifetime and low column failure rate, gives incredible overall column life-time savings.
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Eluent selection strategy
- A wide range of solvents can be used with Cogent TYPE-C Silica™ Based HPLC Columns..
- Selecting which TYPE-C column to use (C18, C8, Cholesterol or Silica-C) will be determined by your compounds of interest. Mixtures that will be highly polar and do not contain non polar compounds might be better suited by the Silica-C where compounds with both polar and non polar might be better suited to the Bidentate C8, C18 or UDC Cholesterol.
- Step 1. After you have properly installed and conditioned the column it is a good idea to start with a typical reverse phase gradient run. We suggest starting with an acidified mobile phase of Water as component A and Acetonitrile as component B. Acidify both components with up to 0.5% of an acid such as Formic, Methyl Phosphonic or Acetic Acid. If you are not using LC-MS, TFA (up to 0.1%) is another good candidate.
- Step 2. Run about 6 column volumes of the mobile phase in Step 1 at 95% Water.
- Step 3. Set up your instrument to run a shallow gradient from 95% Water (A) to 40% Water (A) over 20 minutes on a 75mm long column. For longer or shorter columns, increase the gradient time proportionally.
- This long and shallow gradient will be very beneficial for determining the optimal gradient or isocratic method to run your mixture with later. For sharper peaks and less retention, run a shorter (Steeper) gradient from the same starting points to end points.
- Step 4. Equilibrate the column by running 100% Acetonitrile for approximately 2 minutes for the 75mm long column.
- Step 5. Set up your instrument to run a shallow gradient using the same mobile phase to run from 90% Acetonitrile (B) to 40% Acetonitrile(B) over 20 minutes for a 75mm long column. For longer columns increase the gradient time proportionally.
- This long and shallow gradient will be very beneficial for determining the optimal gradient or isocratic method to run your mixture with later. For sharper peaks and less retention, run a shorter (Steeper) gradient from the same starting points to end points.
- Step 6. Evaluate both gradient runs for retention time, peak shape and elution order. Since analyte retention on these columns is compound and method specific some compounds may not retain in Step 3 (Reverse Phase) and some may not retain in Step 5 (Aqueous Normal Phase). However, one column could produce an isocratic run which retains both polar and non polar compounds.
- Note: The Cogent Bidentate C8, C18 and UDC-Cholesterol columns have a unique quality in that they may retain polar compounds not retained on other columns while run at 100% Water without loss of retention with continued use. You could insert an isocratic run at 100% acidified Water after Step 3 and before Step 4. Optimizing Suggestions:
- If you do not find satisfactory results or once you have established some retention and selectivity, you may need to optimize the method for your specific compounds. We have listed some suggestions below that will offer some ideas. If you are not satisfied with the results, please contact our technical support team.
For some organic acids that do not retain at low pH in an ANP mobile phase, you can try a high pH ANP mobile phase for retention. A rule of thumb is that for acids you would want to be from 1-2 pH units above the pKa. Some examples of additives that may work for you are Ammonium Acetate, Ammonia, Sodium Acetate, Ammonium Formate or Sodium Formate.
- The choice of solvents that you make for ANP will have different resolving powers for Acids and Bases. Acetone, Acetonitrile, THF, Ethanol, Acetonitrile/Methanol, Methanol and then Water will have a descending strength of retention in ANP.
- All Cogent TYPE-C columns all have the ability to perform in ANP. Depending on your sample mixture and matrix, different columns will produce different peak shapes, retention power and selectivity. Ranging from Silica-C™, Bidentate C8, UDC-Cholesterol and Bidentate C18, the amount of reverse phase will increase in this order.
- Equilibration between gradient runs. Although the Cogent TYPE-C Columns all equilibrate quickly, the mobile phase solvents you are using will determine how much equilibration time you may need between runs. The hydride surfaces of these columns have a preference for Methanol and therefore, it may take longer to equilibrate if methanol is used in a gradient end and not in the beginning. To equilibrate, use the starting point of your gradient as the solvent composition for equilibration.
