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Hydrophilic  Interaction Chromatography (HILIC) is a variant of normal phase chromatography  which is performed using polar stationary phases whith partially aequeous eluents. The technique combines the characteristics of 3 major liquid chromatography techniques – reverse phase- normal phase and ion chromatography. HILIC is an alternative approach to reverse phase for the effective separation of polar compounds. Solutes elute in order of hydrophilicity (polarity) the opposite of reverse phase thus providing an orthogonal selectivity.

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Description of HILIC Processes

For Start-up Methods please vist our Method archives below

Application field 1: High selectivity analytical HPLC methods for clinical, forensics and bioanalytics. Used in  biochemistry, metabolism, serum injections and all type of saccharides. Click link below

Clinical – Forensic – Bioanalysis Methods

Application field 2: Analytical methods for Environmental and agricultural compounds .  This field is local and not globally oriented. However these are excellent start up samples. Click link below

Methods for environmental and agricompound analysis

Application field 3: Methods for food purity and efficacy in nutrition are of global concern. Governments try to regulate food & nutrition through wide range of methods. Click link below

Methods for food-beverage-nutrition analysis

Application field 4: Methods for active ingredients drug compounds and admixtures in pharmaceutical Research & Development and Production. Click link below

Methods for active ingredients and pharmaceutical compounds

Application field 5:  Methods for mass spectrometry and other instrumental detectors provide additional separation and high resolution quantification.Click link below

Methods for Mass Spec and other high resolution detectors

Application field 6: Analytical methods for amino acids, peptides and proteins are important for separation, purification and quantification of complex bio related compounds. Click link below

Methods for Amino Acides-Peptides-Proteins

Application field 7: Wide range of botanical compounds in a wide range of matrixes . Sample Preparation and Analytical Methods for botanical compounds. Click link below


Application field 8: Innovation creates and requires novel synthetic polymers industial chemicals and miscellaneous compounds. Here you discover many unique methods. Click link below

Methods for synthetic Polymers and novel industrial compounds

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