Logochromatographyshopneu
Capcell from Shiseido

CAPCELL PAK® an innovative range of unique columns

Product range comprises

  • Capcell Pak AG and SG
  • Capcell Pak UG
  • Capcell Pak MG
  • Capcell Pak ACR
  • Capcell Pak AQ
  • Capcell Pak DD
  • Capcell Pak MF

 

CAPCELL PAK®

  • Polymer coated ‘capsule type’ silica
  • Extended pH range of 2 - 10
  • Durable and reproducible
  • Low column pressure

Capcell Pak columns are manufactured by Shiseido in Japan. The base silica gel is first homogeneously coated with a silicone polymer monolayer by vapour deposition. A range of phases is then produced by bonding to the silicone polymer coated surface. As a result, the appropriate functional groups are chemically bonded via a Si-C (Figure 1) rather than Si-O-Si linkage, providing enhanced pH stability and excellent reproducibility.

SHIS Fig 1
Fig 1

Products

Capcell Pak Phase

Particle Size (μm)

 Pore Size (┼)

Surface Area (m2/g)

Carbon Load (%)

C18 MG

3, 5

100

260

15

C18 ACR 

3, 5

 80

340

 18

C18 AQ

3, 5

120

300

11

C8 DD

5

 80

300

11

C18 UG  

3, 5

120

300

15

C8 UG

 5

120

300

10

C1 UG 

5

120

300

7

Phenyl UG

 5

120

 300

5

CN UG 

5

120

300

5

NH2 UG

5

 80

400

15

SCX UG  

5

80

400

9

C18 SG 

5

120

300

14

C8 SG  

5

120

300

10

C18 AG  

5

120

300

15

C8 AG

 5

120

300

10

 

 

Capcell Pak AG and SG Types

The Capcell Pak AG type was the first material produced using Shiseido's 'capsule type' bonding. Since then the product line has evolved in terms of quality of the base silica gel as well as the polymer coating technology. The SG type was developed utilising a higher purity silica base.Capcell Pak UG Series

The Capcell Pak UG type

 has similar retention characteristics to the SG series, but separates basic and polań compounds faster and with more symmetrical peaks than the SG type columns. A wide variety of chemistries is available

Capcell Pak MG Series

Capcell Pak C18 MG is recommended as the column of choice for developing new methods. It is suitable for separating both hydrophobic and polar compounds and is a robust and reproducible phase with a high alkaline and acid resistance. The 3 micron phase is ideal for high throughput analysis. Capcell Pak C18 MG S5 Mini is a short column for rapid, inert LC-MS applications. Capillary columns for peptide mapping and proteomics are now available. Figure 2 demonstrates the short LC-MS analysis times that can be obtained with this phase.

SHIS fig 2

Capcell Pak ACR Series

Capcell Pak C18 ACR has unprecedented acid resistance and enables the analysis of compounds requiring highly acidic mobile phases (as low as pH 1). It is ideal for LC-MS analysis under conditions of low organic solvent. Although the phase shows similar retention properties to those of UG, it also shows a high steric selectivity, analogous to polymeric phases.

Capcell Pak AQ Series

Capcell Pak C18 AQ has a high surface polarity, which results in strong retention and high performance separations of very polar compounds. The phase is stable in 100% water and shows high acid resistance. Figure 3 shows the separation of nucleic acid bases using 100% aqueous buffer.

SHIS Fig 3

 

Capcell Pak C8 DD

Capcell Pak DD (Double Durability) has unparalleled acid and alkali resistance. The high surface polarity and lower hydrophobicity compared to C18 columns, make this product ideal for fast analyses of mixtures with diverse hydrophobicities.

 

 

Capcell Pak MF

  • Direct injection of body fluids
  • High drug recovery
  • Very reproducible

Capcell Pak MF (mixed functional phase) allows direct injection of serum or other biological fluids. This is done by bonding hydrophilic polyoxyethylene groups and hydrophobic groups (phenyl, C8, C1, SCX) to the polymer coated silica. This allows proteins to be eluted in the void volume due to restricted access to the packing surface while retaining the
drug of interest (Figure 4).

SHIS Fig 4

 

© by LCC 1995 -2016 All rights reserved
 Chromatographyshop is a division of LCC Engineering & Trading GmbH, CH-4622 Egerkingen, Switzerland.
Collaboration partners in various countries
Our Company Profile and Summary Product List 2015
Unser Firmenprofil und Produktzusammenfassung 2015
      Our Terms and Conditions

Sample Preparation Tools

[Sample  Preparation]
[MatrixEx]
[QuEChERS]
[SPE]
[Syringe Filters]
[Inert RSA  Glass Vials]
[Flash Chromatography]

General Issues

[Chromatogr. Shop Home]
[Newsletter Archive]
[Our Services & Company]
[Terms & Conditions]
[Mobile Phone]

 Bonuses for you

[Special Deals for you]

Seminars & Workshops

[Knowledge Education]
[Seminars & Workshops]

Analytical Scale Chromatography

[Analytical Columns]
[ACE & ACE  Excel Columns]
[ACE Ultra Core]
[Microsolv Cogent Column]
[Capcell Shiseido]
[Daicel / Chiral Technologies]
[GL Science  Inertsil]
[Hichrom Columns]
[Imtakt Column]
[Kromasil Akzo Nobel]
[Mitsubishi MCI-Gel]
[Merck Lichro...]
[Macherey &  Nagel]
[Princeton  Chromatography]
[PolyLC  Columns]
[SieLC Primesep]
[Capcell Shiseido]
[Ultron by Shinwa]
[Welch  Materials]
[Zirchrom]
[Agilent Zorbax]

Knowledge Tools for Chromatographers

[Knowledge Education]
[Definition of  Terms Used]
[Separation Mechanism]
[Solvent  Selection]
[Buffers for  LC.]
[Column &  Material Care]
[Prep & Process Columns]
[Analytical  Columns]
[HPLC- to-UHPLC]

Resin based Systems (Analytical to Process scale)

[Bioseparation Biopurification]

Special Tools to improve data quality, reproducibility and productivity

[Inert RSA  Glass Vials]

Method & Material  Choices  1

[Knowledge Education]
[Sample  Preparation]
[Reverse_Phase_LC]
[Adsorption-LC RPC & SPE]
[AQ Columns]
[Phenyl Bond Columns]
[FPF_Columns]
[HILIC & Aq. Norm. Phase]
[Electrostat. Repul. HILIC]
[Normal Phase Chromatography]

Process Scale   Chromatography Equipment

[Process  Chromatogr.]
[Process Chromat.Systems]
[Flash &  MPLC Pumps]
[HPLC  Pumps]
[Special  Pumps]
[Fraction Collectors]
[Stainless Steel Columns]
[Glass  Columns]
[Glass  Cartridges]
[Plastic Cartridges]
[Manual  Valves]
[Motor  Valves]
[Efficiency  Tools]

Method & Material Choices 

[Knowledge Education]
[Multi  Mode LC]
[Chiral  Chromatography]
[Ion Exchange  & Ion LC]
[Hydrophobic  Interaction LC.]
[Affinity  LC]
[Size Exclusion Chromatogr.]
[SuperCritical Fluid LC]
[Proteomics]
[Metabolomics]
[Process  Scale LC]
[Kamasutra Inspired Tech]