Gift  for Enduser
A limited number of Leathermans will be offered for any order over CHF. 3000.-. from end-user. This offer is valid until 30^th April 2012.

Geschenk an Chromatographen

Wir offerieren eine beschrankte Anzahl von Leadermans für Bestellung von > CHF 3000.- bis zum 30. April  2012

Most popular and reliable HPLC columns in the pharmaceutical industries!

Who is  ACE

ACE columns are  manufactured  by Advanced Chromatography Technologies (ACT) and launched into the market by 2000. ACE is considered by many chromatography experts as one of the very best columns available. ACE Chromatography materials consists of a range of ultra pure silica in a wide range of bonding chemistries and particles. ACE is based on a  new generation ultra pure base deactivated silica.

To place an order or enquiry please click here or on button “Contact”

ACE Columns are for normal pressure HPLC and ACE Excel Columns for uHPLC and SFC operations.

The ACE product range comprises of:

• ACE Reverese Phase Columns (C18, C8, C4
• ACE Polar Bonded Columns (CN
• ACE Silica -Normal Phase Columns (SIL)
• ACE Phenyl Bonded Phase Columns (Phenyl)
• ACE Highly Aequeous Reverse Phase Columns ((AQ , C18-AR)
• ACE Fluorinated Columns (C18-PFP)
• ACE High Load Column(C18-HL)
• ACE Wide Pore (Biochromatography ) Column
• ACE Column Kits
• ACE LC-MS and Rapid Analysis Columns
• ACE Capillary and Nano-Column
• ACE Prep Column

ACE ExCEL Product Range for uHPLC and SFC chromatography

Ultra robust columns - Unique phases - High efficiency 2 um particle. Designed to take full advantage of low dispersion, ultra-high pressure UPLC® and UHPLC instruments, the introduction of ACE® ExcelTM UHPLC columns gives chromatographers morechoices to achieve better results. Go for it!

• ACE Excel Column ( C18, C18-AR, C18-PFP, C8, C4, CN, Phenyl, AQ, SIL, C18 HL)

General Product Benefits

• Excellent performance : up to 200,000 plates/metre
• Very popular column in the market
• Column lengths : 1cm - 25cm
• Column diameters: 1.0mm - 30.0mm
• Particle sizes: 3µm, 5µm, 10 µm and 15µm
• Pore sizes: 100Å and 300Å
• Range of bonded phases : C18, C8, C4, CN, Phenyl, AQ, C18-HL, C18-AR, C18-PFP
• Fully validated Columns
• Excellent for LC/MS and rapid analysis applications
• The ultra inert characteristics of all ACE phases make them the ideal choice for separating polar basic compounds.
• ACE columns consistently produce outstanding peak shape and column efficiency when separating troublesome basic compounds.
• ACE High Purity Base Deactivated Silica guarantee reproducibility, excellent column lifetime and excellent performance with acidic, basic and neutral molecules by the most stringent of validation protocols.
• Independent comparison tests show ACE HPLC columns give outstanding performance. I

ACE Catalogues :

ACE_Catalogue_2009_10

ACE_Catalogue_2011_12

ACE_batch_validation_report

ACE_test_chromatogram

For chromatograms please select frm the following list :ACE_Application_Index

ACE 100 A  Material

• ACE bonded phases are available in 3, 5, 10 and 15µm particle sizes, so regardless of the application, the method can be scaled up or scaled down and the same selectivity be assured. For fast, high resolution preparative chromatography, a range of pre-packed ACE preparative and combinatorial chemistry columns are available.
• Method development is recommended to start with C18 or C8, knowing that if more retention and hence more resolution is needed, the next choice is C18-HL (Hi-Load). Starting with C8 offers the benefit of shorter analysis times and/or lower organic solvent use. The elution order for most compounds will be the same on the aliphatic (C18, C8, C4) phases. If a different elution order is required for compound verification or to resolve matrix components, changing to a phenyl or CN phase is simpler than trying to change selectivity by eluent changes. In many cases, the ACE CN and ACE Phenyl phases will offer a significant difference in selectivity from the aliphatic phases.
• Consider also ACE LC-MS and rapid analysis columns .
• Scale up or Scale down

Reverse Phase Columns

ACE C18 Columns

Description:

• Optimised for maximum efficiency,
• superior peak shape and resolution. Utilises the same ultra high purity silica as all ACE phases.