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Cogent UDC Cholesterol
Structure Features
- The Cogent UDC-Cholesterol™ is bonded directly to the TYPE-C™ silica hydride surface with a direct silicon-carbon bond.
- The many advanced features of the Cogent UDC-Cholesterol column produces a very stable, efficient and unique HPLC column that operates in reverse phase, normal phase and or aqueous normal phase and will solve many difficult separation problems for you
Applications:
4-Andrestene-3, 17-dione, Adrenosterone, Anthracene, Atropine Determination, Benzene
Benzopyran,Biological Fluids, Choline, Corticosterone, Cytidine-R1, Cytidine-R2, Cytidine-R3, Estradiol, Estrone, Ethinyl Estradiol, ethyl-Benzene, Glyburide, Isopropyl Benzene, Metformin, Norgesterel, Nucleosides, Prednisolone, Phytonadione Isomers, Prodrugs, Progesterone, Quinolinedione-R1, Quinolinedione-R2, Saccharin, Steroid Mixture (1), Series# 1C, Steroid Mixture (2), Series# 2D, tert-butylBenzene, Tobramycin, Vitamin K1
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Cogent Bidentate C18
Structure
Features
- Reduce Column Budget By 90% ,
- Reduce Cost of Acquisition
- Reduce Cost of Ownership
- Using our proprietary bonding technology, the Cogent Bidentate C18™ is bonded directly to the TYPE-C™ silica surface with TWO (2) separate points of attachment. This combination gives very stable, efficient and unique HPLC columns that operate in Reverse Phase, Normal Phase and Aqueous Normal Phase.
Applications:
100% Aqueous - Guanidine, Acetaminophen, Acetaminophen (BAC), Acetaminophen by HPLC (RP), Acetic Acid, Acetylsalicylic Acid, Aminoacetophenone, Amino-Caproic Acid, Amoxicillin, USP (RP), Aspirin, Aspirin, Atorvastatin, Atorvastatin by LCMS, Benzoic Acid, Benzonatate, Benzophenone, beta-Carotene, Butalbital (BAC), Caffeine, Caffeine (BAC), Carbohydrates, Carisoprodol, Carotenoids, CARVONE, CARVONE, CARVONE - Normal Phase, Catechin (RP), Catecholamines, Cellotriose, Claratin Standard/Loratadine, Cleaning Validation w/ Guanidine, Closely Related Compounds, Corticosterone, Diovan®, Dipyridamole, Drug Metabolite (RP), Epicatechin (RP), Folic Acid, Formic Acid, Fumaric Acid, Furazolidone, Furosemide USP, Gallic Acid (RP), Guanidine - 100% Aqueous, Guanidine - Cleaning Validation, Hydrochlorothiazide (HCT), Inverse Gradient, Isomaltriose, Lamotrigine, Lasix™, Lipitor™, Loratadine, Loratadine (2), Loratadine (Claratin™), Loratadine - Normal Phase, Maltotriose, Meprobamate, Meprobamate, Metanephrine, Metformin & Glyburide, Methotrexate, Norgestrel, Normal Phase Made Easier, Normal Phase Prep & Purity, Normetanephrine, Organic Acids, Organic Acids (3), Series# 2B, Rapid Development, Oxalic Acid, Panose, Paracetamol, Phenolic Compounds, Polar & NonPolar Compounds, Polycyclic Aromatic Hydrocarbons (PAH), Polyphenols (RP), Prednisolone, Prednisone, Progesterone, Propionic Acid, Pyridoxine, Riboflavin, Salicylic Acid, Salicylic Acid, Separation by Functionality on a C18, Simvastatin, Sodium Azide, Sulfonamide by LCMS, Tetracycline, Thiamine Nitrate, Uracil, Urea (RP), Valsartan, Vitamins B1, B3, B2, B6, B11, Warfarin, Zeaxanthin
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Cogent Bidentate C8
Stucture
Silica hydride with C 8 ligand
Features
- Great Started Column for ANP
- Polar & Non Polar Analytes
- For Complex Mixtures
- Using our proprietary bonding technology, the Cogent Bidentate C8 is bonded directly to the TYPE-C silica surface with TWO (2) separate points of attachment. This combination gives very stable, efficient and unique HPLC columns that operate in 3 different domains: Reverse Phase, Normal Phase and Aqueous Normal Phase. A very powerful tool for every serious separations lab. See Bidentate C18 Pages for More Information on Selectivity and other features of the Bidentate C8 that can be run in Reverse, Normal or Aqueous Reverse Phase due to minimal silanol groups.