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C18 Octadecyl Yes 3,5,10 100 300 15.5

Application:

• Recommended starting point for method development.
• Also suited to high aqueous conditions and for rapid analysis applications.
• A C18 phase for most HPLC applications.
• Available in a range of particle sizes,
• From LC-MS and microbore applications through to preparative scale separations

Description

• Increased bonding density compared to ACE C18. 
• Similarly optimised for maximum efficiency, superior peak shape and resolution.

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C8 Octyl Yes 3,5,10 100 300 9

Application:

Recommended starting point for method development. Also suited to high aqueous conditions and for rapid analysis applications.

Description

• Combines lower hydrophobicity with excellent chromatographic performance. Improved hydrolytic stability compared to conventional C4 phases.

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C4 Butyl Yes 3,5,10 100 300 5.5

Application:

• Use for rapid analysis optimisation, when less retention than C8 or C18 is required.
• Also suitable for analysis of small proteins.

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ACE CN Columns

Description:

• Suitable for use in both normal- and reversed phase modes.
• Greatly improved performance, stability and reproducibility compared to conventional CN phases

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE CN Cyano Yes 3,5,10 100 300 5.5

Applcations

• Use to increase retention of polar compounds.
• Ideal for gradients and rapid screening applications due to fast equilibration capacities

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Description

• Non-bonded silica for use in normal-phase mode

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE SIL   Yes 3,5,10 100 400

Application

• Useful for separation of isomeric compounds.Specialty Products

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Phenyl bonded Phases

ACE Ph Columns

Description

• Hydrophobicity between C4 and C8 phases, with increased polar selectivity.
• Improved performance, stability and reproducibility compared to conventional phenyl phases.

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE Ph Phenyl Yes 3,5,10 100 300 9.5

Application

• Use for rapid analysis optimisation, when less retention than C8 or C18 is required.
• Also suitable for analysis of small proteins.

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ACE AQ  Columns

Description

• A C18 bonded phase with integral polar functionality
•  Retains polar compounds in 100% aqueous eluent
• Unique C18 bonded phase with integral polar functionality
• Resistant to retention loss in 100% aqueous eluent
• Rapid gradient re-equilibration (0-100%)
• Excellent peak shape with acidic, basic and neutral molecules
• Ideal for LC-MS applications
• Unmatched reproducibility

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE AQ Proprietary Yes 3,5,10 100 300 14

Application:

• Recommended for applications where 100% aqueous eluents are required
• ACE AQ is an ultra-inert phase designed for the reversed-phase separation of very polar compounds with up to 100% aqueous eluent. ACE AQ is ideal for use with fast gradients due to its rapid re-equilibration proper Maximum Reproducibility in High Aqueous Eluents ACE AQ is particularly recommended for applications requiring high aqueous content eluents. Improved resistance to phase collapse is seen compared to standard C18 phases.
• When separating very polar, water-soluble compounds, highly aqueous (>95%) eluents are often required to achieve sufficient retention. However, operating a conventional C18 column under such conditions can lead to poor chromatographic reproducibility. Over time peaks will elute with shorter and shorter retention times and resolution between peaks will deteriorate.
• ACE AQ columns introduce an integral polar functionality, which prevents this retention loss when using highly aqueous mobile phases.rties and resistance to retention loss.
• Increased Resolution with 3µm Columns
• With today's increased pace of drug discovery, fast and efficient methods are required. Short, narrow-bore columns are replacing the conventional 250 x 4.6mm versions. ACE HPLC columns are available in both 3 and 5µm particle sizes. Although 5µm particles are sufficient for most applications, greater efficiency can be obtained by using smaller particles. This increased efficiency enables the use of shorter (<150mm) column lengths, resulting in decreased analysis times. Due to the excellent flow characteristics of ACE silica, the high back pressures often encountered with other columns are eliminated.

Discription

• ACE C18-AR: a C18 bonded phase with unique selectivity
• Alternate selectivity to “standard” C18 columns – additionally recommended for compounds with aromatic functionality
• Ultra inert, ultra high purity silica, for excellent peak shape and reproducibility
• Compatible with highly aqueous mobile phases to enable the retention and separation of polar compounds
• Exceptional bonded phase stability for elevated temperature applications
• Availability in high throughput column dimension

Phase Functional Group Encapped Particle Size Pore Size Surface Area Carbon Load Maximum pH Range ACE C18-AR Proprietary octadecyl with hydrophobic & aromatic "mixed-mode" interaction yes 3,5,10 100 300 15.5 1.5-10.0

Application

• Improved chromatographic resolution

ACE- C18-AR Brochuree

:ACE-AR_Brochure-WEB

ACE_C18AR_Steroids

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ACE C18-PFP

Description

• ACE C18-PFP: a C18 bonded phase with extra resolving poweh
• Combines C18 & PFP mechanisms of separation to separate mixtures not possible with either phase alone
• Ultra inert, ultra high purity silica, for excellent peak shape and reproducibility
• Exceptional bonded phase stability for elevated temperature applications
• Ultra low bleed phase ensures UV and LC/MS compatibility
• Availability in high throughput column dimensions

Phase Functional Group Encapped Particle Size Pore Size Surface Area Carbon Load Maximum pH Range ACE C18-PFP Proprietary octadecyl with embedded PFP functionality yes 3,5,10 100 300 14.3 1.5-10.0

Application

• Useful for separation of isomeric compounds

AC C18 PFP Brochure:

 ACE_C18PFP_brochure

ACE_C18-PFP-Guaranteed_Reproducibility

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ACE High Load Phases / Columns

ACE C18- HL (High-Load)

Description

• Higher carbon load than ACE C18 leading to increased retention times and higher loadability.
• High surface area, high carbon load phase
• 3, 5, 10 and 15µm particle sizes
• Increased loading and retention
• Exceptional chemical stability 
• Excellent peak shape with acidic, basic and applications neutral molecules

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C18 - HL Octadecyl Yes 3,5,10,15 100   20

Application

• Availability of particle sizes up to 15µm ensures easy scale-up for preparative and process scale applications.
• Optimised for preparative and process scale
• The increased retention characteristics of ACE C18-HL make  it an ideal selection for LC-MS applications. Retention can be  maintained whilst reducing the aqueous content of the eluent  thus increasing sensitivity, .
• For preparative applications, the higher surface area leads to increased loading capacity. Available in 3, 5, 10 and 15µm particle sizes and a wide range of column dimensions. ACE C18-HL columns show reproducible scale up from LC-MS to preparative dimensions

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ACE 300A  Columns for Biotechnology  Application

Benefits:

1. 300 A ultra high purity silica
2. Up to 50 KDa MW large molecules
3. Ultimate peptide and protein application column
4. C18, C8, C4, CN and Phenyl chemistries
5. 3.5. and 10 um particle sizes
6. Unmatched reproducibility
7. Exceptional chemical stability

Brochure: ACE_300A_For_RP_HPLC_of_Biomolecules

ACE C18 - 300

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C18 - 300 Octadecyl Yes 3,5,10 300 100 9

Application: Peptides

ACE C 8 - 300

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C8 - 300 Octyl Yes 3,5,10 300 100 5

Application : Peptides and Proteins

ACE C4 - 300

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE C4- 300 Butyl Yes 3,5,10 300 300 2.6

Applications: Peptides and Proteins

ACE CN - 300

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE CN - 300 Cyano Yes 3,5,10 300 300 2.6

Applcations

ACE Ph - 300

Phase Functional Group Endcapped Particle Size um Pore size  A Surface  Area sqm/g Carbon Load % ACE Ph - 300 Phenyl Yes 3,5,10 300 100 5.3

Application

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Complete Method development Kit 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 3 um Complete Method development Kit (C18, C8, C4, CN, Ph phases) ACE-MDK3-0546 ACE-MDK3-1546   5 um Complete Method development Kit (C18, C8, C4, CN, Ph phases) ACE-MDK5-0546 ACE-MDK5-1546 ACE-MDK5-2546 Additional column dimmension are available       Hydrophobic Selectivity Kits 50 x 4.6  mm 150 x 4.6 mm 250 x 4.6 mm 3 um Hdrophobic Selectivity Kit (C18, C8, C4 phases) ACE-HSk3-0546 ACE-HSK3-1546 ACE-HSK3-2546 5 um Hydrophobic Selectivity  Kit (C18, C8, C4 phases) ACE-HSK5-0546 ACE-HSK5-1546 ACE-HSK5-2546 Polar Selectivity Kits 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 3 um Polar Selectivity Kit (C18, CN, Ph phases) ACE-PSK3-0546 ACE-PSK3-1546 ACE-PSK3-2546 5 um Polar Selectivity Kit (C18, CN, Ph phases) ACE-PSK5-0546 ACE-PSK5-1546 ACE-PSK5-2546 C18 Selectivity Kits 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 3 um C18 Selectivity Kit (C18, C18-HL, C18-300 phases) ACE-CSK3-0546 ACE-CSK3-1546 ACE-CSK3-2546 5 um C18 Selectivity Kit (C18, C18-HL, C18-300 phases) ACE-CSK5-0546 ACE-CSK5-1546 ACE-CSK5-2546 LC-MS Method development Kit 20 x 2.1 mm 30 x 2.1 mm 50 x 2.1 mm 3 um LC-MS Method Development  Kit (C18, C8,  C4, CN, Ph  phases) ACE-MDK3-0546 ACE-MDK3-1546 ACE-MDK3-2546 5 um LC-MS Method Development  Kit (C18, C8,  C4, CN, Ph  phases) ACE-MDK5-0546 ACE-MDK5-1546 ACE-MDK5-2546 Method Validation Kit 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 3 um Method Validation Kit (three different batches of the same phase) enquire     5 um Method Validation Kit (three different batches of the same phase)       Conmbinatorial Column Chemistry Kit 50 x   7.75mm and 50 x 4.6 mm columns 50 x 10 mm and 50 x 4.6 mm columns 50 x 21.2  and 50 x 4.6 mm columns Conmbinatorial Column Chemistry Kit (Contains matched analytical and preparative /semi prep columns)