Applications:
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Cogent BidentateC8 300
Stucture
Features
- Using our proprietary bonding technology, the Cogent Bidentate C8 300 has a C8 chain bonded directly to the 5um, 300A TYPE-C™ silica surface with TWO (2) separate points of attachment resulting in silcon-carbon. Since there are no siloxane bonds, the bonded does not hydrolyze in acidic conditions. Extremely low pH is possible with this column.
- This column is a very stable, efficient and unique HPLC column for macro molecules. Reverse Phase, Normal Phase and Aqueous Normal Phase are all possible with the great wide pore column.
- A very powerful tool for bio separations labs working with large proteins, peptides, polysaccharides or tryptic digests looking for longer column life and improved precision in results
Applications:
a1-acid-glycoprotein, Sheep HPLC, a2-hs-glycoprotein, human plasma HPLC, Angiotensin II HPLC, Apomyoglobin HPLC, Cytochrome C HPLC, Cytochrome C HPLC, Glycoprotein Standard Bovine Serum HPLC, Gly-Tyr HPLC, Holo-transferrin HPLC, Human Plasma HPLC, Leucine enkephalin HPLC, Methionine enkephalin HPLC, Monoclonal anti-a1-glycoprotein HPLC, Peptide Standard Mixture HPLC, Ribonuclease A HPLC, Val-Tyr-Val HPLC,
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Cogent Silica-C
Stucture
Features
- Totally Inorganic
- Will Not Adsorb Water
- Use for Prep or Analytical
- The Cogent Silica-C is HPLC grade silica with a silicon hydride surface (silica hydride). Made completely free of carbon, this unique stationary phase will adsorb and desorb solvents commonly used in mobile phases very differently from ordinary silica resulting in fantastic benefits to the chromatographer.
- Since these columns have virtually no silanols remaining (less than 2%), they do not have a strong association with water and other solvents and will not have the expected hydration shell of other silica based columns. This allows you to use them for normal phase HPLC without having to concern yourself with the moisture content of the solvents.
- Excellent choice for normal phase and preparative chromatography because they produce a very unique selectivity are extremely stable for long column life and can be used to retain some polar compounds making it a favorite of many doing LCMS and medicinal chemistry.
Applications:
Aromatic Amino Acids, Phenolic Compounds, Phenylglycine HPLC, Reproducibility of Run to Run HPLC,
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Cogent Diamond Hydride
Stucture
Features
- Excellent for Extreme Polarity
- Slightly Hydrophobic
- Better Lot to Lot Consistency, Non Modified or Bonded, isocratic mobile phases more effective, less need for mobile phase additives.
- Extremely low to no silanol activity.
- These useful HPLC columns have a silicon hydride surface similar to other TYPE-C phases but the uniqueness of Diamond Hydride™ is that they have a very small amount of carbon on the surface that adjusts the hydrophobicity to a small but important level.
- Popular choice of scientists working in metabolomics with LCMS for compounds such as amino acids, organic acids, carbohydrates and very polar small molecules. Precise methods are easily developed even with complex sample matrices inlcuding biological fluids such as plasma, urine, saliva and other bio matrices.