Feature

• High performance - excellent peak shape for higher sensitivity
• Choice of 13 low bleed phases for complete optimization
•  Ultra-inert silica enables MS compatible buffers to be used
•  20, 30, 35 and 50 mm column lengths
• 1.0, 2.1, 3.0, 4.0 and 4.6 mm i.d.s
• Nowhere is the need for a truly ultra-inert base deactivated HPLC column more important than in high-throughput analysis or LC-MS. Even subtle differences in silanol activity between columns can markedly affect the chromatography in the very short, fast columns typically used. In addition, any peak tailing due to silanol activity can have a profound effect on detection limits in high sensitivity assays.
• ACE ultra-inert base deactivated HPLC columns virtually eliminate the negative effects of silanols in HPLC separations. This unequalled performance is now available in 20, 30, 35 and 50mm length columns, with diameters from 1.0 to 4.6mm. The ACE columns are suitable for high throughput and LC-MS applications, and are the ideal choice for high volume screening assays used for drug analysis and combinatorial libraries where robust, reproducible columns are essential.
• Increased Column Robustness
• ACE LC-MS and rapid analysis columns are manufactured by a unique process which results in increased column bed stability, thus increasing column lifetime.  
• LC-MS Buffer Compatibility
• ACE LC-MS columns have an extremely low requirement for buffer salts or modifiers in order to achieve good peak shape. ACE columns have been demonstrated to be extremely robust at high and low pH. This broad pH operating range enables thorough optimization of pH and simplifies method development to a single column rather than selecting 3 different columns with low, intermediate and high pH stability.
• For maximum LC-MS compatibility under acidic conditions organic acids such as formic, acetic and TFA are recommended. Under basic conditions ammonium bicarbonate, ammonium acetate and ammonium hydroxide buffers are recommended.

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 ACE Capillary and Nano Columns

• High sensitivity
• Low sample mass and volume application
• Compatible with LC-MS and LC-MS/MS
• Very low solvent consumption
• Suitable for proteomics
• Compatible with LC-MS and LC-MS/MS

Brochure: ACE_Nano and LCMS Columns

ACE Capillary Column

Phase Column Diameter 50mm 100mm 150mm 250mm* Ace 3µm - 100Å           Ace 3 C18 0.3mm ACE-111-05003 ACE-111-10003 ACE-111-15003 -   0.5mm ACE-111-05005 ACE-111-10005 ACE-111-15005 - Ace 3 C8 0.3mm ACE-112-05003 ACE-112-10003 ACE-112-10003 -   0.5mm ACE-112-05005 ACE-112-10005 ACE-112-15005 - Ace 5µm - 100Å           Ace 5 C18 0.3mm ACE-121-05003 ACE-121-10003 ACE-121-15003 ACE-121-25003   0.5mm ACE-121-05005 ACE-121-10005 ACE-121-15005 ACE-121-25005 Ace 5 C8 0.3mm ACE-122-05003 ACE-122-10003 ACE-122-15003 ACE-122-25003   0.5mm ACE-122-05005 ACE-122-10005 ACE-122-15005 ACE-122-25005 Ace 3µm - 300Å           Ace 3 C18-300 0.3mm ACE-211-05003 ACE-211-10003- ACE-211-15003 -   0.5mm ACE-211-05005 ACE-211-10005 ACE-211-15005 - Ace 3 C8-300 0.3mm ACE-212-05003 ACE-212-10003 ACE-212-15003 -   0.5mm ACE-212-05005 ACE-212-10005 ACE-212-15005 - Ace 5µm - 300Å           Ace 5 C18-300 0.3mm ACE-221-05003 ACE-221-10003 ACE-221-15003 ACE-221-25003   0.5mm ACE-221-05005 ACE-221-10005 ACE-221-15005 ACE-221-25005 Ace 5 C8-300 0.3mm ACE-222-05003 ACE-222-10003 ACE-222-15003 ACE-222-25003   0.5mm ACE-222-05005 ACE-222-10005 ACE-222-15005 ACE-222-25005

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Benefits:

• Ultra high purity base deactivated silica
• 5, 10 and 15 um particles
• Fully validated column
• Exceptional reproducibility
• Excellent efficiencies
• High sample recovery
• Excellent column lifetime
• 100 A and 300 A poresizes

Please send enquiy.

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ACE EXCEL Columns

ACE_EXCEL_Product_Brochure

ACE Excel is a ultra high pressure column  system available in a three different particle diameter, all the well proven ACE surface chemistry packed in a wide range of cartridges.

Feature Explanation NEW high efficiency 2µm ACE particles ACE Excel UHPLC columns are available in a new high efficiency 2µm particle size. Selectivity is unchanged from existing 3µm, 5µm and 10µm ACE HPLC phases. Excellent peak shape, great column-to-column reproducibility and exceptional column ruggedness is now available to UPLC and UHPLC users with ACE Excel UHPLC columns HSCTM High Stability Columns All ACE Excel UHPLC columns are manufactured using a proprietary HSCTM (High Stability Column) manufacturing process that results in ultra robust UHPLC columns Optimised UHPLC hardware ACE Excel columns utilise specially developed low dispersion hardware which enables high efficiency UHPLC separations up to a maximum pressure limit of 1,000 bar (15,000psi). Unique stationary phases ACE Excel 2µm columns are available with C18-PFP and C18-AR "Extra Resolving Power" phases. These two unique phases offer multiple mechanisms of separation and have been specifically developed to provide alternative selectivity to C18 phases. ACE Excel 2µm columns are also available with C18, C8, C4, CN, AQ, Phenyl and Silica phases. Better UHPLC results The introduction of ACE Excel UHPLC columns means chromatographers now have more choices within their reach to achieve better results with their UPLC and UHPLC instruments Performance boost for HPLC instrumentation ACE Excel 2µm UHPLC columns provide significantly lower back pressure than other UPLC and UHPLC columns packed with <2µm particles. Therefore ACE Excel 2µm columns may also be used on HPLC systems to provide a performance boost compared to standard HPLC columns (subject to HPLC system pressure limitations). Maximise column lifetime/reduce analysis costs High performance, coupled with the lower back pressures of ACE Excel UHPLC columns, allow you to maximise UHPLC column lifetime, making ACE Excel UHPLC columns an excellent first choice when you need a cost effective, robust, high performance UHPLC solution.

Flyer: ACE_Excel_flyer

Custom design your own ACE Excel UHPLC colum as follows:

1. decide which surface chemistry and particle size you require.

2. Make a note of the resultant EXL number: e.g EXL-101 for 2 um C18

Surface chemistry C18 C18-AR C18-PFP C8 C4 CN Phenyl AQ SIL C18-HL ACE Excel 2um phase EXL-101 EXL-109 EXL-1010 EXL-102 EXL-103 EXL-104 EXL-105 EXL-106 EXL-107   ACE Excel 3um phase EXL-111 EXL-119 EXL-1110 EXL-112 EXL-113 EXL-114 EXL-115 EXL-116 EXL-117 EXL-311 ACE Excel 5um phase EXL-121 EXL-129 EXL-1210 EXL-122 EXL-123 EXL-124  EXL-125  EXL-126 EXL-127  EXL-321

3. Add beside the EXL number the Cartridge dimension number and click here to send us your request

Column length       Column diameter 20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mm 250mm 300mm UHPLC reuseable column connector UHPLC precolumn filter 2.1mm -0202U -0302U -3502U -0502U -7502U -1002U -1202U -1502U -2502U enquire EXL-CC10 EXL-PCF(10pk) 3.2mm -0203U -0303U -3503U -0503U -7503U -1003U -1203U -1503U -2503U enquire 4.6mm -0246U -0346U -3546U -0546U -7546U -1046U -1246U -1546U -2546U enquire

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