Applications:
See Article: Simultaneous separation of hydrophobic and hydrophilic peptides with Silica hydride stationary phases
Analysis of cycloserine and related compounds
3',3'-Diaminobenzidine (DAB), 3-Hydroxytryptamine, 4-Dimethylaminopyridine, 4-Hydroxyproline, 4-Hydroxyproline, Aconitic Acid (Trans), Adenine, Adenosine, Adenosine-3,5-cyclic monophosphate, Adenosine-5- monophosphate (AMP), Adenosine-5- triphosphate (ATP), ADP - Glucose, Alkaloids in Citrus Juice, Amino Acids - LCMS, (ASMS Poster), Amino Acids - Easy method, Amino Acids - Underivatized,Amino Acids (2) - Underivatized, Amoxicillin, orthogonal method to USP, Anatoxin-a, Anatoxin-a (2), Arginine (ANP), Arginine (L), Ascorbic Acid, Asparagine (L), Aspartic Acid (L), Aspartic Acid (L), ATP, ATP & AMP, Betaine, Beta-Alanine, Bile Acids in Urine, Catalyst: DMAP, Cellular Extracts, CDP - Glucose, Chenodeoxycholic Acid, Chenodeoxycholic Acid, Chlorhexidine, Chlormequat, Choline, cis Aconitic Acids, Citric Acids, Citric Acid (by ANP), Creatine, Creatine, Cyanoguanidine, Cyanoguanidine, Cyanuric Acid, Diaminobenzidine, Diphenhydramine HCl , D,L - Asparagine, D,L - Ornithine, DMAP, Domoic Acid, Domoic Acid (LCMS), Domoic Acid in Seafood, Folic Acid (by ANP), Folic Acid in Cereals (ANP), Fumaric Acids, Fumaric Acid (by ANP), Fumaric Acid (Isobaric), Gabapentin, Galactose-1-phosphate, Glucose, Glucose - ADP, Glucose - CDP, Glucose - Na Adducts, Glucose - UDP, Glutamic Acid (L), Glutamine (D,L), Glutaric Acid, Glyphosate, Guanidine, Guanine, Guanine, Guanosine-5'-triphosphate, Hexanolamine - UDP, Hexanolamine - UDP, Histidine (ANP), Human Fluids, Hydroxy Cinnamic Acid (Trans), Hydroxytryptamine, Hypoxanthine, Hypoxanthine, Isobaric Compounds, Isoleucine, Leucine, L-Glutamic Acid, L-Methionine, Lysine (ANP), Lysine (D,L), Maleic Acids, Maleic Acids (by ANP), Maleic Acid (by ANP), Maleic Acid (Isobaric), Melamine, Mepiquat, Metabolites, Human Urine, Metabolites in Urine, Metformin, Metformin HCL, Methionine (L), Methylsuccinic Acid, N-methyltyramine, Nucleobases, Nucleotides, Purine, Nucleotide Bases, Nucleotides (ANP), Nucleotides (Proprietary), Organic Acids, Organic Acids (by ANP), Ornithine, Oxaloacetic Acids, Oxaloactic Acid, Proline, Propantheline Bromide, Purine Base & Purine Nucleoside, Purine Nucleotides (ANP), Sarcosine, Serotonin, Serotonin Metabolites, Shikimic Acid standards, Shikimic Acid in red wine, Sorbitol, Succinic Acid, Sucrose Acid, Sugar Nucleotides by ANP, Synephrine, Thymine, trans-4-Hydroxy-L-Proline, Trans Hydroxy Cinnamic Acid, Tryptamine, Tryptamine Hydrochloride, Tryptamine in Juice , UDP - Galactose, UDP - Galactose, UDP - Glucose, UDP - Glucose, UDP - Hexanolamine, Uric Acid, Uracil, Uridine 5'-diphosphate (Galactose), Uridine 5'-diphosphate (Glucose), Uridine 5'-diphosphate (UDP), Uridine 5'-diphosphate (UDP), Xanthine, Xanthosine-5'-monophosphate
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Cogent Phenyl Hydride
Features
- Excellent for Aromatic Compounds
- Use in RP or ANP
- These useful HPLC columns have a silicon hydride surface similar to other TYPE-C phases but the uniqueness of Phenyl Hydride™ is that they have unique selectivity for compounds with aromatic rings with all the TYPE-C silica benefits. These columns can be used in RP, ANP or NP and changed back and forth without damage to the columns.
- Popular choice of labs working with small peptides, sulfonyl, azide and other aromatic compounds for the long term stability of these columns and quick precision from run to run. Used also with LCMS because there is no end-capping that will bleed into your MS as with most other phenyl columns.
- Direct silicon carbon bonds and the hydride surface give these columns extremely long life times compared to other phenyl columns based on ordinary silica.
- Increase your through put and “do more with less” using the Cogent Phenyl Hydride HPLC columns.
Applications:
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Metabolomics Kit
Who would be interestin in a metabolomic kit?
- 1. Anyone working with Metabolites
- 2. Anyone working with polar compounds
- 3. Anyone who is looking to increase throughput in LCMS
- 4. Anyone Interested in learning new ways to do HPLC
HPLC Column Kit for Metabolomics!
- If you are frustrated with analysis of hydrophilic metabolites from serum, plasma, urine, saliva or any biological fluid, this column kit is for you. Cogent TYPE-C silica HPLC columns were introduced in March of 1992 and there have been many great applications for our silica hydride based approach to column technology. However, there has been no greater interest then the bio analytical application for metabolomics.
- Using the two columns in this kit, our unique Cogent Diamond Hydride™ column for all hydrodphilic and many hydrophobic metabolites and our Cogent Bidentate C18™ for remaining hydrophobic species, you can easily retain all metabolites using LCMS. Using both Aqueous Normal Phase (ANP) and Reverse Phase (RP) with these columns you can separate both positive and negative species and with the incredible precision that silica hydride columns provide, very accurate “metabolomes” can be produced as reported by Rhee et al.
- Precise enough for plotting accurate mass v. retention time, you can identify unknown metabolites or differences between healthy and diseases organisms or cells.
Each Kit Contains:
- 1 Cogent Diamond Hydride
- 2 Cogent Bidentate C18
- Available in most column sizes
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Total Method Development Kit
- The Cogent “Suite of Columns” is set of 5 columns of the same configuration consisting of one each of the Bidentate C18, UDC-Cholesterol, Bidentate C8, Diamond Hydride and Silica C. This kit provides an extremely wide spectrum of selectivies and possibilities. The completeness of the range is very convenient when results are elusive and a wider than normal range of possibilities is needed for results; quickly and immediately.
- Although each column has somewhat different properties than others in the suite and may overlap selectivity with some compounds on some of the columns in the suite there may be one column that retains longer, gives unique selectivity or produces the needed result when others in the suite do not. It is more than convenient to have these on hand when time is of the essence.
- Since each of the columns can be used in Reverse Phase, Normal Phase or Aqueous Normal Phase the range of possibilities is very wide. And all of the columns in the suite can be used at low pH or high pH (upper limit is pH 8 for all except the C18 and the C8 columns which can be used up to pH 10.2). All of the columns rapidly equilibrate between gradients or with initial use making them very quick into service.
Each Column that can do
Reverse Phase, Normal Phase, Aqueous Normal Phase
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Cogent Type B Silica Columns
Cogent EE, Cellulose Coated Chiral HPLC Columns
Features
- Increase Sample Load
- Improved Control Over Retention
- Rapid Equilibration - Less Memory
- Complimentary to other Chiral Columns
- Available in Lab Prep & Process Sizes as well
- The Cogent EE Chiral HPLC columns are a perfect complement to other cellulose carbamate coated columns for the separation of chiral compounds because of the unique character of these columns.
- Only with the Cogent EE Chiral columns can you predict retention based on the relationship between your target compounds solubility and the HPLC solvents resulting in an expanded dynamic range of resolution and solubility.
- Quickly change your mobile phase additives from acid to base and back to acid with little to no memory effect.
- Designed to work exceptionally well when used with mixed polar eluents in normal and polar organic phase but can also be used in reverse phase. This allows you to maximize eluent choice and enable multiple, high solubility options for chiral prep methods.
- Each column is supplied with quick start instructions including generic gradients for Normal Phase, Polar Organic Phase and Reverse Phase. Use the quick gradient screens to find an optimal isocratic method. Technical support is also always available.
- Caution: Do not use your current methodology with these columns are you may forfeit an optimal method possible only with Cogent EE Chiral HPLC columns.
Specification
- Macro porous spherical shaped, thermally treated type B silica; washed for low metals content, < 10ppm. Coated with proprietary cellulose carbamate technology.
- Better lot to lot consistency, unique selectivity; relationship produced between analytes and elution solvent. Gradient capable. Higher back pressure limit > 2,000 psi.
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Bonded Phase
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Nominal Size Distribution*
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pH
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Pore Diameter
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Particle Size
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Wet Bulk Density
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pH Range
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Max Temp
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Cogent EE™
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<1.6
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5 +0.5
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5nm
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5, 10 & 20µm
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0.45g/ml
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3.0–7.0
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30°C
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(* d50d10/d90)
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Cogent HPS reverse phase columns
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Bonded Phase
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Pore Size
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Surface Area
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Pore Volume
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Particle Size
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End-Capping
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Carbon Load
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pH Range
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Temp
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C18(ODS, MonoFunctional)
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120Ĺ
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300 m2/gm
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0.99mL/gms
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5.09µ
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Fully
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18.5%
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2.0 – 8.6
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60°C
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C8(Octyl, MonoFunctional)
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120Ĺ
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300 m2/gm
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0.99mL/gms
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5.09µ
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Fully
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11.5%
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2.0 – 8.6
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60°C
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Cyano(MonoFunctional)
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120Ĺ
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300 m2/gm
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0.99mL/gms
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5.09µ
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Fully
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7.5%
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2.0 – 8.6
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60°C
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Amino(TriFunctional)
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120Ĺ
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300 m2/gm
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0.99mL/gms
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5.09µ
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Not
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4.1%
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2.0 – 8.6
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60°C
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Phenyl
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120Ĺ
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300 m2/gm
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0.99mL/gms
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5.09µ
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Not
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12%
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2.0 – 8.6
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60°C
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Features
HPS specifications include:
- Monofunctional bonding
- Metals content that are so low, that they have virtually no impact on separation mechanisms as in older brands of silica based HPLC Columns.
- Particle size distribution that is so tight that no column offers greater efficiency
- Pore volume that is extremely consistent due the nature of our proprietary chemical process making resolution extremely reproducible.
- An incredibly uniform surface area and structure, always perfectly spherical!
- The Cyano phase columns are recommended for bases in reverse phase due to their highly retentive nature and tailing normally found in reverse phase chromatography.
- The Amino phase columns are excellent choices for non hexane normal phase chromatography and useful for highly acidic compounds by using Methanol or Acetonitrile. Also excellent choices for separation of sugars when using RI - Refractive Index Detectors.
- The Phenyl phase columns are recommended for separation of aromatic analytes such as brominated phenol
- Compares to Luna® Symmetry
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Cogent hQ
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Bonded Phase
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Pore size
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Surface Area
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Pore Volume
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Particle Size
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End Capping
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Carbon Load
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pH Range
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Max Temp
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Cogent hQ C18
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120 A
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300 m2/g
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0.99 ml/g
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5 µm
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not
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18 %
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2.0–6.0
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60°C
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Cogent hQ C8
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120 A
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300 m2/g
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0.99 ml/g
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5 um
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not
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11.5%
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2.0 - 60°C
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60°c
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Features
Excellent Chromatography Always Begins WithExcellent Silica Manufacturing
- The proprietary production of our high purity silica provides us with the control we need to ensure that each batch of silica is made to exacting standards. Our perfectly spherical particles with consistent pore volume and particle size distributions allows our chemists to consistently produce our “new-millennia grade” chromatographic columns and media.
hQ specifications include:
- Silica metal content that is so low, that there is virtually no impact on separation mechanisms as in older brands of silica based columns.
- Non end-capped “natural silanols” of the silica are part of the separation mechanism without producing peak tailing for many compounds.
- Particle size distribution that is so tight that no column maker offers greater efficiency.
- Pore volume that is extremely consistent due to the nature of our proprietary chemical process making resolution extremely reproducible.
- An incredibly uniform surface area and structure, always perfectly spherical!
HPLC Columns
- Cogent hQ HPLC Columns are designed for the discerning laboratory and are the result of years of product development and research, offering unique separation advantages due to non end-capping of our High Purity Silica. The natural, silanol activity acts as a valuable secondary separation mechanism producing a unique character.
- Due to the extremely low metals activity of our hQ product, these columns provide analyses with a more polar stationary phase than the average “Base Deactivated” column while still producing good peak symmetry for more polar compounds.
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Cogent C27 Longer Chain, Hydrophobic Columns
Structure
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Bonded Phase
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Pore size
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Surface Area
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Pore Volume
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Particle Size
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End Capping
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Carbon Load
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pH Range
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Max Temp
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Cogent UPHOLD C27
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120 A
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300 m3/g
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0.99 ml/
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5µm
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fully
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17%
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2.0–8.0
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60°C
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Features
- Consistant Retention in High Aqueous
- Versatile and Rugged
- Useful for Hydrophilic Compounds
- Cogent UPHOLD C27 HPLC Columns are designed to provide a unique separation mechanism for all compounds.They are particularly useful for retention of low molecular weight carboxylic acids and other highly polar compounds which require highly aqueous mobile phase.
Applications
Useful with Very High Water Content
- Most C18 columns suffer from declining retention over time when used with very high water content mobile phase (>95%). This loss of resolution is due to the “Fold-Back” phenomena which is the compression of the C18 chain caused by lack of wettability. UPHOLD C27 will give consistent results longer than C18 phases in these conditions as the structure of the stationary phase ligand minimizes folding.
Useful for a Wide Range of Compounds
- An increased retention may be found compared with other alkyl based columns and is very useful when increased retention of polar compounds is desired. For many other separations, UPHOLD C27 can easily be substituted for C18 columns with increased suitability for hydrophilic compound
- Consistant Retention in High Aqueous
- Versatile and Rugged
- Useful for Hydrophilic Compounds
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Cogent C30 Longer Chain, Hydrophobic Columns
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Bonded Phase
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Pore size
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Surface Area
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Pore Volume
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Particle Size
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End Capping
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Carbon Load
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pH Range
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Max Temp
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Cogent C30
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200 A
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3 or 5µm
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no
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18%
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2.0–8 .0
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40°C
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Features
- Cogent TYPE-B, HPLC materials are spherical in shape and produce higher column efficiencies because closely distributed silica particles can form more tightly packed beds thereby reducing the column void volume.
- C30 columns are useful for long-chain, mostly hydrophobic isomers. And are often a better alternative to normal phase columns for the same compounds since they do not suffer from the problems typical of normal phase HPLC. C30 columns have shown shape selectivity for these isomers.
- The Cogent C30 columns are available in a 200A pore size making them an excellent choice for large molecules such as carotenoids, peptides and some proteins.
- The particle sizes available for these columns is 5um or 3um and perform predictably with regard to column efficiency and back pressure.
- C30 columns are optimally used at ambient or lower temperatures to maintain selectivity.
Applications
A short list of some of the successful methods:
- Carotenoids
- Vitamins such as E and A
- Peptides
- Proteins
- Lipophilic compounds
- Foods and other Natural Products
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